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AAT Bioquest

What are the pros of ultrafiltration exosome isolation techniques?

Posted July 21, 2023


Answer

Ultrafiltration relies isolates exosomes based on their size. 

Pros of ultrafiltration exosome isolation technique:

  • These techniques are relatively simple, straightforward, and quick. 
  • They offer researchers a fair amount of flexibility in terms of pore size selection. Researchers can easily isolate exosomes within specific size ranges or exclude specific-sized particles by using membranes of different pore sizes. 
  • Minimal sample handling in these techniques which reduces the chances of sample loss or contamination. 
  • Ultrafiltration methods can be easily scaled up to process larger volumes of samples, making the preferred option when isolating exosomes for applications requiring larger quantities of exosomes.
Additional resources

Low pH increases the yield of exosome isolation

DiOC16(3) perchlorate [3,3-Dihexadecyloxacarbocyanine perchlorate]

DiR iodide [1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide]