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What are the steps involved in probe-based qPCR?
Posted May 12, 2023
DNA and RNA QuantitationDNA and RNANucleic AcidsNucleusPCRPCR Detection of Viral DNA/RNAPolymerase Chain Reaction (PCR)Real-Time PCR (qPCR)Reverse Transcription PCR (RT-PCR)Types of PCR
Answer
Probe-based qPCR uses fluorescent-labeled target-specific probes to measure DNA amplification at each cycle of a PCR. Probe-based dectection involves 4 main steps:
  1. Isolation of DNA 
  2. Denaturation
  3. Primer annealing and extension
  4. DNA synthesis and fluoresence detection
Additional resources
Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs Real-Time PCR (qPCR) ROX Reference Dye *50X fluorescence reference solution for PCR reactions*

How does Real-Time PCR work?What is the significance of the cycle threshold?What is the workflow of real-time PCR?What are the advantages of Real-time PCR over PCR?What are the similarities between primers and promotors?