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What are the steps involved in probe-based qPCR?
Posted May 12, 2023
Answer
Probe-based qPCR uses fluorescent-labeled target-specific probes to measure DNA amplification at each cycle of a PCR. Probe-based dectection involves 4 main steps:
- Isolation of DNA
- Denaturation
- Primer annealing and extension
- DNA synthesis and fluoresence detection
Additional resources
Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
ROX Reference Dye *50X fluorescence reference solution for PCR reactions*