Staining helps to increase the contrast between microorganisms and the background, enabling researchers to study the structural details of the microbe at higher magnification.
Based on the types and number of dyes used, the top staining can be categorized into the following types:
Gram staining– Gram staining uses two stains to differentiate bacteria into two categories - Gram positive and Gram negative. It serves as a vital tool in the initial characterization and classification of bacteria, aiding in the identification of bacterial pathogens through their Gram reaction (positive or negative) and morphological traits (cocci/rod).
Acid-fast staining- Acid-fast staining using the Ziehl-Neelsen technique is used to stain Mycobacterium species. It helps to differentiate between acid-fast bacteria and non-acid fast bacteria, which do not stain well by the Gram staining.
Endospore staining – This differential staining technique helps to distinguish the relatively few species of endospore-producing bacteria from those that don’t produce endospores. Endospore staining highlights the spore structure as well as free spores.
Acridine orange staining– Acridine orange binds to nucleic acid and stains them, enabling the detection of mycoplasmas or bacteria that do not have a cell wall. This staining technique is useful for detecting the presence of bacteria in blood cultures when Gram stain results are difficult to interpret or when light microscopy results are indistinguishable.
Capsule staining– Capsule staining confirms the presence of capsules in certain species of bacteria and yeasts.
Cytoplasmic inclusion staining– This staining technique is used to detect the presence of intracellular deposits of substances such as glycogen, starch, hydroxybutyrate, and polyphosphates among others.