AAT Bioquest

What does FITC bind to?

Posted November 10, 2022


FITC (Fluorescein isothiocyanate) labels proteins. 

FITC is a widely used fluorescence labeling reagent. It forms a covalent amide bond on reacting with a free primary amine group on the protein. The primary amine interacts with the electrophilic carbon of the isothiocyanate group, resulting in the formation of a thiourea bond, which binds the FITC to the protein. 

Additional resources

FITC-Labeled Alendronate as an In Vivo Bone pH Sensor

Fluorescein isothiocyanate (FITC)

5-FITC [Fluorescein-5-isothiocyanate] *CAS 3326-32-7*