Products
Technologies
Applications
Services
Resources
Selection Guides
About
What does FITC bind to?
Posted November 10, 2022
Antibodies and Proteomics Chemical ReagentsAntibody and Protein LabelingClassic DyesFlow Cytometry ReagentsFluoresceinsFluorescenceImmunohistochemistry (IHC)immunocytochemistryin situ hybridization
Answer
FITC (Fluorescein isothiocyanate) labels proteins.
FITC is a widely used fluorescence labeling reagent. It forms a covalent amide bond on reacting with a free primary amine group on the protein. The primary amine interacts with the electrophilic carbon of the isothiocyanate group, resulting in the formation of a thiourea bond, which binds the FITC to the protein.