AAT Bioquest

What does Taq buffer do in PCR?

Posted August 14, 2023


Taq buffer with MgCl2 provides an optimal and stable chemical environment that allows the DNA polymerase enzyme to work efficiently in the PCR process. 

Taq buffer is a crucial component in PCR, a technique that’s used to produce multiple copies of a specific DNA sequence. The buffer contains various chemicals that help regulate the pH level and also provide essential elements for the enzyme to work correctly so that the DNA can be copied and amplified correctly. 

Additional resources

Impact of DNA Polymerases and Their Buffer Systems on Quantitative Real-Time PCR

Polymerase Chain Reaction (PCR)

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