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AAT Bioquest

What factors should I consider when choosing a protein assay?

Posted October 6, 2022


Answer

When choosing a protein assay, there are many different factors to consider. They are listed in the bulleted points below.

  • Interfering agents (e.g., acetone, methanol, and ethanol)
  • Assay sensitivity
  • Time considerations
  • Sample preparation, size, and dilution
  • Protein standards and protein-to-protein variations
  • Instrumentation requirements 
Additional resources

Protein Determination—Method Matters

Amplite® Colorimetric Bradford Protein Quantitation Assay Kit

Protein to Protein Conjugation