What is prime editing? | AAT Bioquest

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What is prime editing?

Posted March 21, 2024

Cellular Structures and Organelles DNA and RNA Quantitation Gene Expression Analysis & Genotyping Genome Editing

Answer

Prime editing is a gene-editing technique that combines the precision of the CRISPR-Cas9 system with the capability of a reverse transcriptase enzyme. This enzyme utilizes an RNA template to synthesize new DNA sequences and insert them into the genome.

Additional resources

Prime editing: advances and therapeutic applications Genome Editing & CRISPR Transfectamine™ 5000 Transfection Reagent

What is base editing?What are the differences between base editing and prime editing?What is multiplex genome editing (MGE)?What are the components of a typical prime editor?How do I analyze CRISPR editing results?