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What is the difference between Firefly and Renilla Luciferase?
Posted July 14, 2022
EnzymesLuciferasesCell MetabolismCellular Processes
Answer

Firefly luciferase assay uses luciferin in the presence of oxygen, ATP and magnesium to produce Green/Yellow light of 550-70 nm. Renilla luciferase assay requires only coelenterazine and oxygen to produce Blue light of 480 nm. 

Additional resources
Optical imaging of Renilla luciferase, synthetic Renilla luciferase, and firefly luciferase reporter gene expression in living miceLuciferasesAmplite® Gaussia Luciferase Reporter Gene Assay Kit *Maximized Luminescence*

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