Luciferase assays have a wide range of applications including but not limited to:
Study of cell signaling pathways
Study of RNAi and non-coding RNAs
Analysis of gene expression
Analysis of promotor structure
Analysis of functional SNPs for the promoter
Detection of the level of cellular ATP
Non-invasive gene labeling in live animals
There are several different luciferase assays to choose from including flash assays, glowing assays, single assays and dual luciferase assays. The right one to use will depend on what is needed for the experiment.