AAT Bioquest

Does hydrolysis release energy?

Posted May 9, 2023


Yes, ATP hydrolysis releases energy. 

ATP (adenosine triphosphate) has three phosphate groups that are linked to each other by high-energy phosphoanhydride bonds. During hydrolysis, one phosphoanhydride bond is broken, releasing one phosphate group along with an appreciable amount of energy that was stored in the bond.

Additional resources

ATP hydrolysis assists phosphate release and promotes reaction ordering in F1-ATPase

PhosphoWorks™ Luminometric ATP Assay Kit *DTT-Free*

PhosphoWorks™ Fluorimetric ADP Assay Kit *Red Fluorescence*