AAT Bioquest

What is the limitation of the bacteria protein expression system?

Posted August 5, 2023


One limitation is that there are difficulties in expressing certain mammalian proteins as they possess complex structures. Another disadvantage is the accumulation of proteins as inclusion bodies that arise from the misfolding of proteins. Endotoxin accumulation during the experiment may cause significant biological effects on the experiment. Potential protease contamination from host proteins can lead to degradation of expressed protein.

Additional resources

Impact of the Expression System on Recombinant Protein Production in Escherichia coli BL21

Antibodies and Proteomics