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AAT Bioquest

What is the optimal pH for using proteinase K?

Posted September 7, 2023


Answer

The optimal pH for using proteinase K is 8.0. In solution, proteinase K can be used in a wide range of temperatures and buffers between 25 and 65 degrees celsius and a pH of 7.5-12.0.

Additional resources

Proteinase K

Enzymes

Amplite® Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*