logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
Does EDTA inactivate proteinase K?
Posted September 7, 2023

Answer

No, EDTA does not inactivate proteinase K. EDTA is used along with proteinase K in order to inhibit metal-ion dependent enzymes such as nucleases. It does not directly affect enzyme activity. Proteinase K is a highly stable protease, and is inactivated through heat at a temperature of 95 degrees celsius for 10 minutes. It is important to note that the addition of EDTA may impact calcium and thus lessen proteinase K activity to a certain degree. This is due to the relationship of calcium and the stability of proteinase K.