Calculator
What is the optimal temperature for activating proteinase K?
Posted September 7, 2023
Answer
The optimal temperature for activating proteinase K is 50-65 degrees celsius. Higher temperatures facilitate the unfolding of contaminant proteins and nucleases, and increase the ability of proteinase K to neutralize unwanted proteins.
Additional resources
Characterization of thermal denaturation process of proteinase K by spectrometry
Amplite® Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*