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What is the optimal temperature for activating proteinase K?
Posted September 7, 2023
Antibodies and ProteomicsEnzymesProtein
Answer

The optimal temperature for activating proteinase K is 50-65 degrees celsius. Higher temperatures facilitate the unfolding of contaminant proteins and nucleases, and increase the ability of proteinase K to neutralize unwanted proteins.

Additional resources
Characterization of thermal denaturation process of proteinase K by spectrometryEnzymesAmplite® Universal Fluorimetric Protease Activity Assay Kit *Green Fluorescence*

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