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What primer concentration should I use for PCR?
Posted February 13, 2023
Answer
The recommended primer concentration for standard PCR applications is between 0.1μM and 1μM of each primer. Generally, using a low primer concentration is better. It ensures a lower background and cleaner product. On the other hand, using a higher primer concentration may lead to nonspecific primer binding and the creation of undesirable, spurious PCR products.
Additional resources
Polymerase chain reaction : basic protocols