AAT Bioquest

What primer concentration should I use for PCR?

Posted February 13, 2023


The recommended primer concentration for standard PCR applications is between 0.1μM and 1μM of each primer. Generally, using a low primer concentration is better. It ensures a lower background and cleaner product. On the other hand, using a higher primer concentration may lead to nonspecific primer binding and the creation of undesirable, spurious PCR products.

Additional resources

Polymerase chain reaction : basic protocols

Polymerase Chain Reaction (PCR)

Helixyte™ Green *20X Aqueous PCR Solution*