What substrates are used for the ATP bioluminescence assays? | AAT Bioquest

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What substrates are used for the ATP bioluminescence assays?

Posted September 16, 2022

Chemical Reagents ATP Cellular Processes D-Luciferin Luciferase chemiluminescence firefly luciferase luciferin luminescense

Answer

ATP bioluminescence assays are based on the luciferase reaction and use the firefly luciferin substrate.

Additional resources

5,5-Dialkylluciferins are thermal stable substrates for bioluminescence-based detection systems D-Luciferin, free acid *CAS#: 2591-17-5* Luciferases

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