AAT Bioquest

When should I use isothermal amplification instead of PCR?

Posted May 20, 2023


Choosing between PCR and isothermal amplification will depend on your desired application and equipment available. 

PCR (polymerase chain reaction) is the most commonly used nucleic amplification method but it is not practical in all situations. You should use isothermal amplification instead of PCR when you don’t have access to a thermal cycler for diagnostic or point-of-care applications.

Additional resources

Isothermal Amplification of Nucleic Acids

Polymerase Chain Reaction (PCR)

Helixyte™ Green *20X Aqueous PCR Solution*