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Why are type 1 and type 3 restriction enzymes not widely used in molecular biology research?
Posted April 4, 2024
Answer
Type I restriction enzymes are not as widely used because they cleave DNA randomly and far from the recognition site, which is not practical for molecular biology research purposes. Type III restriction enzymes are also not as widely used as they cut outside the recognition sequence and rarely produce complete digests.
Additional resources
Type II restriction endonucleases—a historical perspective and more
Restriction Enzymes Cut Sites Reference Table
Amplite® Fluorimetric Coenzyme A Quantitation Kit *Green Fluorescence*