AAT Bioquest

Why is isothermal amplification not as commonly used as PCR?

Posted May 20, 2023


One reason why isothermal amplification is not as commonly used as PCR is because it is more expensive. Isothermal amplification involves multiple amplification cycles and each cycle requires a fresh polymerase containing mixture to be added to it, followed by a chemical denaturation step. This can make isothermal amplification much more expensive as compared to PCR. The higher cost acts to its disadvantage. 

Additional resources

Isothermal exponential amplification techniques: From basic principles to applications in electrochemical biosensors

Polymerase Chain Reaction (PCR)

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