AAT Bioquest


Posted on April 24, 2020
Can I use an HRP-conjugated primary antibody for western blot (with no secondary)?
Antibodies and Proteomics Bioconjugation Chemical ReagentsAntibody and Protein LabelingHRPHRP-labeled primary antibodyHorseradish Peroxidase (HRP) and Poly-HRPPrimary AntibodiesSecondary AntibodiesWestern Blotantibody conjugation

Posted on January 6, 2020
What is a Direct ELISA?
HRPTMBELISADirect ELISAcolorimetricprimary antibodyHRP-labeled primary antibody

Posted on March 28, 2019
What is a Sandwich ELISA?
ELISAprimary antibodyHRP-labeled primary antibodysecondary antibodySignal Amplificationmicroplatemicroplate readerConjugationhorseradish peroxidaseCalculating Concentration