This calculator generates an EC

How to use this tool:

1. Paste experimental data into the box on the right. Data can be copied directly from Excel columns. Data can also be comma-separated, tab-separated or space-separated values. If entering data manually, only enter one concentration per line.

Replicates can be graphed simultaneously. Graph will generated error bars based on the standard error of the mean (SEM). Simply paste or enter all data columns to begin. Format should be as follows:

Users can graph up to three data sets on the same graph for comparison purposes. To add a new data set, press the "+" tab above the data entry area. Data sets can be renamed by double clicking the tab. Each dataset will generate a corresponding EC_{50} value as well as the equation for the best fit line.

2. Verify your data is accurate in the table that appears.

3. Press the "Calculate EC_{50}" button to display results, including calculations and graph.

1. Paste experimental data into the box on the right. Data can be copied directly from Excel columns. Data can also be comma-separated, tab-separated or space-separated values. If entering data manually, only enter one concentration per line.

Replicates can be graphed simultaneously. Graph will generated error bars based on the standard error of the mean (SEM). Simply paste or enter all data columns to begin. Format should be as follows:

Concentration | Response 1 | Response 2 | ... |

C_{1} | R1_{1} | R2_{1} | ... |

C_{2} | R1_{2} | R2_{2} | ... |

C_{3} | R1_{3} | R2_{3} | ... |

... | ... | ... | ... |

Users can graph up to three data sets on the same graph for comparison purposes. To add a new data set, press the "+" tab above the data entry area. Data sets can be renamed by double clicking the tab. Each dataset will generate a corresponding EC

2. Verify your data is accurate in the table that appears.

3. Press the "Calculate EC

Process Data

References

This online tool may be cited as follows | |

MLA | "Quest Graph™ EC50 Calculator." AAT Bioquest, Inc, 21 Aug. 2019, https://www.aatbio.com/tools/ec50-calculator. |

APA | AAT Bioquest, Inc. (2019, August 21). Quest Graph™ EC50 Calculator.". Retrieved from https://www.aatbio.com/tools/ec50-calculator |

This online tool has been cited in the following publications | |

The cardenolides strophanthidin, digoxigenin and dihydroouabain act as activators of the human RORγ/RORγT receptors Authors: Karas, Kaja ; Salkowska, Anna ; Walczak-Drzewiecka, Aurelia ; Ryba, Katarzyna ; Dastych, Jaroslaw ; Bachorz, Rafal A ; Ratajewski, Marcin Journal: Toxicology letters (2018) | |

Biotransformation of estrone, 17β-estradiol and 17α-ethynylestradiol by four species of microalgae Authors: Wang, Yuwen ; Sun, Qian ; Li, Yan ; Wang, Hongjie ; Wu, Kun ; Yu, Chang-Ping Journal: Ecotoxicology and environmental safety (2019) | |