AAT Bioquest

EC50 Calculator

EC50 represents the concentration at which a substance exerts half of its maximal response. This value is particularly important in pharmacology, because it serves as an indication of drug potency. Traditionally, EC50 is expressed as a molar concentration.

This calculator generates an EC50 value typically thought of as the relative EC50. For technical assistance on using this calculator, please contact websupport@aatbio.com.

This tool has recently been updated.

How to use this tool

1. Paste experimental data into the box on the right. Data can be copied directly from Excel columns. Data can also be comma-separated, tab-separated or space-separated values. If entering data manually, only enter one X-Value per line.

Replicates can be graphed simultaneously. Graph will generate error bars based on the standard error of the mean (SEM). Simply paste or enter all data columns to begin. Format should be as follows:

ConcentrationResponse 1Response 2...

Users can graph up to three data sets on the same graph for comparison purposes. To add a new data set , press the "+" tab above the data entry area.Data sets can be renamed by double clicking the tab. Each dataset will generate a corresponding EC50 value as well as the equation for the best fit line.

2. Verify your data is accurate in the table that appears.

3. Press the "Calculate EC50" button to display results, including calculations and graph.

Data Entry


Process data


Have a question or a feature request about this tool? Feel free to reach out to us and let us know! We're always looking for ways to improve!

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This online tool may be cited as follows


"Quest Graph™ EC50 Calculator." AAT Bioquest, Inc.27 Mar2023https://www.aatbio.com/tools/ec50-calculator.


AAT Bioquest, Inc. (2023March 27). Quest Graph™ EC50 Calculator. AAT Bioquest. https://www.aatbio.com/tools/ec50-calculator.

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