Calculator
ED50 Calculator
ED50 (or "median effective dose") represents the dose at which a substance produces a therapeutic or desired effect in half of the members of a tested population. The therapeutic effect is a binary measurement, meaning either the desired effect occurs or it does not. In pharmacology, the ED50 value is important because it serves as a rough guideline for the efficacy of a drug. It differs from the EC50 of a substance, though the two sound similar. Namely, the EC50 represents the dose at which a substance produces half of its maximal effect whereas the ED50 represents the dose at which a substance produces an effect in half of the population.
For technical assistance on using this calculator, please contact websupport@aatbio.com.
For technical assistance on using this calculator, please contact websupport@aatbio.com.
How to use this tool
1. Paste experimental data into the box on the right. Data can be copied directly from Excel columns. Data can also be comma-separated, tab-separated or space-separated values. If entering data manually, only enter one X-Value per line.
Replicates can be graphed simultaneously. Graph will generate error bars based on the standard error of the mean (SEM). Simply paste or enter all data columns to begin. Format should be as follows:
Users can graph up to three data sets on the same graph for comparison purposes. To add a new data set , press the "+" tab above the data entry area.Data sets can be renamed by double clicking the tab. Each dataset will generate a corresponding ED50 value as well as the equation for the best fit line.
2. Verify your data is accurate in the table that appears.
3. Press the "Calculate ED50" button to display results, including calculations and graph.
Replicates can be graphed simultaneously. Graph will generate error bars based on the standard error of the mean (SEM). Simply paste or enter all data columns to begin. Format should be as follows:
| Concentration | Response 1 | Response 2 | ... |
| C1 | R11 | R21 | ... |
| C2 | R12 | R22 | ... |
| C3 | R13 | R23 | ... |
| ... | ... | ... | ... |
Users can graph up to three data sets on the same graph for comparison purposes. To add a new data set , press the "+" tab above the data entry area.Data sets can be renamed by double clicking the tab. Each dataset will generate a corresponding ED50 value as well as the equation for the best fit line.
2. Verify your data is accurate in the table that appears.
3. Press the "Calculate ED50" button to display results, including calculations and graph.
Data Entry
+
Feedback
Have a question or a feature request about this tool? Feel free to reach out to us and let us know! We're always looking for ways to improve!References
This online tool may be cited as follows
MLA | "Quest Graph™ ED50 Calculator." AAT Bioquest, Inc., 3 Sep. 2025, https://www.aatbio.com/tools/ed50-calculator. | |
APA | AAT Bioquest, Inc. (2025, September 3). Quest Graph™ ED50 Calculator. AAT Bioquest. https://www.aatbio.com/tools/ed50-calculator. | |
| BibTeX | EndNote | RefMan |
This online tool has been cited in 13 publications, including
Naja nigricincta nigricincta venom, a murine model. Evaluation of skeletal and cardio-myonecrosis, kidney injury and inflammatory response along with neutralisation efficacy by the SAIMR/SAVP-and EchiTAb-Plus-ICP polyvalent antivenoms.
Authors: Saaiman, Esta L and Naidoo, Vinny and Botha, Christo J
Journal: Toxicon (2024): 107719
Authors: Saaiman, Esta L and Naidoo, Vinny and Botha, Christo J
Journal: Toxicon (2024): 107719
Intrauterine Transmission of Zika and Vertical Transfer of Neutralizing Antibodies Detected Immediately at Birth in Oaxaca, Mexico: An Analysis in the Context of Microcephaly
Authors: Porras-Garc{\'\i}a, Alfredo and Villanueva-Garc{\'\i}a, Dina and Arnaud-Rios, Rafael and Garc{\'\i}a-Lemus, Nadia and Castillo-Romero, Ang{\'e}lica and Mej{\'\i}a-Flores, Mariana and Contreras, Luis Erik and Hern{\'a}ndez-Castillo, Liliana and Jim{\'e}nez-Hern{\'a}ndez, Elva and Mej{\'\i}a-Arangur{\'e}, Juan Manuel and others,
Journal: Microorganisms (2024): 423
Authors: Porras-Garc{\'\i}a, Alfredo and Villanueva-Garc{\'\i}a, Dina and Arnaud-Rios, Rafael and Garc{\'\i}a-Lemus, Nadia and Castillo-Romero, Ang{\'e}lica and Mej{\'\i}a-Flores, Mariana and Contreras, Luis Erik and Hern{\'a}ndez-Castillo, Liliana and Jim{\'e}nez-Hern{\'a}ndez, Elva and Mej{\'\i}a-Arangur{\'e}, Juan Manuel and others,
Journal: Microorganisms (2024): 423
Antiplasmodial Activity of Ethanol Extract of Sonneratia alba Leaves.
Authors: Muhaimin, Muhaimin and Latifah, Nor and Chaerunisaa, Anis Y and Subarnas, Anas and Susilawati, Yasmiwar and Hirzan, Rifanza
Journal: Tropical Journal of Natural Product Research (2024)
Authors: Muhaimin, Muhaimin and Latifah, Nor and Chaerunisaa, Anis Y and Subarnas, Anas and Susilawati, Yasmiwar and Hirzan, Rifanza
Journal: Tropical Journal of Natural Product Research (2024)
Phytochemical Analysis, $\alpha$-Glucosidase and $\alpha$-Amylase Inhibitory Activities and Acute Toxicity Studies of Extracts from Pomegranate (Punica granatum) Bark, a Valuable Agro-Industrial By-Product. Foods 2022, 11, 1353
Authors: Laaraj, N and Bouhrim, M and Kharchoufa, L and Tiji, S and Bendaha, H and Addi, M and Drouet, S and Hano, C and Lorenzo, JM and Bnouham, M and others,
Journal: (2022)
Authors: Laaraj, N and Bouhrim, M and Kharchoufa, L and Tiji, S and Bendaha, H and Addi, M and Drouet, S and Hano, C and Lorenzo, JM and Bnouham, M and others,
Journal: (2022)
Introducing a New Method for Purification of Human IL-4 by Substitution of a Single Amino Acid in IL-4 Protein Sequence
Authors: Mazloomrezaei, Mohsen and Hosseini, Mahsa Sadat and Ahmadi, Nahid and Mahmoudi Maymand, Elham and Eftekhar, Ebrahim and Asgari, Amir and Ramezani, Amin
Journal: Iranian Journal of Immunology (2022): 436--445
Authors: Mazloomrezaei, Mohsen and Hosseini, Mahsa Sadat and Ahmadi, Nahid and Mahmoudi Maymand, Elham and Eftekhar, Ebrahim and Asgari, Amir and Ramezani, Amin
Journal: Iranian Journal of Immunology (2022): 436--445
Prophylactic, Suppressive and Curative Potentials of a Polyherbal Antimalaria Mixture in Plasmodium Berghei Infected Mice
Authors: Mikailu, Suleiman and Ledumbari, Vigara Sophia and Stephen, Nzereogu Chibuike and Nnenna, Doris and Ajibo, Afieroho Ozadheoghene Eriarie and Abo, Kio Anthony
Journal: (2021)
Authors: Mikailu, Suleiman and Ledumbari, Vigara Sophia and Stephen, Nzereogu Chibuike and Nnenna, Doris and Ajibo, Afieroho Ozadheoghene Eriarie and Abo, Kio Anthony
Journal: (2021)
High cytotoxicity of betulin towards fish and murine fibroblasts: Is betulin safe for nonneoplastic cells?
Authors: Ma{\l}aczewska, Joanna and Kaczorek-{\L}ukowska, Edyta and Kazu{\'n}, Barbara
Journal: BMC veterinary research (2021): 1--13
Authors: Ma{\l}aczewska, Joanna and Kaczorek-{\L}ukowska, Edyta and Kazu{\'n}, Barbara
Journal: BMC veterinary research (2021): 1--13
Genome-wide Screening of Genes Associated with Momilactone B Sensitivity in the Fission Yeast
Authors: Tomita, Keisuke and Yashiroda, Yoko and Matsuo, Yasuhiro and Piotrowski, Jeff S and Li, Sheena C and Okamoto, Reika and Yoshimura, Mami and Kimura, Hiromi and Kawamura, Yumi and Kawamukai, Makoto and others,
Journal: G3 Genes| Genomes| Genetics (2021)
Authors: Tomita, Keisuke and Yashiroda, Yoko and Matsuo, Yasuhiro and Piotrowski, Jeff S and Li, Sheena C and Okamoto, Reika and Yoshimura, Mami and Kimura, Hiromi and Kawamura, Yumi and Kawamukai, Makoto and others,
Journal: G3 Genes| Genomes| Genetics (2021)
Design, synthesis and biological evaluation of naphthalene-derived (arylalkyl) azoles containing heterocyclic linkers as new anticonvulsants: a comprehensive in silico, in vitro, and in vivo study
Authors: Valipour, Mehdi and Naderi, Nima and Heidarli, Elmira and Shaki, Fatemeh and Motafeghi, Farzaneh Sadat and Amiri, Fereshteh Talebpour and Emami, Saeed and Irannejad, Hamid
Journal: European Journal of Pharmaceutical Sciences (2021): 105974
Authors: Valipour, Mehdi and Naderi, Nima and Heidarli, Elmira and Shaki, Fatemeh and Motafeghi, Farzaneh Sadat and Amiri, Fereshteh Talebpour and Emami, Saeed and Irannejad, Hamid
Journal: European Journal of Pharmaceutical Sciences (2021): 105974
The toxic influence of silver and titanium dioxide nanoparticles on cultured ovarian granulosa cells
Authors: Sirotkin, Alexander V and Bauer, Miroslav and Kadasi, Attila and Makovicky, Pavol and Scsukova, Sona
Journal: Reproductive Biology (2020): 100467
Authors: Sirotkin, Alexander V and Bauer, Miroslav and Kadasi, Attila and Makovicky, Pavol and Scsukova, Sona
Journal: Reproductive Biology (2020): 100467
Substrate Specific Inhibitor Designed against the Immunomodulator GMF-beta Reversed the Experimental Autoimmune Encephalomyelitis
Authors: Vattathara, Jane Jose and Prakash, Ohm and Subhramanian, Sunitha and Satheeshkumar, Madathiparambil Kumaran and Xavier, Tessy and Anil, Meenakshi and Pillai, Gopal S and Anandakuttan, Anandkumar and Radhakrishnan, Sureshkumar and Sivanarayanan, TB and others,
Journal: Scientific reports (2020): 1--13
Authors: Vattathara, Jane Jose and Prakash, Ohm and Subhramanian, Sunitha and Satheeshkumar, Madathiparambil Kumaran and Xavier, Tessy and Anil, Meenakshi and Pillai, Gopal S and Anandakuttan, Anandkumar and Radhakrishnan, Sureshkumar and Sivanarayanan, TB and others,
Journal: Scientific reports (2020): 1--13
Synthesis of Pyrrolo [3, 4-b] pyridin-5-ones via Multicomponent Reactions and In Vitro--In Silico Studies Against SiHa, HeLa, and CaSki Human Cervical Carcinoma Cell Lines
Authors: Segura-Olvera, Daniel and Garc{\'\i}a-Gonz{\'a}lez, Ailyn N and Morales-Salazar, Ivette and Islas-J{\'a}come, Alejandro and Rojas-Aguirre, Yareli and Ibarra, Ilich A and D{\'\i}az-Cervantes, Erik and Alcaraz-Estrada, Sof{\'\i}a Lizeth and Gonz{\'a}lez-Zamora, Eduardo
Journal: Molecules (2019): 2648
Authors: Segura-Olvera, Daniel and Garc{\'\i}a-Gonz{\'a}lez, Ailyn N and Morales-Salazar, Ivette and Islas-J{\'a}come, Alejandro and Rojas-Aguirre, Yareli and Ibarra, Ilich A and D{\'\i}az-Cervantes, Erik and Alcaraz-Estrada, Sof{\'\i}a Lizeth and Gonz{\'a}lez-Zamora, Eduardo
Journal: Molecules (2019): 2648
Alternative Treatment Options for Controlling Anthelmintic-Resistant Haemonchus contortus Populations in South Dakota Sheep Herds
Authors: Sarah, Adam
Journal: (2019)
Authors: Sarah, Adam
Journal: (2019)