Calculator
ED50 Calculator
ED50 (or "median effective dose") represents the dose at which a substance produces a therapeutic or desired effect in half of the members of a tested population. The therapeutic effect is a binary measurement, meaning either the desired effect occurs or it does not. In pharmacology, the ED50 value is important because it serves as a rough guideline for the efficacy of a drug. It differs from the EC50 of a substance, though the two sound similar. Namely, the EC50 represents the dose at which a substance produces half of its maximal effect whereas the ED50 represents the dose at which a substance produces an effect in half of the population.
For technical assistance on using this calculator, please contact websupport@aatbio.com.
For technical assistance on using this calculator, please contact websupport@aatbio.com.
How to use this tool
1. Paste experimental data into the box on the right. Data can be copied directly from Excel columns. Data can also be comma-separated, tab-separated or space-separated values. If entering data manually, only enter one X-Value per line.
Replicates can be graphed simultaneously. Graph will generate error bars based on the standard error of the mean (SEM). Simply paste or enter all data columns to begin. Format should be as follows:
Users can graph up to three data sets on the same graph for comparison purposes. To add a new data set , press the "+" tab above the data entry area.Data sets can be renamed by double clicking the tab. Each dataset will generate a corresponding ED50 value as well as the equation for the best fit line.
2. Verify your data is accurate in the table that appears.
3. Press the "Calculate ED50" button to display results, including calculations and graph.
Replicates can be graphed simultaneously. Graph will generate error bars based on the standard error of the mean (SEM). Simply paste or enter all data columns to begin. Format should be as follows:
| Concentration | Response 1 | Response 2 | ... |
| C1 | R11 | R21 | ... |
| C2 | R12 | R22 | ... |
| C3 | R13 | R23 | ... |
| ... | ... | ... | ... |
Users can graph up to three data sets on the same graph for comparison purposes. To add a new data set , press the "+" tab above the data entry area.Data sets can be renamed by double clicking the tab. Each dataset will generate a corresponding ED50 value as well as the equation for the best fit line.
2. Verify your data is accurate in the table that appears.
3. Press the "Calculate ED50" button to display results, including calculations and graph.
Data Entry
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Have a question or a feature request about this tool? Feel free to reach out to us and let us know! We're always looking for ways to improve!References
This online tool may be cited as follows
MLA | "Quest Graph™ ED50 Calculator." AAT Bioquest, Inc., 30 Apr. 2024, https://www.aatbio.com/tools/ed50-calculator. | |
APA | AAT Bioquest, Inc. (2024, April 30). Quest Graph™ ED50 Calculator. AAT Bioquest. https://www.aatbio.com/tools/ed50-calculator. | |
| BibTeX | EndNote | RefMan |
This online tool has been cited in 12 publications, including
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Phytochemical Analysis, $\alpha$-Glucosidase and $\alpha$-Amylase Inhibitory Activities and Acute Toxicity Studies of Extracts from Pomegranate (Punica granatum) Bark, a Valuable Agro-Industrial By-Product. Foods 2022, 11, 1353
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High cytotoxicity of betulin towards fish and murine fibroblasts: Is betulin safe for nonneoplastic cells?
Authors: Ma{\l}aczewska, Joanna and Kaczorek-{\L}ukowska, Edyta and Kazu{\'n}, Barbara
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Authors: Tomita, Keisuke and Yashiroda, Yoko and Matsuo, Yasuhiro and Piotrowski, Jeff S and Li, Sheena C and Okamoto, Reika and Yoshimura, Mami and Kimura, Hiromi and Kawamura, Yumi and Kawamukai, Makoto and others,
Journal: G3 Genes| Genomes| Genetics (2021)
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Journal: European Journal of Pharmaceutical Sciences (2021): 105974
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Journal: European Journal of Pharmaceutical Sciences (2021): 105974
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Authors: Sirotkin, Alexander V and Bauer, Miroslav and Kadasi, Attila and Makovicky, Pavol and Scsukova, Sona
Journal: Reproductive Biology (2020): 100467
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Journal: Reproductive Biology (2020): 100467
Substrate Specific Inhibitor Designed against the Immunomodulator GMF-beta Reversed the Experimental Autoimmune Encephalomyelitis
Authors: Vattathara, Jane Jose and Prakash, Ohm and Subhramanian, Sunitha and Satheeshkumar, Madathiparambil Kumaran and Xavier, Tessy and Anil, Meenakshi and Pillai, Gopal S and Anandakuttan, Anandkumar and Radhakrishnan, Sureshkumar and Sivanarayanan, TB and others,
Journal: Scientific reports (2020): 1--13
Authors: Vattathara, Jane Jose and Prakash, Ohm and Subhramanian, Sunitha and Satheeshkumar, Madathiparambil Kumaran and Xavier, Tessy and Anil, Meenakshi and Pillai, Gopal S and Anandakuttan, Anandkumar and Radhakrishnan, Sureshkumar and Sivanarayanan, TB and others,
Journal: Scientific reports (2020): 1--13
Synthesis of Pyrrolo [3, 4-b] pyridin-5-ones via Multicomponent Reactions and In Vitro--In Silico Studies Against SiHa, HeLa, and CaSki Human Cervical Carcinoma Cell Lines
Authors: Segura-Olvera, Daniel and Garc{\'\i}a-Gonz{\'a}lez, Ailyn N and Morales-Salazar, Ivette and Islas-J{\'a}come, Alejandro and Rojas-Aguirre, Yareli and Ibarra, Ilich A and D{\'\i}az-Cervantes, Erik and Alcaraz-Estrada, Sof{\'\i}a Lizeth and Gonz{\'a}lez-Zamora, Eduardo
Journal: Molecules (2019): 2648
Authors: Segura-Olvera, Daniel and Garc{\'\i}a-Gonz{\'a}lez, Ailyn N and Morales-Salazar, Ivette and Islas-J{\'a}come, Alejandro and Rojas-Aguirre, Yareli and Ibarra, Ilich A and D{\'\i}az-Cervantes, Erik and Alcaraz-Estrada, Sof{\'\i}a Lizeth and Gonz{\'a}lez-Zamora, Eduardo
Journal: Molecules (2019): 2648
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