Benzoyl-Leu-Gly-Arg-Aminoluciferin
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 823.86 |
Solvent | DMSO |
Spectral properties
Extinction coefficient (cm -1 M -1) | 18000 |
Excitation (nm) | 362 |
Emission (nm) | 499 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
See also: Proteases
Molecular weight 823.86 | Extinction coefficient (cm -1 M -1) 18000 | Excitation (nm) 362 | Emission (nm) 499 |
This aminoluciferin-based probe is a luminogenic substrate for complement component C3/C5 convertases, coagulation factor Xa, and soybean trypsin-like enzyme. It is also hydrolyzed by macropain, a high molecular weight thiol proteinase from human erythrocytes. The protease-released D-aminoluciferin can be detected using our luciferase assay kits. Benzoyl-Leu-Gly-Arg-Aminoluciferin is much more sensitive than Boc-Leu-Gly-Arg-AMC, the widely used fluorogenic AMC substrate.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Benzoyl-Leu-Gly-Arg-Aminoluciferin to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 121.38 µL | 606.899 µL | 1.214 mL | 6.069 mL | 12.138 mL |
5 mM | 24.276 µL | 121.38 µL | 242.76 µL | 1.214 mL | 2.428 mL |
10 mM | 12.138 µL | 60.69 µL | 121.38 µL | 606.899 µL | 1.214 mL |
Molarity calculator
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Extinction coefficient (cm -1 M -1) | 18000 |
Excitation (nm) | 362 |
Emission (nm) | 499 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
BOC-Leu-Gly-Arg-Aminoluciferin | 362 | 499 | 18000 |
Citations
View all 2 citations: Citation Explorer
Improved bioluminescence-based endotoxin measurement method using a salt-resistant luciferase mutant
Authors: Yawata, Satoshi and Noda, Kenichi and Shimomura, Ai and Kuroda, Akio
Journal: Analytical Biochemistry (2021): 114408
Authors: Yawata, Satoshi and Noda, Kenichi and Shimomura, Ai and Kuroda, Akio
Journal: Analytical Biochemistry (2021): 114408
Endotoxin assay by bioluminescence using mutant firefly luciferase
Authors: Noda, Kenichi and Goto, Hitoshi and Murakami, Yuji and Ahmed, Abo Bakr F and Kuroda, Akio
Journal: Analytical biochemistry (2010): 152--155
Authors: Noda, Kenichi and Goto, Hitoshi and Murakami, Yuji and Ahmed, Abo Bakr F and Kuroda, Akio
Journal: Analytical biochemistry (2010): 152--155
References
View all 16 references: Citation Explorer
Identification of mutant firefly luciferases that efficiently utilize aminoluciferins
Authors: Harwood KR, Mofford DM, Reddy GR, Miller SC.
Journal: Chem Biol (2011): 1649
Authors: Harwood KR, Mofford DM, Reddy GR, Miller SC.
Journal: Chem Biol (2011): 1649
In vivo imaging of early stage apoptosis by measuring real-time caspase-3/7 activation
Authors: Scabini M, Stellari F, Cappella P, Rizzitano S, Texido G, Pesenti E.
Journal: Apoptosis (2011): 198
Authors: Scabini M, Stellari F, Cappella P, Rizzitano S, Texido G, Pesenti E.
Journal: Apoptosis (2011): 198
Aminoluciferins as functional bioluminogenic substrates of firefly luciferase
Authors: Takakura H, Kojima R, Urano Y, Terai T, Hanaoka K, Nagano T.
Journal: Chem Asian J (2011): 1800
Authors: Takakura H, Kojima R, Urano Y, Terai T, Hanaoka K, Nagano T.
Journal: Chem Asian J (2011): 1800
Noninvasive molecular imaging of apoptosis in vivo using a modified firefly luciferase substrate, Z-DEVD-aminoluciferin
Authors: Hickson J, Ackler S, Klaubert D, Bouska J, Ellis P, Foster K, Oleksijew A, Rodriguez L, Schlessinger S, Wang B, Frost D.
Journal: Cell Death Differ (2010): 1003
Authors: Hickson J, Ackler S, Klaubert D, Bouska J, Ellis P, Foster K, Oleksijew A, Rodriguez L, Schlessinger S, Wang B, Frost D.
Journal: Cell Death Differ (2010): 1003
Robust light emission from cyclic alkylaminoluciferin substrates for firefly luciferase
Authors: Reddy, G. R.; Thompson, W. C.; Miller, S. C.
Journal: J Am Chem Soc (2010): 13586-7
Authors: Reddy, G. R.; Thompson, W. C.; Miller, S. C.
Journal: J Am Chem Soc (2010): 13586-7
In vivo bioluminescence imaging of furin activity in breast cancer cells using bioluminogenic substrates
Authors: Dragulescu-Andrasi A, Liang G, Rao J.
Journal: Bioconjug Chem (2009): 1660
Authors: Dragulescu-Andrasi A, Liang G, Rao J.
Journal: Bioconjug Chem (2009): 1660
Cell-based bioluminescent assays for all three proteasome activities in a homogeneous format
Authors: Moravec RA, O'Brien MA, Daily WJ, Scurria MA, Bernad L, Riss TL.
Journal: Anal Biochem (2009): 294
Authors: Moravec RA, O'Brien MA, Daily WJ, Scurria MA, Bernad L, Riss TL.
Journal: Anal Biochem (2009): 294
Homogeneous, bioluminescent proteasome assays
Authors: O'Brien MA, Moravec RA, Riss TL, Bulleit RF.
Journal: Methods Mol Biol (2008): 163
Authors: O'Brien MA, Moravec RA, Riss TL, Bulleit RF.
Journal: Methods Mol Biol (2008): 163
N-Alkylated 6'-aminoluciferins are bioluminescent substrates for Ultra-Glo and QuantiLum luciferase: new potential scaffolds for bioluminescent assays
Authors: Woodroofe CC, Shultz JW, Wood MG, Osterman J, Cali JJ, Daily WJ, Meisenheimer PL, Klaubert DH.
Journal: Biochemistry (2008): 10383
Authors: Woodroofe CC, Shultz JW, Wood MG, Osterman J, Cali JJ, Daily WJ, Meisenheimer PL, Klaubert DH.
Journal: Biochemistry (2008): 10383
A homogeneous assay to measure live and dead cells in the same sample by detecting different protease markers
Authors: Niles AL, Moravec RA, Eric Hesselberth P, Scurria MA, Daily WJ, Riss TL.
Journal: Anal Biochem (2007): 197
Authors: Niles AL, Moravec RA, Eric Hesselberth P, Scurria MA, Daily WJ, Riss TL.
Journal: Anal Biochem (2007): 197
Application notes
Abbreviation of Common Chemical Compounds Related to Peptides
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An Increase in Plasma Homovanillic Acid with Cocoa Extract Consumption Is Associated with the Alleviation of Depressive Symptoms in Overweight or Obese Adults
Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severity
β-adrenoceptors are upregulated in human melanoma and their activation releases pro-tumorigenic cytokines and metalloproteases in melanoma cell lines
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
An Increase in Plasma Homovanillic Acid with Cocoa Extract Consumption Is Associated with the Alleviation of Depressive Symptoms in Overweight or Obese Adults
Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severity
β-adrenoceptors are upregulated in human melanoma and their activation releases pro-tumorigenic cytokines and metalloproteases in melanoma cell lines