CytoCalcein™ Violet 660, sodium salt Excited at 405 nm

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	662.63
Solvent	DMSO

Spectral properties

Excitation (nm)	402
Emission (nm)	660

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Molecular weight

662.63

Excitation (nm)

402

Emission (nm)

660

CytoCalcein™ Violet 660 is the esterase-cleaved product of CytoCalcein™ Violet 660 AM in live cells. CytoCalcein™ Violet 660 is not cell-permeable. It is the reference product for the CytoCalcein™ Violet 660 AM that is designed for labeling live cells in the same way as Calcein, AM does. CytoCalcein™ Violet 660 is well excited with the Violet laser at 405 nm, which is now equipped in most flow cytometers. It is also well-excited by the excitation sources of fluorescence microscopes. This exceptional spectral separation from the typical existing FACS fluorophores provides additional options for multiplexing experiments with a flow cytometer or a fluorescence microscope. CytoCalcein™ Violet 660 has been developed for multiplexing flow cytometric and fluorescence imaging applications. It provides a new unique color for flow cytometric analysis of live cells.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of CytoCalcein™ Violet 660, sodium salt *Excited at 405 nm* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	150.914 µL	754.569 µL	1.509 mL	7.546 mL	15.091 mL
5 mM	30.183 µL	150.914 µL	301.828 µL	1.509 mL	3.018 mL
10 mM	15.091 µL	75.457 µL	150.914 µL	754.569 µL	1.509 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)	402
Emission (nm)	660

References

View all 50 references: Citation Explorer

A Ratiometric Probe Based on Carbon Dots and Calcein & Eu3+ for the Fluorescent Detection of Sodium Tripolyphosphate.
Authors: Zheng, Yabin and Li, Chen and Li, Quan and Zhang, Taoyi and Chen, Jing and Ji, Wenxi and Wei, Yun
Journal: Journal of fluorescence (2023): 965-972

Dual-emission fluorescence detection of histidine using carbon dots and calcein/Ni2+ complexes.
Authors: Liu, Jinshui and Song, Bo and Fu, Ting
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2023): 121951

Construction of N-CDs and Calcein-Based Ratiometric Fluorescent Sensor for Rapid Detection of Arginine and Acetaminophen.
Authors: Qi, Haiyan and Li, Qiuying and Jing, Jing and Jing, Tao and Liu, Chuntong and Qiu, Lixin and Sami, Rokayya and Helal, Mahmoud and Ismail, Khadiga Ahmed and Aljahani, Amani H
Journal: Nanomaterials (Basel, Switzerland) (2022)

Tracking the Endosomal Escape: A Closer Look at Calcein and Related Reporters.
Authors: Hausig-Punke, Franziska and Richter, Friederike and Hoernke, Maria and Brendel, Johannes C and Traeger, Anja
Journal: Macromolecular bioscience (2022): e2200167

Sensitive ratiometric fluorescence probe based on chitosan carbon dots and calcein for Alkaline phosphatase detection and bioimaging in cancer cells.
Authors: Mu, Xiaowei and Jiang, Xue and Zhang, Yu and Liu, Xin and Zhang, Siqi and Wang, Wei and Huang, Yibing and Ma, Pinyi and Song, Daqian
Journal: Analytica chimica acta (2021): 339163

Calcein Binding to Assess Mineralization in Hydrogel Microspheres.
Authors: White, Kristopher and Chalaby, Rabab and Lowe, Gina and Berlin, Jacob and Glackin, Carlotta and Olabisi, Ronke
Journal: Polymers (2021)

Evaluation of NK cell cytotoxic activity against malignant cells by the calcein assay.
Authors: Lorenzo-Herrero, Seila and Sordo-Bahamonde, Christian and González, Segundo and López-Soto, Alejandro
Journal: Methods in enzymology (2020): 483-495

Calcein Fluorescence Quenching to Measure Plasma Membrane Water Flux in Live Mammalian Cells.
Authors: Kitchen, Philip and Salman, Mootaz M and Abir-Awan, Mohammed and Al-Jubair, Tamim and Törnroth-Horsefield, Susanna and Conner, Alex C and Bill, Roslyn M
Journal: STAR protocols (2020): 100157

Calcein Release Assay to Measure Membrane Permeabilization by Recombinant Alpha-Synuclein.
Authors: Dutta, Sayan and Watson, Ben G and Mattoo, Seema and Rochet, Jean-Christophe
Journal: Bio-protocol (2020)

The Relationship Between Water Transmission Rate and Defects on the Film Based on the Defect Analysis Using Fluorescent Calcein Probe.
Authors: Kim, Sun Min and Kim, Hyun Chul and Kim, Chang-Yeoul and Yoon, Daeho and Koo, Eunhae
Journal: Journal of nanoscience and nanotechnology (2020): 5469-5472