CytoWatch™ Multiplexing Buffer for Flow Cytometry *20X*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Solvent | Water |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Buffers and Lab Consumables
Fluorescent antibody conjugates tend to aggregate when in close contact, distorting the analytical results of multicolor staining cells due to FRET and other energy transfer phenomena between different conjugates. Fluorescent dye interactions may cause staining artifacts, complicating the data interpretation. CytoWatch™ Multiplexing Buffer is a buffer optimized for the multicolor flow cytometric analysis of cells. CytoWatch™ Multiplexing Buffer is a concentrated solution that is directly added to the staining solutions of certain fluorescent antibody conjugates or other fluorescent reagents before staining cells. It might be used to enhance or improve multicolor flow cytometry experiments that utilize two or more different staining reagents (e.g., antibodies conjugated with BV dyes or Qdots).
Example protocol
SAMPLE EXPERIMENTAL PROTOCOL
Cell Surface Staining Protocol for Flow Cytometry Analysis:
- Add 5 µL of CytoWatch™ Multiplexing Buffer to 100 µL of PBMC. Mix well by pipetting up and down several times.
- Add appropriate amounts of conjugated antibodies.
- Mix well by pipetting up and down and stain at room temperature or 2~8°C for 20 minutes.
- Wash twice with a flow cytometric cell staining buffer of your choice.
- Resuspend cells in an appropriate volume of flow cytometric cell staining buffer.
- Perform flow cytometric analysis.
References
View all 46 references: Citation Explorer
Azo dyes decolorization under high alkalinity and salinity conditions by Halomonas sp. in batch and packed bed reactor.
Authors: Montañez-Barragán, B and Sanz-Martín, J L and Gutiérrez-Macías, P and Morato-Cerro, A and Rodríguez-Vázquez, R and Barragán-Huerta, B E
Journal: Extremophiles : life under extreme conditions (2020): 239-247
Authors: Montañez-Barragán, B and Sanz-Martín, J L and Gutiérrez-Macías, P and Morato-Cerro, A and Rodríguez-Vázquez, R and Barragán-Huerta, B E
Journal: Extremophiles : life under extreme conditions (2020): 239-247
Adsorption of Remazol Brilliant Violet-5R Textile Dye from Aqueous Solutions by Using Eggshell Waste Biosorbent.
Authors: Rápó, Eszter and Aradi, László Előd and Szabó, Ábel and Posta, Katalin and Szép, Robert and Tonk, Szende
Journal: Scientific reports (2020): 8385
Authors: Rápó, Eszter and Aradi, László Előd and Szabó, Ábel and Posta, Katalin and Szép, Robert and Tonk, Szende
Journal: Scientific reports (2020): 8385
Process optimization and filtration performance of an anaerobic dynamic membrane bioreactor treating textile wastewaters.
Authors: Yurtsever, Adem and Basaran, Erkan and Ucar, Deniz
Journal: Journal of environmental management (2020): 111114
Authors: Yurtsever, Adem and Basaran, Erkan and Ucar, Deniz
Journal: Journal of environmental management (2020): 111114
Simultaneous Polychromatic Immunofluorescent Staining of Tissue Sections and Consecutive Imaging of up to Seven Parameters by Standard Confocal Microscopy.
Authors: Schmidt, Alfonso J and Mayer, Johannes U and Wallace, Paul K and Ronchese, Franca and Price, Kylie M
Journal: Current protocols in cytometry (2019): e64
Authors: Schmidt, Alfonso J and Mayer, Johannes U and Wallace, Paul K and Ronchese, Franca and Price, Kylie M
Journal: Current protocols in cytometry (2019): e64
Remediation of complex remazol effluent using biochar derived from green seaweed biomass.
Authors: Gokulan, Ravindiran and Prabhu, Ganapathy Ganesh and Jegan, Josephraj
Journal: International journal of phytoremediation (2019): 1179-1189
Authors: Gokulan, Ravindiran and Prabhu, Ganapathy Ganesh and Jegan, Josephraj
Journal: International journal of phytoremediation (2019): 1179-1189
Diet-induced microbial autofluorescence confounds flow cytometry of ex vivo isolated fecal microbes.
Authors: Denu, Lidiya and Lubin, Jean-Bernard and Douglas, Bonnie and Tuluc, Florin and Silverman, Michael A
Journal: European journal of immunology (2019): 2252-2254
Authors: Denu, Lidiya and Lubin, Jean-Bernard and Douglas, Bonnie and Tuluc, Florin and Silverman, Michael A
Journal: European journal of immunology (2019): 2252-2254
Fluorochrome choices for multi-color flow cytometry.
Authors: Flores-Montero, Juan and Kalina, Tomas and Corral-Mateos, Alba and Sanoja-Flores, Luzalba and Pérez-Andrés, Martin and Martin-Ayuso, Marta and Sedek, Lukasz and Rejlova, Katerina and Mayado, Andrea and Fernández, Paula and van der Velden, Vincent and Bottcher, Sebastian and van Dongen, Jaques J M and Orfao, Alberto
Journal: Journal of immunological methods (2019): 112618
Authors: Flores-Montero, Juan and Kalina, Tomas and Corral-Mateos, Alba and Sanoja-Flores, Luzalba and Pérez-Andrés, Martin and Martin-Ayuso, Marta and Sedek, Lukasz and Rejlova, Katerina and Mayado, Andrea and Fernández, Paula and van der Velden, Vincent and Bottcher, Sebastian and van Dongen, Jaques J M and Orfao, Alberto
Journal: Journal of immunological methods (2019): 112618
Adsorptive Removal of Remazol Brilliant Violet-5R Dye from Aqueous Solutions using Calcined Eggshell as Biosorbent.
Authors: Rápó, Eszter and Posta, Katalin and Suciu, Maria and Szép, Robert and Tonk, Szende
Journal: Acta chimica Slovenica (2019): 648-658
Authors: Rápó, Eszter and Posta, Katalin and Suciu, Maria and Szép, Robert and Tonk, Szende
Journal: Acta chimica Slovenica (2019): 648-658
Semiconductor Nanoplatelets: A New Class of Ultrabright Fluorescent Probes for Cytometric and Imaging Applications.
Authors: Kechkeche, Djamila and Cao, Edgar and Grazon, Chloé and Caschera, Filippo and Noireaux, Vincent and Baron Niel, Marie-Laurence and Dubertret, Benoit
Journal: ACS applied materials & interfaces (2018): 24739-24749
Authors: Kechkeche, Djamila and Cao, Edgar and Grazon, Chloé and Caschera, Filippo and Noireaux, Vincent and Baron Niel, Marie-Laurence and Dubertret, Benoit
Journal: ACS applied materials & interfaces (2018): 24739-24749
Adsorption of basic and reactive dyes from aqueous solution onto Intsia bijuga sawdust-based activated carbon: batch and column study.
Authors: Khasri, Azduwin and Ahmad, Mohd Azmier
Journal: Environmental science and pollution research international (2018): 31508-31519
Authors: Khasri, Azduwin and Ahmad, Mohd Azmier
Journal: Environmental science and pollution research international (2018): 31508-31519
Application notes
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What does an assay buffer do?
How to lyse cells?
Why is my GSH concentration greater than my Total GSH concentration? Why is my calculated GSH/TGSH ratio greater than 1?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?
What does an assay buffer do?
How to lyse cells?
Why is my GSH concentration greater than my Total GSH concentration? Why is my calculated GSH/TGSH ratio greater than 1?