D-Luciferin methyl ester *CAS 73918-26-0*
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Physical properties
Molecular weight | 294.35 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 385 |
Emission (nm) | 529 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 41105331 |
Overview | SDSProtocol |
See also: Luciferases, Oxidases
CAS 73918-26-0 | Molecular weight 294.35 | Excitation (nm) 385 | Emission (nm) 529 |
D-Luciferin methyl ester contains a methyl moiety attached at the carboxy position, thus preventing it be recognized by the firefly luciferase enzyme. However, the ester is hydrolyzed effectively by lipase activity, and the resulted D-luciferin is well recognized by luciferase. D-Luciferin methyl ester is a sensitive substrate for the chemiluminescent measurement of lipase activity in homogeneous assays, or in cell lysate samples in combination with luciferase and its cofactors.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of D-Luciferin methyl ester *CAS 73918-26-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 339.732 µL | 1.699 mL | 3.397 mL | 16.987 mL | 33.973 mL |
5 mM | 67.946 µL | 339.732 µL | 679.463 µL | 3.397 mL | 6.795 mL |
10 mM | 33.973 µL | 169.866 µL | 339.732 µL | 1.699 mL | 3.397 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Citations
View all 14 citations: Citation Explorer
Bmal1 integrates circadian function and temperature sensing in the suprachiasmatic nucleus
Authors: Hoekstra, Marieke MB and Ness, Natalie and Badia-Soteras, Aina and Brancaccio, Marco
Journal: Proceedings of the National Academy of Sciences (2024): e2316646121
Authors: Hoekstra, Marieke MB and Ness, Natalie and Badia-Soteras, Aina and Brancaccio, Marco
Journal: Proceedings of the National Academy of Sciences (2024): e2316646121
GRAS family member LATERAL SUPPRESSOR regulates the initiation and morphogenesis of watermelon lateral organs
Authors: Jiang, Yanxin and Zhang, Anran and He, Wenjing and Li, Qingqing and Zhao, Bosi and Zhao, Hongjiao and Ke, Xubo and Guo, Yalu and Sun, Piaoyun and Yang, Tongwen and others,
Journal: Plant Physiology (2023): kiad445
Authors: Jiang, Yanxin and Zhang, Anran and He, Wenjing and Li, Qingqing and Zhao, Bosi and Zhao, Hongjiao and Ke, Xubo and Guo, Yalu and Sun, Piaoyun and Yang, Tongwen and others,
Journal: Plant Physiology (2023): kiad445
Design, synthesis, and biological evaluation studies of novel carboxylesterase 2 inhibitors for the treatment of irinotecan-induced delayed diarrhea
Authors: Yang, Zhongcheng and Cao, Zhijun and Wang, Wenxin and Chen, Ya and Huang, Wanqiu and Jiao, Shixuan and Chen, Siliang and Chen, Lianru and Liu, Yuxia and Mao, Jianming and others,
Journal: Bioorganic Chemistry (2023): 106625
Authors: Yang, Zhongcheng and Cao, Zhijun and Wang, Wenxin and Chen, Ya and Huang, Wanqiu and Jiao, Shixuan and Chen, Siliang and Chen, Lianru and Liu, Yuxia and Mao, Jianming and others,
Journal: Bioorganic Chemistry (2023): 106625
Diltiazem inhibits breast cancer metastasis via mediating growth differentiation factor 15 and epithelial-mesenchymal transition
Authors: Chen, Yen-Chang and Wu, Chen-Teng and Chen, Jia-Hong and Tsai, Cheng-Fang and Wu, Chen-Yun and Chang, Pei-Chun and Yeh, Wei-Lan
Journal: Oncogenesis (2022): 1--9
Authors: Chen, Yen-Chang and Wu, Chen-Teng and Chen, Jia-Hong and Tsai, Cheng-Fang and Wu, Chen-Yun and Chang, Pei-Chun and Yeh, Wei-Lan
Journal: Oncogenesis (2022): 1--9
Human carboxylesterase 1A plays a predominant role in the hydrolytic activation of remdesivir in humans
Authors: Zhang, Feng and Li, Hong-Xin and Zhang, Tian-Tian and Xiong, Yuan and Wang, Hao-Nan and Lu, Zhen-Hui and Xiong, Lei and He, Yu-Qi and Ge, Guang-Bo
Journal: Chemico-biological interactions (2021): 109744
Authors: Zhang, Feng and Li, Hong-Xin and Zhang, Tian-Tian and Xiong, Yuan and Wang, Hao-Nan and Lu, Zhen-Hui and Xiong, Lei and He, Yu-Qi and Ge, Guang-Bo
Journal: Chemico-biological interactions (2021): 109744
Inhibition of drug-metabolizing enzymes by Jingyin granules: implications of herb--drug interactions in antiviral therapy
Authors: Zhang, Feng and Liu, Wei and Huang, Jian and Chen, Qi-long and Wang, Dan-dan and Zou, Li-wei and Zhao, Yong-fang and Zhang, Wei-dong and Xu, Jian-guang and Chen, Hong-zhuan and others,
Journal: Acta Pharmacologica Sinica (2021): 1--10
Authors: Zhang, Feng and Liu, Wei and Huang, Jian and Chen, Qi-long and Wang, Dan-dan and Zou, Li-wei and Zhao, Yong-fang and Zhang, Wei-dong and Xu, Jian-guang and Chen, Hong-zhuan and others,
Journal: Acta Pharmacologica Sinica (2021): 1--10
Inhibition of drug-metabolizing enzymes by Qingfei Paidu decoction: Implication of herb-drug interactions in COVID-19 pharmacotherapy
Authors: Zhang, Feng and Huang, Jian and Liu, Wei and Wang, Chao-Ran and Liu, Yan-Fang and Tu, Dong-Zhu and Liang, Xin-Miao and Yang, Ling and Zhang, Wei-Dong and Chen, Hong-Zhuan and others,
Journal: Food and Chemical Toxicology (2021): 111998
Authors: Zhang, Feng and Huang, Jian and Liu, Wei and Wang, Chao-Ran and Liu, Yan-Fang and Tu, Dong-Zhu and Liang, Xin-Miao and Yang, Ling and Zhang, Wei-Dong and Chen, Hong-Zhuan and others,
Journal: Food and Chemical Toxicology (2021): 111998
Carboxylesterase Activities and Protein Expression in Rabbit and Pig Ocular Tissues
Authors: Hammid, Anam and Fallon, John K and Lassila, Toni and Salluce, Giulia and Smith, Philip C and Tolonen, Ari and Sauer, Achim and Urtti, Arto and Honkakoski, Paavo
Journal: Molecular pharmaceutics (2021): 1305--1316
Authors: Hammid, Anam and Fallon, John K and Lassila, Toni and Salluce, Giulia and Smith, Philip C and Tolonen, Ari and Sauer, Achim and Urtti, Arto and Honkakoski, Paavo
Journal: Molecular pharmaceutics (2021): 1305--1316
Rapalogues as hCES2A Inhibitors: In Vitro and In Silico Investigations
Authors: Shi, Cheng-Cheng and Song, Yun-Qing and He, Rong-Jing and Guan, Xiao-Qing and Song, Li-Lin and Chen, Shi-Tong and Sun, Meng-Ru and Ge, Guang-Bo and Zhang, Li-Rong
Journal: European Journal of Drug Metabolism and Pharmacokinetics (2020): 1--11
Authors: Shi, Cheng-Cheng and Song, Yun-Qing and He, Rong-Jing and Guan, Xiao-Qing and Song, Li-Lin and Chen, Shi-Tong and Sun, Meng-Ru and Ge, Guang-Bo and Zhang, Li-Rong
Journal: European Journal of Drug Metabolism and Pharmacokinetics (2020): 1--11
C3-Luc Cells Are an Excellent Model for Evaluation of Cellular Immunity following HPV16L1 Vaccination
Authors: Li, Li-Li and Wang, He-Rong and Zhou, Zhi-Yi and Luo, Jing and Wang, Xiao-Li and Xiao, Xiang-Qian and Zhou, Yu-Bai and Zeng, Yi
Journal: PloS one (2016): e0149748
Authors: Li, Li-Li and Wang, He-Rong and Zhou, Zhi-Yi and Luo, Jing and Wang, Xiao-Li and Xiao, Xiang-Qian and Zhou, Yu-Bai and Zeng, Yi
Journal: PloS one (2016): e0149748
References
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Cloning and characterization of the homologous genes of firefly luciferase in the mealworm beetle, Tenebrio molitor
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Journal: Insect Mol Biol (2006): 293
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Effect of different salts and detergents on luciferin-luciferase luminescence of the enchytraeid Fridericia heliota
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Two different domains of the luciferase gene in the heterotrophic dinoflagellate Noctiluca scintillans occur as two separate genes in photosynthetic species
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Noninvasive bioluminescence imaging of luciferase expressing intracranial U87 xenografts: correlation with magnetic resonance imaging determined tumor volume and longitudinal use in assessing tumor growth and antiangiogenic treatment effect
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Bovine serum albumin displays luciferase-like activity in presence of luciferyl adenylate: insights on the origin of protoluciferase activity and bioluminescence colours
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Application notes
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
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Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severity
β-adrenoceptors are upregulated in human melanoma and their activation releases pro-tumorigenic cytokines and metalloproteases in melanoma cell lines
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
An Increase in Plasma Homovanillic Acid with Cocoa Extract Consumption Is Associated with the Alleviation of Depressive Symptoms in Overweight or Obese Adults
Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severity
β-adrenoceptors are upregulated in human melanoma and their activation releases pro-tumorigenic cytokines and metalloproteases in melanoma cell lines