DAPI Dilactate

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Physical properties

Molecular weight	457.48
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	27000
Excitation (nm)	359
Emission (nm)	457

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Overview SDS Protocol

CAS

28718-91-4

Molecular weight

457.48

Extinction coefficient (cm ^-1 M ^-1)

27000

Excitation (nm)

359

Emission (nm)

457

DAPI is a fluorescent stain that binds strongly to DNA. It is used extensively in fluorescence microscopy. Since DAPI passes through an intact cell membrane, it can be used to stain live cells besides fixed cells. For fluorescence microscopy, DAPI is excited with ultraviolet light. When bound to double-stranded DNA its absorption maximum is at 358 nm and its emission maximum is at 461 nm. One drawback of DAPI is that its emission is fairly broad. DAPI also binds to RNA although it is not as strongly fluorescent as it binds to DNA. Its emission shifts to around 500 nm when bound to RNA. DAPI's blue emission is convenient for multiplexing assays since there is very little fluorescence overlap between DAPI and green-fluorescent molecules like fluorescein and green fluorescent protein (GFP), or red-fluorescent stains like Texas Red. Besides labeling cell nuclei, DAPI is also used for the detection of mycoplasma or virus DNA in cell cultures. DAPI dilactate has improved water solubility compared to DAPI HCl salt.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of DAPI Dilactate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	218.589 µL	1.093 mL	2.186 mL	10.929 mL	21.859 mL
5 mM	43.718 µL	218.589 µL	437.178 µL	2.186 mL	4.372 mL
10 mM	21.859 µL	109.294 µL	218.589 µL	1.093 mL	2.186 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	27000
Excitation (nm)	359
Emission (nm)	457

Images

Figure 1. Chemical structure for DAPI Dilactate

References

View all 48 references: Citation Explorer

Differential pulse voltammetric studies of ethidium bromide binding to DNA
Authors: Minasyan SH, Tavadyan LA, Antonyan AP, Davtyan HG, Parsadanyan MA, Vardevanyan PO.
Journal: Bioelectrochemistry (2006): 48

Possible errors in the interpretation of ethidium bromide and PicoGreen DNA staining results from ethidium monoazide-treated DNA
Authors: Hein I, Flekna G, Wagner M, Nocker A, Camper AK.
Journal: Appl Environ Microbiol (2006): 6860

Ethidium bromide inhibits rat brain acetylcholinesterase activity in vitro
Authors: Mazzanti CM, Spanevello RM, Obregon A, Pereira LB, Streher CA, Ahmed M, Mazzanti A, Graca DL, Morsch VM, Schetinger MR.
Journal: Chem Biol Interact (2006): 121

Delayed Schwann cell and oligodendrocyte remyelination after ethidium bromide injection in the brainstem of Wistar rats submitted to streptozotocin diabetogenic treatment
Authors: Bondan EF, Lallo MA, Trigueiro AH, Ribeiro CP, Sinhorini IL, Graca DL.
Journal: Braz J Med Biol Res (2006): 637

A low dose of ethidium bromide leads to an increase of total mitochondrial DNA while higher concentrations induce the mtDNA 4997 deletion in a human neuronal cell line
Authors: von Wurmb-Schwark N, Cavelier L, Cortopassi GA.
Journal: Mutat Res (2006): 57

Determination of the DNA-binding characteristics of ethidium bromide, proflavine, and cisplatin by flow injection analysis: usefulness in studies on antitumor drugs
Authors: Alonso A, Almendral MJ, Curto Y, Criado JJ, Rodriguez E, Manzano JL.
Journal: Anal Biochem (2006): 157

Ethidium bromide is good not only for staining of nucleic acids but also for staining of proteins after polyacrylamide gel soaking in trichloroacetic acid solution
Authors: Dvortsov IA, Lunina NA, Chekanovskaya LA, Shedova EN, Gening LV, Velikodvorskaya GA.
Journal: Anal Biochem (2006): 293

Increase in mitochondrial DNA quantity and impairment of oxidative phosphorylation in bovine fibroblast cells treated with ethidium bromide for 15 passages in culture
Authors: Chiaratti MR, Meirelles FV.
Journal: Genet Mol Res (2006): 55

Apyrase and 5'-nucleotidase activities in synaptosomes from the cerebral cortex of rats experimentally demyelinated with ethidium bromide and treated with interferon-beta
Authors: Spanevello RM, Mazzanti CM, Kaizer R, Zanin R, Cargnelutti D, Hannel L, Correa M, Mazzanti A, Festugatto R, Graca D, Schetinger MR, Morsch VM.
Journal: Neurochem Res (2006): 455

The Complex of Ethidium Bromide with Genomic DNA: Structure Analysis by Polarized Raman Spectroscopy
Authors: Tsuboi M, Benevides JM, Thomas Jr GJ.
Journal: Biophys J. (2006)