Fluorescein-12-dGTP *1 mM in Tris Buffer (pH 7.5)*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1030.72 |
Solvent | Water |
Spectral properties
Absorbance (nm) | 487 |
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 800001 |
Excitation (nm) | 498 |
Emission (nm) | 517 |
Quantum yield | 0.79001, 0.952 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
Molecular weight 1030.72 | Absorbance (nm) 487 | Correction Factor (260 nm) 0.32 | Correction Factor (280 nm) 0.35 | Extinction coefficient (cm -1 M -1) 800001 | Excitation (nm) 498 | Emission (nm) 517 | Quantum yield 0.79001, 0.952 |
Fluorescein-12-dGTP might be incorporated into a DNA sequence as its natural counterpart dGTP to incorporate a fluorescein tag in a DNA molecule. It can be used for the direct enzymatic labeling of DNA/cDNA, e.g. by nick translation, random priming, polymerase chain reaction (PCR), and 3'-end labeling. The resulting fluorescein-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. The linker between fluorescein tag and guanosine has been optimized for the optimal substrate incorporation and labeling efficiency. The labeled products may be detected directly via fluorescence or indirectly by combining with anti-fluorescein HRP or AP conjugates.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Fluorescein-12-dGTP *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 97.02 µL | 485.098 µL | 970.196 µL | 4.851 mL | 9.702 mL |
5 mM | 19.404 µL | 97.02 µL | 194.039 µL | 970.196 µL | 1.94 mL |
10 mM | 9.702 µL | 48.51 µL | 97.02 µL | 485.098 µL | 970.196 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
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Spectral properties
Absorbance (nm) | 487 |
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 800001 |
Excitation (nm) | 498 |
Emission (nm) | 517 |
Quantum yield | 0.79001, 0.952 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
Fluorescein-12-dUTP *1 mM in TE Buffer (pH 7.5)* *CAS 214154-36-6* | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
Fluorescein-12-dUTP *1 mM in Tris Buffer (pH 7.5)* | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
Fluorescein-12-dCTP *1 mM in Tris Buffer (pH 7.5)* | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
Fluorescein-12-dATP *1 mM in Tris Buffer (pH 7.5)* | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
Application notes
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