NED Dye qPCR Calibration Solution *10,000X*
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Solvent | Water |
Absorbance (nm) | 544 |
Extinction coefficient (cm -1 M -1) | 740001 |
Excitation (nm) | 545 |
Emission (nm) | 567 |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | SDSProtocol |
Absorbance (nm) 544 | Extinction coefficient (cm -1 M -1) 740001 | Excitation (nm) 545 | Emission (nm) 567 |
Platform
qPCR
Recommended plate | PCR Microplate |
Instrument specification(s) | NED Filter |
Example protocol
SAMPLE EXPERIMENTAL PROTOCOL
This generic protocol is suitable for the ABI7500 FAST qPCR instrument. For complete instructions on use, refer to the maintenance guide for your instrument.
Prepare a NED Dye qPCR Calibration working solution by mixing 1 µL of the 10,000X NED Dye qPCR Calibration Solution with 10 mL of nuclease-free water or qPCR buffer.
Note: The final dye concentration must be optimized based on the calibration requirements for your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.
Add 20 µL of the NED Dye qPCR Calibration working solution to all wells of a reaction plate.
Note: The volume may vary depending on your instrument. Refer to the manufacturer’s maintenance guide for detailed instructions.
Seal the plate with an optical adhesive cover.
Centrifuge the plate for 2 minutes at < 1500 rpm.
Check that the liquid in each well of the plate is at the bottom of the well. If not, centrifuge the plate again at a slightly higher rpm and for a longer period of time.
For detailed calibration instructions, refer to the maintenance guide for your instrument.
Spectrum
Spectral properties
Absorbance (nm) | 544 |
Extinction coefficient (cm -1 M -1) | 740001 |
Excitation (nm) | 545 |
Emission (nm) | 567 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
NED Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 545 | 567 | 740001 |
VIC Dye qPCR Calibration Solution *10,000X* | 526 | 543 | - |
FAM Dye qPCR Calibration Solution *10,000X* | 493 | 517 | 83000 |
Cy5 Dye qPCR Calibration Solution *10,000X* | 651 | 670 | 2500001 |
ROX Dye qPCR Calibration Solution *10,000X* | 578 | 595 | - |
SYBR Dye qPCR Calibration Solution *10,000X* | 498 | 522 | - |
TAMRA Dye qPCR Calibration Solution *10,000X* | 552 | 578 | 90000 |
ABY Dye qPCR Calibration Solution *10,000X* | 559 | 570 | 1200001 |
JUN Dye qPCR Calibration Solution *10,000X* | 610 | 628 | 1100001 |
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References
Authors: Can, Ziya and Keskin, Büşra and Üzer, Ayşem and Apak, Reşat
Journal: Talanta (2022): 122993
Authors: Bilgrami, Afreen and Alam, Mohammad Khursheed and Qazi, Fazal Ur Rehman and Maqsood, Afsheen and Basha, Sakeenabi and Ahmed, Naseer and Syed, Kausar Ali and Mustafa, Mohammed and Shrivastava, Deepti and Nagarajappa, Anil Kumar and Srivastava, Kumar Chandan
Journal: Polymers (2022)
Authors: Mamatioğlu, Fatma and Üzer, Ayşem and Erçağ, Erol and Apak, Reşat
Journal: Talanta (2021): 122187
Authors: Lozano-Peral, Diego and Rubio, Leticia and Santos, Ignacio and Gaitán, María Jesús and Viguera, Enrique and Martín-de-Las-Heras, Stella
Journal: Scientific reports (2021): 12118
Authors: Zhou, Xiaohong and Memon, Abdul Ghaffar and Sun, Weiming and Fang, Fang and Guo, Jinsong
Journal: Biosensors (2020)
Authors: Khan, Tabinda Nawaz and Abidi, Syed Yawar Ali
Journal: Journal of the College of Physicians and Surgeons--Pakistan : JCPSP (2018): 9-12
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82
Authors: Khan, Tabinda Nawaz and Ali Abidi, Syed Yawar and Nawaz Khan, Khush Bakht and Ahmed, Shahbaz and Rehman Qazi, Fazal Ur and Saeed, Noureen
Journal: Journal of the College of Physicians and Surgeons--Pakistan : JCPSP (2015): 781-4
Authors: Üzer, Ayşem and Can, Ziya and Akın, Ilknur and Erçağ, Erol and Apak, Reşat
Journal: Analytical chemistry (2014): 351-6
Authors: Chung, Seung Won and Park, Il-Ho and Hong, Sung-Moon and Cho, Jung-Sun and Moon, Jun-Hyeok and Kim, Tae Hoon and Lee, Heung-Man
Journal: Clinical and experimental otorhinolaryngology (2014): 295-301
Application notes
FAQ
Are mRNAs found in prokaryotes differ from those of eukaryotes?
Are spliceosomes associated with any diseases?
Are there any alternatives for ethidium bromide in agarose gels?
Are there any alternatives to Cy5?