NED Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | N/A |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 544 |
Extinction coefficient (cm -1 M -1) | 740001 |
Excitation (nm) | 545 |
Emission (nm) | 567 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Real-Time PCR (qPCR)
Molecular weight N/A | Absorbance (nm) 544 | Extinction coefficient (cm -1 M -1) 740001 | Excitation (nm) 545 | Emission (nm) 567 |
NED Dye qPCR Calibration Plate can be used to maintain your 7500 Real-Time PCR system with Fast 96-well block. For most of qPCR instruments, the necessary calibrations should be run at least every six months. This calibration plate is ready to use without any additional preparation steps required. The qPCR calibration plate might significantly improve qPCR results with multiplexing by more accurately representing fluorescent spectra used in your real-time experiments. Please refer to your instrument's guide for the detailed calibration operation.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Absorbance (nm) | 544 |
Extinction coefficient (cm -1 M -1) | 740001 |
Excitation (nm) | 545 |
Emission (nm) | 567 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
TAMRA Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 552 | 578 | 90000 |
VIC Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 526 | 543 | - |
ROX Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 578 | 604 | 82000 |
FAM Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 493 | 517 | 83000 |
SYBR Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 498 | 522 | - |
JOE Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 520 | 545 | 750001 |
NED Dye qPCR Calibration Solution *10,000X* | 545 | 567 | 740001 |
Cy5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 651 | 670 | 2500001 |
ABY Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 559 | 570 | 1200001 |
Show More (2) |
References
View all 12 references: Citation Explorer
DNA degradation in human teeth exposed to thermal stress.
Authors: Lozano-Peral, Diego and Rubio, Leticia and Santos, Ignacio and Gaitán, María Jesús and Viguera, Enrique and Martín-de-Las-Heras, Stella
Journal: Scientific reports (2021): 12118
Authors: Lozano-Peral, Diego and Rubio, Leticia and Santos, Ignacio and Gaitán, María Jesús and Viguera, Enrique and Martín-de-Las-Heras, Stella
Journal: Scientific reports (2021): 12118
Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay.
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82
Multicolor-based discrimination of 21 short tandem repeats and amelogenin using four fluorescent universal primers.
Authors: Asari, Masaru and Okuda, Katsuhiro and Hoshina, Chisato and Omura, Tomohiro and Tasaki, Yoshikazu and Shiono, Hiroshi and Matsubara, Kazuo and Shimizu, Keiko
Journal: Analytical biochemistry (2016): 16-22
Authors: Asari, Masaru and Okuda, Katsuhiro and Hoshina, Chisato and Omura, Tomohiro and Tasaki, Yoshikazu and Shiono, Hiroshi and Matsubara, Kazuo and Shimizu, Keiko
Journal: Analytical biochemistry (2016): 16-22
Multiplex-Ready Technology for mid-throughput genotyping of molecular markers.
Authors: Bonneau, Julien and Hayden, Matthew
Journal: Methods in molecular biology (Clifton, N.J.) (2014): 47-57
Authors: Bonneau, Julien and Hayden, Matthew
Journal: Methods in molecular biology (Clifton, N.J.) (2014): 47-57
An improved rapid quantitative detection and identification method for a wide range of fungi.
Authors: Soeta, Nobutoshi and Terashima, Masanori and Gotoh, Mitsukazu and Mori, Shuichi and Nishiyama, Kyoko and Ishioka, Ken and Kaneko, Hisatoshi and Suzutani, Tatsuo
Journal: Journal of medical microbiology (2009): 1037-1044
Authors: Soeta, Nobutoshi and Terashima, Masanori and Gotoh, Mitsukazu and Mori, Shuichi and Nishiyama, Kyoko and Ishioka, Ken and Kaneko, Hisatoshi and Suzutani, Tatsuo
Journal: Journal of medical microbiology (2009): 1037-1044
Internally controlled triplex quantitative PCR assay for human polyomaviruses JC and BK.
Authors: Dumonceaux, Timothy J and Mesa, Christine and Severini, Alberto
Journal: Journal of clinical microbiology (2008): 2829-36
Authors: Dumonceaux, Timothy J and Mesa, Christine and Severini, Alberto
Journal: Journal of clinical microbiology (2008): 2829-36
TaqMan reverse transcriptase-polymerase chain reaction coupled with capillary electrophoresis for quantification and identification of bcr-abl transcript type.
Authors: Luthra, Rajyalakshmi and Medeiros, L Jeffrey
Journal: Methods in molecular biology (Clifton, N.J.) (2006): 135-45
Authors: Luthra, Rajyalakshmi and Medeiros, L Jeffrey
Journal: Methods in molecular biology (Clifton, N.J.) (2006): 135-45
TaqMan RT-PCR assay coupled with capillary electrophoresis for quantification and identification of bcr-abl transcript type.
Authors: Luthra, Rajyalakshmi and Sanchez-Vega, Beatriz and Medeiros, L Jeffrey
Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (2004): 96-103
Authors: Luthra, Rajyalakshmi and Sanchez-Vega, Beatriz and Medeiros, L Jeffrey
Journal: Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc (2004): 96-103
Semiquantitative and qualitative assessment of B-lymphocyte V H repertoire by a fluorescent multiplex PCR.
Authors: Feuchtenberger, Martin and Tony, Hans-Peter and Rouzière, Anne-Sophie and Jacobi, Anette and Dörner, Thomas and Kneitz, Christian and Starostik, Petr
Journal: Journal of immunological methods (2003): 121-7
Authors: Feuchtenberger, Martin and Tony, Hans-Peter and Rouzière, Anne-Sophie and Jacobi, Anette and Dörner, Thomas and Kneitz, Christian and Starostik, Petr
Journal: Journal of immunological methods (2003): 121-7
Development of a 17-plex microsatellite polymerase chain reaction kit for genotyping horses.
Authors: Dimsoski, Pero
Journal: Croatian medical journal (2003): 332-5
Authors: Dimsoski, Pero
Journal: Croatian medical journal (2003): 332-5
Application notes
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are mRNAs found in prokaryotes differ from those of eukaryotes?
Are spliceosomes associated with any diseases?
Are there any alternatives for ethidium bromide in agarose gels?
Are there any alternatives to Cy5?
Are mRNAs found in prokaryotes differ from those of eukaryotes?
Are spliceosomes associated with any diseases?
Are there any alternatives for ethidium bromide in agarose gels?
Are there any alternatives to Cy5?