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Cy5 Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Spectral properties
| Correction Factor (260 nm) | 0.02 |
| Correction Factor (280 nm) | 0.03 |
| Correction Factor (482 nm) | 0.009 |
| Correction Factor (565 nm) | 0.09 |
| Extinction coefficient (cm -1 M -1) | 2500001 |
| Excitation (nm) | 651 |
| Emission (nm) | 670 |
| Quantum yield | 0.271, 0.42 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| Overview |  SDSProtocol |
See also: Real-Time PCR (qPCR)
Correction Factor (260 nm) 0.02 | Correction Factor (280 nm) 0.03 | Correction Factor (482 nm) 0.009 | Correction Factor (565 nm) 0.09 | Extinction coefficient (cm -1 M -1) 2500001 | Excitation (nm) 651 | Emission (nm) 670 | Quantum yield 0.271, 0.42 |
A Cy5 dye qPCR calibration plate is a type of plate used in quantitative PCR (polymerase chain reaction) experiments. It is designed to calibrate the real-time PCR instrument, which measures the fluorescence signal emitted by the Cy5 dye during the PCR reaction. The Cy5 dye qPCR calibration plate contains a set of pre-determined concentrations of a DNA template that serves as a reference for the quantification of unknown samples. The plate is loaded into the PCR instrument along with the unknown samples, and the instrument measures the fluorescence signal from each well during the PCR amplification process. By comparing the fluorescence signal of the unknown samples to the known concentrations in the calibration plate, researchers can determine the amount of DNA present in the unknown samples. This information can be used to quantify gene expression levels, detect the presence of pathogens, or perform other types of molecular analysis.
Spectrum
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Spectral properties
| Correction Factor (260 nm) | 0.02 |
| Correction Factor (280 nm) | 0.03 |
| Correction Factor (482 nm) | 0.009 |
| Correction Factor (565 nm) | 0.09 |
| Extinction coefficient (cm -1 M -1) | 2500001 |
| Excitation (nm) | 651 |
| Emission (nm) | 670 |
| Quantum yield | 0.271, 0.42 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| Cy5 Dye qPCR Calibration Solution *10,000X* | 651 | 670 | 2500001 |
| TAMRA Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 552 | 578 | 90000 |
| VIC Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 526 | 543 | - |
| ROX Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 578 | 604 | 82000 |
| FAM Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 493 | 517 | 83000 |
| SYBR Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 498 | 522 | - |
| NED Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 545 | 567 | 740001 |
| JOE Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 520 | 545 | 750001 |
| ABY Dye qPCR Calibration Plate *Optimized for ABI7500 Fast 96-Well* | 559 | 570 | 1200001 |
Show More (2) | |||
References
View all 50 references: Citation Explorer
Evaluating Novel Quantification Methods for Infectious Baculoviruses.
Authors: Lothert, Keven and Bagrin, Elena and Wolff, Michael W
Journal: Viruses (2023)
Authors: Lothert, Keven and Bagrin, Elena and Wolff, Michael W
Journal: Viruses (2023)
Investigation of the shared molecular mechanisms and hub genes between myocardial infarction and depression.
Authors: Wang, Mengxi and Cheng, Liying and Gao, Ziwei and Li, Jianghong and Ding, Yuhan and Shi, Ruijie and Xiang, Qian and Chen, Xiaohu
Journal: Frontiers in cardiovascular medicine (2023): 1203168
Authors: Wang, Mengxi and Cheng, Liying and Gao, Ziwei and Li, Jianghong and Ding, Yuhan and Shi, Ruijie and Xiang, Qian and Chen, Xiaohu
Journal: Frontiers in cardiovascular medicine (2023): 1203168
Identification and Validation of a Novel Glycolysis-Related Gene Signature for Predicting the Prognosis and Therapeutic Response in Triple-Negative Breast Cancer.
Authors: Zheng, Jian and Zhang, Yi-Fan and Han, Guo-Hui and Fan, Meng-Ying and Du, Ming-Hui and Zhang, Guo-Chen and Zhang, Bo and Qiao, Jun and Zhang, Sheng-Xiao and Cao, Ji-Min
Journal: Advances in therapy (2023): 310-330
Authors: Zheng, Jian and Zhang, Yi-Fan and Han, Guo-Hui and Fan, Meng-Ying and Du, Ming-Hui and Zhang, Guo-Chen and Zhang, Bo and Qiao, Jun and Zhang, Sheng-Xiao and Cao, Ji-Min
Journal: Advances in therapy (2023): 310-330
Comprehensive analysis of oxidative stress-related lncRNA signatures in glioma reveals the discrepancy of prognostic and immune infiltration.
Authors: Shi, Zhenyi and Wu, Yingying and Zhuo, Qingchan and Zuo, Yufang and Lin, Jiong and Shi, Huadi and Zhou, Hechao and Xu, Zumin
Journal: Scientific reports (2023): 7731
Authors: Shi, Zhenyi and Wu, Yingying and Zhuo, Qingchan and Zuo, Yufang and Lin, Jiong and Shi, Huadi and Zhou, Hechao and Xu, Zumin
Journal: Scientific reports (2023): 7731
Regional and temporal differences in the relation between SARS-CoV-2 biomarkers in wastewater and estimated infection prevalence - Insights from long-term surveillance.
Authors: Helm, Björn and Geissler, Michael and Mayer, Robin and Schubert, Sara and Oertel, Reinhard and Dumke, Roger and Dalpke, Alexander and El-Armouche, Ali and Renner, Bertold and Krebs, Peter
Journal: The Science of the total environment (2023): 159358
Authors: Helm, Björn and Geissler, Michael and Mayer, Robin and Schubert, Sara and Oertel, Reinhard and Dumke, Roger and Dalpke, Alexander and El-Armouche, Ali and Renner, Bertold and Krebs, Peter
Journal: The Science of the total environment (2023): 159358
C1QC, VSIG4, and CFD as Potential Peripheral Blood Biomarkers in Atrial Fibrillation-Related Cardioembolic Stroke.
Authors: Ding, Qian and Xing, Juan and Bai, Fanghui and Shao, Wei and Hou, Kaiqi and Zhang, Shoudu and Hu, Yuanzheng and Zhang, Baochao and Zhao, Hui and Xu, Qian
Journal: Oxidative medicine and cellular longevity (2023): 5199810
Authors: Ding, Qian and Xing, Juan and Bai, Fanghui and Shao, Wei and Hou, Kaiqi and Zhang, Shoudu and Hu, Yuanzheng and Zhang, Baochao and Zhao, Hui and Xu, Qian
Journal: Oxidative medicine and cellular longevity (2023): 5199810
Clinical value of anoikis-related genes and molecular subtypes identification in bladder urothelial carcinoma and in vitro validation.
Authors: Dong, Ying and Xu, Chaojie and Su, Ganglin and Li, Yanfeng and Yan, Bing and Liu, Yuhan and Yin, Tao and Mou, Shuanzhu and Mei, Hongbing
Journal: Frontiers in immunology (2023): 1122570
Authors: Dong, Ying and Xu, Chaojie and Su, Ganglin and Li, Yanfeng and Yan, Bing and Liu, Yuhan and Yin, Tao and Mou, Shuanzhu and Mei, Hongbing
Journal: Frontiers in immunology (2023): 1122570
Novel Cell Quantification Method Using a Single Surrogate Calibration Curve Across Various Biological Samples.
Authors: Nakayama, Miyu and Yamamoto, Syunsuke and Hirabayashi, Hideki
Journal: The AAPS journal (2023): 26
Authors: Nakayama, Miyu and Yamamoto, Syunsuke and Hirabayashi, Hideki
Journal: The AAPS journal (2023): 26
Detecting the Neuraminidase R294K Mutation in Avian Influenza A (H7N9) Virus Using Reverse Transcription Droplet Digital PCR Method.
Authors: Lou, Xiuyu and Yan, Hao and Su, Lingxuan and Sun, Yi and Wang, Xinyin and Gong, Liming and Chen, Yin and Li, Zhen and Fang, Zhongbiao and Mao, Haiyan and Chen, Keda and Zhang, Yanjun
Journal: Viruses (2023)
Authors: Lou, Xiuyu and Yan, Hao and Su, Lingxuan and Sun, Yi and Wang, Xinyin and Gong, Liming and Chen, Yin and Li, Zhen and Fang, Zhongbiao and Mao, Haiyan and Chen, Keda and Zhang, Yanjun
Journal: Viruses (2023)
A quadruple fluorescence quantitative PCR method for the identification of wild strains of african swine fever and gene-deficient strains.
Authors: Zuo, Xuezhi and Peng, Guorui and Xia, Yingju and Xu, Lu and Zhao, Qizu and Zhu, Yuanyuan and Wang, Cheng and Liu, Yebing and Zhao, Junjie and Wang, Haidong and Zou, Xingqi
Journal: Virology journal (2023): 150
Authors: Zuo, Xuezhi and Peng, Guorui and Xia, Yingju and Xu, Lu and Zhao, Qizu and Zhu, Yuanyuan and Wang, Cheng and Liu, Yebing and Zhao, Junjie and Wang, Haidong and Zou, Xingqi
Journal: Virology journal (2023): 150
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?