Protonex™ Lyso-NIR 770
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 736.76 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 748 |
Emission (nm) | 769 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Overview | SDSProtocol |
Molecular weight 736.76 | Excitation (nm) 748 | Emission (nm) 769 |
Protonex™ Lyso-NIR 770 is a fluorescent derivative prepared from our Protonex™ NIR 770 NHS ester. It is cell-permeable and selectively localized in acidic organelles such as lysosomes. It is the only cell-permeable acidotropic pH dye with NIR fluorescence for monitoring pH changes in acidic organelles. Its fluorescence can be readily captured with the common Cy7 filter set that is equipped with most of the fluorescence instruments. Due to its significantly red-shifted excitation and emission wavelengths, it has much lower background than the existing pH sensors (such as Lysosensors). It might be used in vivo for monitoring pH changes in animals due to the significantly improved skin-penetration capability of NIR excitation.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Protonex™ Lyso-NIR 770 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 135.729 µL | 678.647 µL | 1.357 mL | 6.786 mL | 13.573 mL |
5 mM | 27.146 µL | 135.729 µL | 271.459 µL | 1.357 mL | 2.715 mL |
10 mM | 13.573 µL | 67.865 µL | 135.729 µL | 678.647 µL | 1.357 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
Figure 1. Image of Hela cells stained with Protonex™ Lyso-NIR 770. Hela cells immersed in a cell culture medium were loaded with 5 µM of Protonex™ Lyso-NIR 770 in HH buffer per well and incubated at 37⁰C for 30 min. The cells were then washed 3 times with HH buffer, and 100 µL of fresh cell culture media was added to each well. The images were captured in the Cy7 channel. Additionally, the cells were co-stained with LysoBrite™ Red (Cat# 22645), with images acquired in the TRITC channel but presented with a pseudo-Green Color. The overlay images reveal that Protonex™ Lyso-NIR 770 effectively stains the lysosome of HeLa cells.
References
View all 50 references: Citation Explorer
Rational design of a ratiometric fluorescent probe for imaging lysosomal nitroreductase activity.
Authors: Bai, Wenjun and Li, Yixuan and Zhao, Li and Li, Ruxin and Geng, Jiahou and Lu, Yang and Zhao, Yufen and Wang, Jinhui
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2023): 123032
Authors: Bai, Wenjun and Li, Yixuan and Zhao, Li and Li, Ruxin and Geng, Jiahou and Lu, Yang and Zhao, Yufen and Wang, Jinhui
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2023): 123032
A potent fluorescent transmembrane HCl transporter perturbs cellular pH and promotes cancer cell death.
Authors: Fares, Mohamed and Wu, Xin and McNaughton, Daniel A and Gilchrist, Alexander M and Lewis, William and Keller, Paul A and Arias-Betancur, Alain and Fontova, Pere and Pérez-Tomás, Ricardo and Gale, Philip A
Journal: Organic & biomolecular chemistry (2023): 2509-2515
Authors: Fares, Mohamed and Wu, Xin and McNaughton, Daniel A and Gilchrist, Alexander M and Lewis, William and Keller, Paul A and Arias-Betancur, Alain and Fontova, Pere and Pérez-Tomás, Ricardo and Gale, Philip A
Journal: Organic & biomolecular chemistry (2023): 2509-2515
Analysis of Radachlorin localization in living cells by fluorescence lifetime imaging microscopy.
Authors: Belashov, A V and Zhikhoreva, A A and Salova, A V and Belyaeva, T N and Litvinov, I K and Kornilova, E S and Semenova, I V and Vasyutinskii, O S
Journal: Journal of photochemistry and photobiology. B, Biology (2023): 112699
Authors: Belashov, A V and Zhikhoreva, A A and Salova, A V and Belyaeva, T N and Litvinov, I K and Kornilova, E S and Semenova, I V and Vasyutinskii, O S
Journal: Journal of photochemistry and photobiology. B, Biology (2023): 112699
In vivo Assessment of Lysosomal Stress in the Drosophila Brain Using Confocal Fluorescence Microscopy.
Authors: Martelli, Felipe
Journal: Bio-protocol (2023): e4599
Authors: Martelli, Felipe
Journal: Bio-protocol (2023): e4599
A water-soluble fluorescent pH probe and its application for monitoring lysosomal pH changes in living cells.
Authors: Zhang, Lei and Guo, Jun and You, Qihua and Xu, Yuqing
Journal: Analytical methods : advancing methods and applications (2023): 3057-3063
Authors: Zhang, Lei and Guo, Jun and You, Qihua and Xu, Yuqing
Journal: Analytical methods : advancing methods and applications (2023): 3057-3063
A novel AIE fluorescence probe featuring with high quantum yield for high-fidelity lysosomal targeting and tracking.
Authors: Zhang, Pan and Xue, Ke and Dai, Yanpeng and Zhao, Xinxin and Zhang, Dongdong and Wei, Pengfei and Qi, Zhengjian
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2023): 122657
Authors: Zhang, Pan and Xue, Ke and Dai, Yanpeng and Zhao, Xinxin and Zhang, Dongdong and Wei, Pengfei and Qi, Zhengjian
Journal: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2023): 122657
A Review of Reagents for Fluorescence Microscopy of Cellular Compartments and Structures, Part I: BacMam Labeling and Reagents for Vesicular Structures.
Authors: Dolman, Nick J and Kilgore, Jason A
Journal: Current protocols (2023): e751
Authors: Dolman, Nick J and Kilgore, Jason A
Journal: Current protocols (2023): e751
A series of meso amide BODIPY based lysosome-targeting fluorescent probe with high photostability and sensitivity.
Authors: Ruan, Li and Bai, Junping and Ji, Xin and Zhao, Weili and Dong, Xiaochun
Journal: Analytica chimica acta (2022): 339771
Authors: Ruan, Li and Bai, Junping and Ji, Xin and Zhao, Weili and Dong, Xiaochun
Journal: Analytica chimica acta (2022): 339771
Visualizing Mitophagy with Fluorescent Dyes for Mitochondria and Lysosome.
Authors: Liu, Bilin and Li, Anqi and Qin, Yuan and Chen, Lei and Gao, Meng and Gong, Guohua
Journal: Journal of visualized experiments : JoVE (2022)
Authors: Liu, Bilin and Li, Anqi and Qin, Yuan and Chen, Lei and Gao, Meng and Gong, Guohua
Journal: Journal of visualized experiments : JoVE (2022)
Cell-imaging studies of highly substituted oxazole derivatives as organelle targeting fluorophores (OTFPs).
Authors: Adhikary, Saswati and Mukherjee, Kaustuv and Banerji, Biswadip
Journal: Scientific reports (2022): 16555
Authors: Adhikary, Saswati and Mukherjee, Kaustuv and Banerji, Biswadip
Journal: Scientific reports (2022): 16555