Protonex™ Red 780 acid
Ordering information
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Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 857.18 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 748 |
Emission (nm) | 769 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
See also: Intracellular pH
Molecular weight 857.18 | Excitation (nm) 748 | Emission (nm) 769 |
Protonex™ Red 780 works by changing its fluorescence intensity depending on the pH of the environment. Protonex™ Red 780 is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH. When Protonex™ Red 780 is bound to a receptor or an antibody on the cell surface, it is essentially non-fluorescent because the extracellular pH is neutral. However, when the receptor or antibody is internalized into the cell in response to a stimulus, it enters the endosomal pathway, where the pH is acidic. This causes Protonex™ Red 780 to become highly fluorescent and emit near-infrared light when excited by a red laser. By measuring the fluorescence intensity of Protonex™ Red 780, one can monitor the activation and trafficking of receptors or antibodies in live cells. Protonex™ Red 780 is especially useful in studying the activation and trafficking of G protein-coupled receptors (GPCRs), one of the most popular therapeutic drug targets. Protonex™ Red 780 can be used to label any receptor or epitope tag antibody and monitor its movement from the cell surface into acidic endosomes upon agonist stimulation. Protonex™ Red 780 might also be used to measure high-potency agonist and antagonist responses of different GPCRs in live cells.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Protonex™ Red 780 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 116.662 µL | 583.308 µL | 1.167 mL | 5.833 mL | 11.666 mL |
5 mM | 23.332 µL | 116.662 µL | 233.323 µL | 1.167 mL | 2.333 mL |
10 mM | 11.666 µL | 58.331 µL | 116.662 µL | 583.308 µL | 1.167 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Product Family
Name | Excitation (nm) | Emission (nm) |
Protonex™ Red 670 acid | 643 | 660 |
Protonex™ Red 780 AM *Cell-Permeable* | 748 | 769 |
mFluor™ Red 780 acid | 629 | 767 |
References
View all 50 references: Citation Explorer
Generalization of the Ratiometric Method to Extend pH Range Measurements of the BCECF Probe.
Authors: Tafech, Alaa and Beaujean, Céline and Usson, Yves and Stéphanou, Angélique
Journal: Biomolecules (2023)
Authors: Tafech, Alaa and Beaujean, Céline and Usson, Yves and Stéphanou, Angélique
Journal: Biomolecules (2023)
mOrange2, a Genetically Encoded, pH Sensitive Fluorescent Protein, is an Alternative to BCECF-AM to Measure Intracellular pH to Determine NHE3 and DRA Activity.
Authors: Sarker, Rafiquel and Tse, Chung Ming and Lin, Ruxian and McNamara, George and Singh, Varsha and Donowitz, Mark
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2022): 39-49
Authors: Sarker, Rafiquel and Tse, Chung Ming and Lin, Ruxian and McNamara, George and Singh, Varsha and Donowitz, Mark
Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology (2022): 39-49
Energy Metabolism and Intracellular pH Alteration in Neural Spheroids Carrying Down Syndrome.
Authors: Kashirina, Alena and Gavrina, Alena and Kryukov, Emil and Elagin, Vadim and Kolesova, Yuliya and Artyuhov, Alexander and Momotyuk, Ekaterina and Abdyyev, Vepa and Meshcheryakova, Natalia and Zagaynova, Elena and Dashinimaev, Erdem and Kashina, Aleksandra
Journal: Biomedicines (2021)
Authors: Kashirina, Alena and Gavrina, Alena and Kryukov, Emil and Elagin, Vadim and Kolesova, Yuliya and Artyuhov, Alexander and Momotyuk, Ekaterina and Abdyyev, Vepa and Meshcheryakova, Natalia and Zagaynova, Elena and Dashinimaev, Erdem and Kashina, Aleksandra
Journal: Biomedicines (2021)
Plastidial transporters KEA1 and KEA2 at the inner envelope membrane adjust stromal pH in the dark.
Authors: Aranda Sicilia, María Nieves and Sánchez Romero, María Elena and Rodríguez Rosales, María Pilar and Venema, Kees
Journal: The New phytologist (2021): 2080-2090
Authors: Aranda Sicilia, María Nieves and Sánchez Romero, María Elena and Rodríguez Rosales, María Pilar and Venema, Kees
Journal: The New phytologist (2021): 2080-2090
Effects of Diluent pH on Enrichment and Performance of Dairy Goat X/Y Sperm.
Authors: He, Qifu and Wu, Shenghui and Huang, Ming and Wang, Ying and Zhang, Kang and Kang, Jian and Zhang, Yong and Quan, Fusheng
Journal: Frontiers in cell and developmental biology (2021): 747722
Authors: He, Qifu and Wu, Shenghui and Huang, Ming and Wang, Ying and Zhang, Kang and Kang, Jian and Zhang, Yong and Quan, Fusheng
Journal: Frontiers in cell and developmental biology (2021): 747722
Glomerular Mesangial Cell pH Homeostasis Mediates Mineralocorticoid Receptor-Induced Cell Proliferation.
Authors: Gekle, Michael and Mildenberger, Sigrid
Journal: Biomedicines (2021)
Authors: Gekle, Michael and Mildenberger, Sigrid
Journal: Biomedicines (2021)
Intracellular pH regulation in mantle epithelial cells of the Pacific oyster, Crassostrea gigas.
Authors: Ramesh, Kirti and Hu, Marian Y and Melzner, Frank and Bleich, Markus and Himmerkus, Nina
Journal: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology (2020): 691-700
Authors: Ramesh, Kirti and Hu, Marian Y and Melzner, Frank and Bleich, Markus and Himmerkus, Nina
Journal: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology (2020): 691-700
Functional effects of urotensin-II on intracellular pH regulators in human radial artery smooth muscle cells.
Authors: Loh, Shih-Hurng and Chang, Chung-Yi and Huang, Shu-Fu and Chao, Shih-Chi and Lin, Wei-Kuo and Huang, Eagle Yi-Kung and Tsai, Chien-Sung and Tsai, Yi-Ting
Journal: Peptides (2020): 170236
Authors: Loh, Shih-Hurng and Chang, Chung-Yi and Huang, Shu-Fu and Chao, Shih-Chi and Lin, Wei-Kuo and Huang, Eagle Yi-Kung and Tsai, Chien-Sung and Tsai, Yi-Ting
Journal: Peptides (2020): 170236
Altered Degranulation and pH of Neutrophil Phagosomes Impacts Antimicrobial Efficiency in Cystic Fibrosis.
Authors: Hayes, Elaine and Murphy, Mark P and Pohl, Kerstin and Browne, Niall and McQuillan, Karen and Saw, Le Er and Foley, Clare and Gargoum, Fatma and McElvaney, Oliver J and Hawkins, Padraig and Gunaratnam, Cedric and McElvaney, Noel G and Reeves, Emer P
Journal: Frontiers in immunology (2020): 600033
Authors: Hayes, Elaine and Murphy, Mark P and Pohl, Kerstin and Browne, Niall and McQuillan, Karen and Saw, Le Er and Foley, Clare and Gargoum, Fatma and McElvaney, Oliver J and Hawkins, Padraig and Gunaratnam, Cedric and McElvaney, Noel G and Reeves, Emer P
Journal: Frontiers in immunology (2020): 600033
Fluorescence Measurement and Calibration of Intracellular pH in Starfish Oocytes.
Authors: Hosoda, Enako and Chiba, Kazuyoshi
Journal: Bio-protocol (2020): e3778
Authors: Hosoda, Enako and Chiba, Kazuyoshi
Journal: Bio-protocol (2020): e3778
Application notes
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Cell Preparation Considerations and Troubleshooting
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A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Cell Preparation Considerations and Troubleshooting
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
FAQ
How to measure intracellular pH using flow cytometry?
I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?
What is an AM ester?
What are the disadvantages of Nanodrop light spectrophotometer?
What are the advantages of a Nanodrop light spectrophotometer?
I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?
What is an AM ester?
What are the disadvantages of Nanodrop light spectrophotometer?
What are the advantages of a Nanodrop light spectrophotometer?
AssayWise
Intracellular pH Measurement with Dual Excitation Fluorescence Sensor BCFL
RATIOWORKS pH 165, AM: A Dual Excitation/Emission Fluorescence Probe For Imaging Live Cells
Investigating Intercellular Channels: Focus on Gap Junctions
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RATIOWORKS pH 165, AM: A Dual Excitation/Emission Fluorescence Probe For Imaging Live Cells
Investigating Intercellular Channels: Focus on Gap Junctions
Nucleic Acid Detection, Quantification and Imaging
Cal-520® AM, a Development Overview