Protonex™ Red 670, NHS ester
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 827.02 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 643 |
Emission (nm) | 660 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | SDSProtocol |
See also: Intracellular pH
Molecular weight 827.02 | Excitation (nm) 643 | Emission (nm) 660 |
Protonex™ Red 670 NHS ester is an excellent building block for developing a target-specific conjugate that can be used for monitoring a pH-sensitive biological event. Protonex™ Red 670 dye works by changing its fluorescence intensity depending on the pH of the environment. Protonex™ Red 670 is minimally fluorescent at a basic pH and maximally fluorescent at an acidic pH. When Protonex™ Red 670 is bound to a receptor or an antibody on the cell surface, it is essentially non-fluorescent because the extracellular pH is neutral. However, when the receptor or antibody is internalized into the cell in response to a stimulus, it enters the endosomal pathway, where the pH is acidic. This causes Protonex™ Red 670 to become highly fluorescent and emit red light when excited by a red laser such as a 632 nm He-Ne or 647 nm red laser. By measuring the fluorescence intensity of Protonex™ Red 670, one can monitor the activation and trafficking of receptors or antibodies in live cells.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Protonex™ Red 670, NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 120.916 µL | 604.58 µL | 1.209 mL | 6.046 mL | 12.092 mL |
5 mM | 24.183 µL | 120.916 µL | 241.832 µL | 1.209 mL | 2.418 mL |
10 mM | 12.092 µL | 60.458 µL | 120.916 µL | 604.58 µL | 1.209 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Application notes
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Cell Preparation Considerations and Troubleshooting
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FAQ
How to measure intracellular pH using flow cytometry?
I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?
What is an AM ester?
What are the disadvantages of Nanodrop light spectrophotometer?
What are the advantages of a Nanodrop light spectrophotometer?
I am performing a phagocytosis assay of macrophages engulfing Protonex Red 600 labeled tumor cells. How do you recommend I label my cells?
What is an AM ester?
What are the disadvantages of Nanodrop light spectrophotometer?
What are the advantages of a Nanodrop light spectrophotometer?
AssayWise
Intracellular pH Measurement with Dual Excitation Fluorescence Sensor BCFL
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RATIOWORKS pH 165, AM: A Dual Excitation/Emission Fluorescence Probe For Imaging Live Cells
Investigating Intercellular Channels: Focus on Gap Junctions
Nucleic Acid Detection, Quantification and Imaging
Cal-520® AM, a Development Overview