trFluor™ Eu DBCO *europium complex*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1765.72 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.911 |
Correction Factor (280 nm) | 0.777 |
Extinction coefficient (cm -1 M -1) | 21000 |
Excitation (nm) | 298 |
Emission (nm) | 617 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Overview | SDSProtocol |
Molecular weight 1765.72 | Correction Factor (260 nm) 0.911 | Correction Factor (280 nm) 0.777 | Extinction coefficient (cm -1 M -1) 21000 | Excitation (nm) 298 | Emission (nm) 617 |
Many biological compounds present in cells, serum or other biological fluids are naturally fluorescent, and thus the use of conventional, prompt fluorophores leads to serious limitations in assay sensitivity due to the high background caused by the autofluorescence of the biological molecules to be assayed. The use of long-lived fluorophores combined with time-resolved detection (a delay between excitation and emission detection) minimizes prompt fluorescence interferences. Our trFluor™ Eu probes enable time-resolved fluorometry (TRF) for the assays that require high sensitivity. These trFluor™ Eu probes have large Stokes shifts and extremely long emission half-lives when compared to more traditional fluorophores such as Alexa Fluor or cyanine dyes. Compared to the other TRF compounds, our trFluor™ Eu probes have relatively high stability, high emission yield and ability to be linked to biomolecules. Moreover, our trFluor™ Eu probes are insensitive to fluorescence quenching when conjugated to biological polymers such as antibodies. trFluor™ Eu DBCO *europium complex* can be readily clicked to molecules containing an azido group under copper-free conditions.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of trFluor™ Eu DBCO *europium complex* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 56.634 µL | 283.171 µL | 566.341 µL | 2.832 mL | 5.663 mL |
5 mM | 11.327 µL | 56.634 µL | 113.268 µL | 566.341 µL | 1.133 mL |
10 mM | 5.663 µL | 28.317 µL | 56.634 µL | 283.171 µL | 566.341 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.911 |
Correction Factor (280 nm) | 0.777 |
Extinction coefficient (cm -1 M -1) | 21000 |
Excitation (nm) | 298 |
Emission (nm) | 617 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
trFluor™ Eu maleimide *europium complex* | 298 | 617 | 21000 | 0.911 | 0.777 |
References
View all 49 references: Citation Explorer
Integrating Ligand and Target-Driven Based Virtual Screening Approaches With in vitro Human Cell Line Models and Time-Resolved Fluorescence Resonance Energy Transfer Assay to Identify Novel Hit Compounds Against BCL-2.
Authors: Tutumlu, Gurbet and Dogan, Berna and Avsar, Timucin and Orhan, Muge Didem and Calis, Seyma and Durdagi, Serdar
Journal: Frontiers in chemistry (2020): 167
Authors: Tutumlu, Gurbet and Dogan, Berna and Avsar, Timucin and Orhan, Muge Didem and Calis, Seyma and Durdagi, Serdar
Journal: Frontiers in chemistry (2020): 167
Discovery and Characterization of Peptide Inhibitors for Calcium and Integrin Binding Protein 1.
Authors: Puhl, Ana C and Bogart, Jonathan W and Haberman, Victoria A and Larson, Jacob E and Godoy, Andre S and Norris-Drouin, Jacqueline L and Cholensky, Stephanie H and Leisner, Tina M and Frye, Stephen V and Parise, Leslie V and Bowers, Albert A and Pearce, Kenneth H
Journal: ACS chemical biology (2020)
Authors: Puhl, Ana C and Bogart, Jonathan W and Haberman, Victoria A and Larson, Jacob E and Godoy, Andre S and Norris-Drouin, Jacqueline L and Cholensky, Stephanie H and Leisner, Tina M and Frye, Stephen V and Parise, Leslie V and Bowers, Albert A and Pearce, Kenneth H
Journal: ACS chemical biology (2020)
Comparative Analysis of High-Throughput Assays of Family-1 Plant Glycosyltransferases.
Authors: McGraphery, Kate and Schwab, Wilfried
Journal: International journal of molecular sciences (2020)
Authors: McGraphery, Kate and Schwab, Wilfried
Journal: International journal of molecular sciences (2020)
Revisit ligand-receptor interaction at the human vasopressin V2 receptor: A kinetic perspective.
Authors: Liu, Chunji and Xia, Leyi and Fu, Kequan and Cao, Xudong and Yan, Wenzhong and Cheng, Jianjun and Roux, Thomas and Peletier, Lambertus A and Yin, Xiaoxing and Guo, Dong
Journal: European journal of pharmacology (2020): 173157
Authors: Liu, Chunji and Xia, Leyi and Fu, Kequan and Cao, Xudong and Yan, Wenzhong and Cheng, Jianjun and Roux, Thomas and Peletier, Lambertus A and Yin, Xiaoxing and Guo, Dong
Journal: European journal of pharmacology (2020): 173157
LFRET, a novel rapid assay for anti-tissue transglutaminase antibody detection.
Authors: Rusanen, Juuso and Toivonen, Anne and Hepojoki, Jussi and Hepojoki, Satu and Arikoski, Pekka and Heikkinen, Markku and Vaarala, Outi and Ilonen, Jorma and Hedman, Klaus
Journal: PloS one (2019): e0225851
Authors: Rusanen, Juuso and Toivonen, Anne and Hepojoki, Jussi and Hepojoki, Satu and Arikoski, Pekka and Heikkinen, Markku and Vaarala, Outi and Ilonen, Jorma and Hedman, Klaus
Journal: PloS one (2019): e0225851
A high-throughput screen of pharmacologically active compounds for inhibitors of UHRF1 reveals epigenetic activity of anthracycline derivative chemotherapeutic drugs.
Authors: Giovinazzo, Hugh and Walker, David and Wyhs, Nicolas and Liu, Jianyong and Esopi, David M and Vaghasia, Ajay M and Jain, Yash and Bhamidipati, Akshay and Zhou, Jianya and Nelson, William G and Yegnasubramanian, Srinivasan
Journal: Oncotarget (2019): 3040-3050
Authors: Giovinazzo, Hugh and Walker, David and Wyhs, Nicolas and Liu, Jianyong and Esopi, David M and Vaghasia, Ajay M and Jain, Yash and Bhamidipati, Akshay and Zhou, Jianya and Nelson, William G and Yegnasubramanian, Srinivasan
Journal: Oncotarget (2019): 3040-3050
A General TR-FRET Assay Platform for High-Throughput Screening and Characterizing Inhibitors of Methyl-Lysine Reader Proteins.
Authors: Rectenwald, Justin M and Hardy, P Brian and Norris-Drouin, Jacqueline L and Cholensky, Stephanie H and James, Lindsey I and Frye, Stephen V and Pearce, Kenneth H
Journal: SLAS discovery : advancing life sciences R & D (2019): 693-700
Authors: Rectenwald, Justin M and Hardy, P Brian and Norris-Drouin, Jacqueline L and Cholensky, Stephanie H and James, Lindsey I and Frye, Stephen V and Pearce, Kenneth H
Journal: SLAS discovery : advancing life sciences R & D (2019): 693-700
Development of a High-Throughput Cul3-Keap1 Time-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Assay for Identifying Nrf2 Activators.
Authors: Poore, Derek D and Hofmann, Glenn and Wolfe, Lawrence A and Qi, Hongwei and Jiang, Ming and Fischer, Michael and Wu, Zining and Sweitzer, Thomas D and Chakravorty, Subhas and Donovan, Brian and Li, Hu
Journal: SLAS discovery : advancing life sciences R & D (2019): 175-189
Authors: Poore, Derek D and Hofmann, Glenn and Wolfe, Lawrence A and Qi, Hongwei and Jiang, Ming and Fischer, Michael and Wu, Zining and Sweitzer, Thomas D and Chakravorty, Subhas and Donovan, Brian and Li, Hu
Journal: SLAS discovery : advancing life sciences R & D (2019): 175-189
Quantitative high-throughput screening assays for the discovery and development of SIRPα-CD47 interaction inhibitors.
Authors: Miller, Thomas W and Amason, Joshua D and Garcin, Elsa D and Lamy, Laurence and Dranchak, Patricia K and Macarthur, Ryan and Braisted, John and Rubin, Jeffrey S and Burgess, Teresa L and Farrell, Catherine L and Roberts, David D and Inglese, James
Journal: PloS one (2019): e0218897
Authors: Miller, Thomas W and Amason, Joshua D and Garcin, Elsa D and Lamy, Laurence and Dranchak, Patricia K and Macarthur, Ryan and Braisted, John and Rubin, Jeffrey S and Burgess, Teresa L and Farrell, Catherine L and Roberts, David D and Inglese, James
Journal: PloS one (2019): e0218897
High-throughput screen, using time-resolved FRET, yields actin-binding compounds that modulate actin-myosin structure and function.
Authors: Guhathakurta, Piyali and Prochniewicz, Ewa and Grant, Benjamin D and Peterson, Kurt C and Thomas, David D
Journal: The Journal of biological chemistry (2018): 12288-12298
Authors: Guhathakurta, Piyali and Prochniewicz, Ewa and Grant, Benjamin D and Peterson, Kurt C and Thomas, David D
Journal: The Journal of biological chemistry (2018): 12288-12298
Application notes
A Meta-Analysis of Common Calcium Indicators
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
FAQ
Are Cell Navigator® Cell Plasma Membrane Staining Kits suitable for cell culture medium samples?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?
Are there any calcium indicators that don't require probenecid (PBC)?