XFD488 TCO

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Physical properties

Molecular weight	856.79
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.3
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Direct upgrades

Overview SDS Protocol

Upgrade: iFluor® 488 TCO

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of XFD488 TCO to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	116.715 µL	583.574 µL	1.167 mL	5.836 mL	11.671 mL
5 mM	23.343 µL	116.715 µL	233.429 µL	1.167 mL	2.334 mL
10 mM	11.671 µL	58.357 µL	116.715 µL	583.574 µL	1.167 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.3
Correction Factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	499
Emission (nm)	520
Quantum yield	0.92¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
XFD488 azide Same Structure to Alexa Fluor™ 488 azide	499	520	71000	0.92¹	0.30	0.11
XFD488 alkyne Same Structure to Alexa Fluor™ 488 alkyne	499	520	71000	0.92¹	0.30	0.11
XFD488 Hydroxylamine Same Structure to Alexa Fluor™ 488 Hydroxylamine	499	520	71000	0.92¹	0.30	0.11
XFD488 amine Same Structure to Alexa Fluor™ 488 amine	499	520	71000	0.92¹	0.30	0.11
XFD488 Phalloidin XFD488 Same Structure to Alexa Fluor™ 488	499	520	73000	0.92¹	0.3	0.11
XFD488 acid Same Structure to Alexa Fluor™ 488 acid	499	520	71000	0.92¹	0.30	0.11
XFD488 tetrazine Same Structure to Alexa Fluor™ 488 tetrazine	499	520	71000	0.92¹	0.30	0.11
XFD488 aldehyde Same Structure to Alexa Fluor™ 488 aldehyde	499	520	71000	0.92¹	0.30	0.11
Cy3B TCO	560	571	120000¹	0.58¹	0.048	0.069

Images

Figure 1. Chemical structure for XFD488 TCO.

References

View all 50 references: Citation Explorer

Detection of Homologous Blood Transfusion in Sport Doping by Flow Cytofluorimetry: State of the Art and New Approaches to Reduce the Risk of False-Negative Results.
Authors: Donati, Francesco and de la Torre, Xavier and Pagliarosi, Sarajane and Pirri, Daniela and Prevete, Giuliana and Botrè, Francesco
Journal: Frontiers in sports and active living (2022): 808449

A fluorescent aptasensor for the detection of Aflatoxin B1 by graphene oxide mediated quenching and release of fluorescence.
Authors: Setlem, Sai Keerthana and Mondal, Bhairab and Ramlal, Shylaja
Journal: Journal of microbiological methods (2022): 106414

Introducing Cysteines into Nanobodies for Site-Specific Labeling.
Authors: Hansen, Simon Boje and Andersen, Kasper Røjkjær
Journal: Methods in molecular biology (Clifton, N.J.) (2022): 327-343

Dynamics of intracellular neonatal Fc receptor-ligand interactions in primary macrophages using biophysical fluorescence techniques.
Authors: Pannek, Andreas and Houghton, Fiona J and Verhagen, Anne M and Dower, Steven K and Hinde, Elizabeth and Gleeson, Paul A
Journal: Molecular biology of the cell (2022): ar6

Cholecystokinin-B Receptor-Targeted Nanoparticle for Imaging and Detection of Precancerous Lesions in the Pancreas.
Authors: Smith, Jill P and Cao, Hong and Edmondson, Elijah F and Dasa, Siva Sai Krishna and Stern, Stephan T
Journal: Biomolecules (2021)

Binding of cytochrome P450 27C1, a retinoid desaturase, to its accessory protein adrenodoxin.
Authors: Glass, Sarah M and Webb, Stephany N and Guengerich, F Peter
Journal: Archives of biochemistry and biophysics (2021): 109076

Peripherally Administered Botulinum Toxin Type A Localizes Bilaterally in Trigeminal Ganglia of Animal Model.
Authors: Waskitho, Arief and Yamamoto, Yumiko and Raman, Swarnalakshmi and Kano, Fumiya and Yan, Huijiao and Raju, Resmi and Afroz, Shaista and Morita, Tsuyoshi and Ikutame, Daisuke and Okura, Kazuo and Oshima, Masamitsu and Yamamoto, Akihito and Baba, Otto and Matsuka, Yoshizo
Journal: Toxins (2021)

High-Throughput Human Telomere Length Analysis at the Single-Chromosome Level by FISH Coupled with Nano-Flow Cytometry.
Authors: Gao, Kaimin and Zhou, Yingxing and Lu, Quanyi and Lu, Jingyuan and Su, Liyun and Su, Rui and Zhang, Miaomiao and Tian, Ye and Wu, Lina and Yan, Xiaomei
Journal: Analytical chemistry (2021): 9531-9540

Distribution and neurochemical characteristic of the cardiac nerve structures in the heart of chinchilla (Chinchilla laniger Molina).
Authors: Radzimirska, Malgorzata and Kuchinka, Jacek and Kuder, Tadeusz and Nowak, Elzbieta and Trybus, Wojciech and Wrobel, Grzegorz and Szczurkowski, Aleksander
Journal: Folia histochemica et cytobiologica (2021): 157-166

Pulveraven A from the fruiting bodies of Pulveroboletus ravenelii induces apoptosis in breast cancer cell via extrinsic apoptotic signaling pathway.
Authors: Lee, Dahae and Yu, Jae Sik and Ryoo, Rhim and Kim, Jin-Chul and Jang, Tae Su and Kang, Ki Sung and Kim, Ki Hyun
Journal: The Journal of antibiotics (2021): 752-757