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What does 7-AAD bind to?

Posted December 20, 2021


Cell Viability
Cell Cycle Assays
Cell Cytotoxicity
Physiological Probes
Answer

7-AAD is a membrane impermeant intercalating dye. It binds to dsDNA by intercalating between base pairs in G-C-rich regions of DNA. 

Additional resources

Dead cell discrimination with 7-amino-actinomycin D in combination with dual color immunofluorescence in single laser flow cytometry

Cell Meter™ Fluorimetric Fixed Cell Cycle Assay Kit *Red Fluorescence Optimized for Flow Cytometry*

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