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cAMP Assays
AAT Bioquest offers a comprehensive portfolio of cAMP assays designed for high-throughput screening (HTS) and GPCR research. Cyclic adenosine monophosphate (cAMP) is a critical second messenger in G protein-coupled receptor (GPCR) signaling pathways, making accurate cAMP detection essential for drug discovery and cell signaling studies. Our Screen Quest™ platform provides multiple detection formats—from traditional ELISA to homogeneous TR-FRET and live cell assays—enabling researchers to select the optimal approach for their specific research needs.
Assay Type | Detection Method | Best For | Key Products |
|---|---|---|---|
ELISA Kits | Colorimetric or Fluorimetric | Endpoint cAMP quantification, high sensitivity | Catalogs 36371, 36373 |
TR-FRET Kit | Time-Resolved Fluorescence | HTS, homogeneous no-wash format | Catalog 36379 |
Live Cell Service Packs | Real-Time Fluorescence | GPCR screening with receptor-expressing cells | Catalog 36383, 38200-38214 |
PDE Substrates | Fluorimetric | Phosphodiesterase IV activity assays | Catalog 13603 |
Building Blocks | N/A | Custom assay development, cell loading | Catalog 20300, 20340 |
Screen Quest™ ELISA cAMP Assay Kits
Screen Quest™ ELISA cAMP Assay Kits utilize a competitive immunoassay format for accurate quantification of intracellular cAMP levels. These kits employ cAMP-specific antibodies and enzyme-conjugated detection reagents to provide sensitive, reproducible measurements suitable for studying adenylyl cyclase activation in GPCR signaling pathways.
Key Features
- Competitive ELISA format provides high specificity for cAMP detection
- Multiple readout options available in colorimetric (absorbance) or fluorimetric formats
- 96-well microplate compatible optimized for standard plate readers
- Broad applicability suitable for cell lysates and tissue samples
- Wide dynamic range for accurate quantification across physiological concentrations
Fig. 1
cAMP dose response was measured with Screen Quest™ Colorimetric ELISA cAMP Assay Kit in a clear 96-well plate with a SpectraMax microplate reader. The Absorbance can be read at 405 nm (blue line), 650 nm (red line) or 740 nm (Green line), the data in figure B are from the incubation with Amplite® Green for 3 hours.
Screen Quest™ TR-FRET No Wash cAMP Assay Kit
The Screen Quest™ TR-FRET No Wash cAMP Assay Kit provides a homogeneous, mix-and-read format ideal for high-throughput screening applications. This kit utilizes time-resolved fluorescence energy transfer (TR-FRET) technology to measure cAMP levels without wash steps, dramatically increasing throughput and reducing variability in automated screening workflows.
Key Features
- Homogeneous no-wash format eliminates separation steps for faster processing
- TR-FRET detection technology minimizes background interference and improves signal-to-noise ratio
- HTS-compatible designed for 384-well and 1536-well microplate formats
- Reduced sample handling decreases well-to-well variability
- GPCR screening optimized ideal for agonist and antagonist screening campaigns
Screen Quest™ Live Cell cAMP Assay Service Packs
Screen Quest™ Live Cell cAMP Assay Service Packs provide complete, ready-to-use solutions for monitoring Gs- and Gi-mediated GPCR signaling in real time using living cells. Each service pack includes stable cell lines expressing specific receptors along with optimized assay reagents, enabling immediate screening without the need for cell line development or assay optimization.
Key Features
- Pre-validated stable cell lines expressing target GPCRs eliminate cell line development time
- Real-time cAMP monitoring captures dynamic signaling kinetics
- Complete service packs include cells, media, and detection reagents
- Versatile screening applications suitable for agonist, antagonist, and inverse agonist screening
- Multiple receptor targets available for diverse therapeutic areas
Fig. 2
Screen Quest™ Live Cell cAMP Assay Principle
cAMP Building Blocks and PDE Substrates
AAT Bioquest provides essential building blocks for custom cAMP assay development and phosphodiesterase (PDE) research. cAMP-AM is a cell-permeant cAMP analog that enables intracellular cAMP delivery without receptor activation. TAMRA-cAMP provides a fluorescent substrate for monitoring PDE IV enzyme activity in drug screening applications.
This document (01.0273.251203r1) was last updated on Thu Feb 12 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.