Screen Quest™ Colorimetric ELISA cAMP Assay Kit

Adenosine 3’, 5’ cyclic monophosphate (cAMP) is an important second messenger in intracellular signal transduction. Monitoring levels of cAMP is one of the most common ways to screen for agonists and antagonists of GPCRs. Screen Quest™ Colorimetric ELISA cAMP Assay Kit is based on the competition between HRP-labeled cAMP and non-labeled cAMP. HRP-cAMP is displaced from the HRP-cAMP/anti-cAMP antibody complex by unlabeled free cAMP. In the absence of cAMP, HRP-cAMP conjugate is bound to anti-cAMP antibody exclusively. However, the unlabeled free cAMP in the test sample competes for anti-cAMP antibody with the HRP-cAMP antibody conjugate, therefore inhibits the binding of HRP-cAMP to anti-cAMP antibody. Our Screen Quest™ Colorimetric ELISA cAMP Assay Kit provides a sensitive method for detecting adenylate cyclase activity in biochemical or cell-based assay system. Compared to other ELISA cAMP assay kits, our kit eliminates the tedious acetylation step. The kit uses Amplite® Green as a colorimetric substrate to quantify the HRP activity. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.

Protocol

COA

Catalog	Size	Price	Quantity
36370	1 plate	Price
36371	10 plates	Price

Components

Catalog 36370 (1 plate)

Component A: cAMP Standard	1 vial (33 µg)
Component B: Assay Buffer	1 bottle (20 mL)
Component C: HRP-cAMP Conjugate	1 vial
Component D: 10X Wash Solution	1 bottle (10 mL)
Component E: Cell Lysis Buffer	1 bottle (10 mL)
Component F: Anti-cAMP Ab Coated 96-Well Plate	1 plate
Component G: Amplite™ Green	1 bottle (10 mL)

Catalog 36371 (10 plates)

Component A: cAMP Standard	1 vial (33 µg)
Component B: Assay Buffer	1 bottle (100 mL)
Component C: HRP-cAMP Conjugate	5 vials
Component D: 10X Wash Solution	1 bottle (100 mL)
Component E: Cell Lysis Buffer	1 bottle (100 mL)
Component F: Anti-cAMP Ab Coated 96-Well Plate	10 plates
Component G: Amplite™ Green	1 bottle (100 mL)

Citations

View all 3 citations: Citation Explorer

Activation of P2X7 and P2Y11 purinergic receptors inhibits migration and normalizes tumor-derived endothelial cells via cAMP signaling

Authors:

Avanzato, D and Genova, T and Pla, A Fiorio and Bernardini, M and Bianco, S and Bussolati, B and Mancardi, D and Giraudo, E and Maione, F and Cassoni, P and others, undefined

Journal:

Scientific Reports (2016)

The M2 muscarinic receptors are essential for signaling in the heart left ventricle during restraint stress in mice

Authors:

Tomankova, Hana and Valuskova, Paulina and Varejkova, Eva and Rotkova, Jana and Benes, Jan and Myslivecek, Jaromir

Journal:

Stress (2015)

THE EFFECTS OF RESTRAINT STRESS ON HEART M

Authors:

Valu{\v{s}}kov{\'a}, Paulina and Tomankov{\'a}, Hana and Rotkov{\'a}, Jana and Va{\v{r}}ejkov{\'a}, Eva and Bene{\v{s}}, Jan

References

View all 132 references: Citation Explorer

cAMP-Induced Histones H3 Dephosphorylation Is Independent of PKA and MAP Kinase Activations and Correlates With mTOR Inactivation

Authors:

Rodriguez P, Rojas J.

Journal:

J Cell Biochem (2016): 741

Changes in the Arabidopsis thaliana Proteome Implicate cAMP in Biotic and Abiotic Stress Responses and Changes in Energy Metabolism

Authors:

Alqurashi M, Gehring C, Marondedze C.

Journal:

Int J Mol Sci (2016): 852

Role of the cAMP Pathway in Glucose and Lipid Metabolism

Authors:

Ravnskjaer K, Madiraju A, Montminy M.

Journal:

Handb Exp Pharmacol (2016): 29

Odor-induced cAMP production in Drosophila melanogaster olfactory sensory neurons

Authors:

Miazzi F, Hansson BS, Wicher D.

Journal:

J Exp Biol (2016): 1798

A cardiac mitochondrial cAMP signaling pathway regulates calcium accumulation, permeability transition and cell death

Authors:

Wang Z, Liu D, Varin A, Nicolas V, Courilleau D, Mateo P, Caubere C, Rouet P, Gomez AM, V and ecasteele G, Fischmeister R, Brenner C.

Journal:

Cell Death Dis (2016): e2198

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
UNSPSC	12352200

Instrument settings

Absorbance microplate reader
Absorbance	405, 650, or 740 nm
Recommended plate	Clear plate (Component F)

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Page updated on September 25, 2025