Screen Quest™ Colorimetric ELISA cAMP Assay Kit

Screen Quest™ Colorimetric ELISA cAMP Assay Kit is a competitive colorimetric ELISA assay kit for measuring cAMP levels in biochemical or cell-based assay systems.

Competitive ELISA format: Measures cAMP through competition between unlabeled sample cAMP and HRP-labeled cAMP for anti-cAMP antibody binding
Colorimetric HRP readout: Uses Amplite® Green as a colorimetric HRP substrate to quantify the bound HRP-cAMP signal
No acetylation step: Eliminates the tedious acetylation step required by some cAMP ELISA workflows
Research applications: Suitable for GPCR screening, cAMP quantitation, adenylate cyclase pathway analysis, and biochemical or cell-based signaling studies

cAMP dose response was measured with Screen Quest™ Colorimetric ELISA cAMP Assay Kit in a clear 96-well plate with a SpectraMax microplate reader. The Absorbance can be read at 405 nm (blue line), 650 nm (red line) or 740 nm (Green line), the data in figure B are from the incubation with Amplite® Green for 3 hours.

Protocol

Catalog	Size	Price	Quantity
36370	1 plate	Price
36371	10 plates	Price

Overview

Screen Quest™ Colorimetric ELISA cAMP Assay Kit is designed for measuring adenosine 3’,5’-cyclic monophosphate (cAMP), an important second messenger in intracellular signal transduction. Monitoring cAMP levels is commonly used for screening GPCR agonists and antagonists, especially for receptors coupled to adenylate cyclase-regulated signaling pathways.

The assay is based on competition between HRP-labeled cAMP and unlabeled free cAMP from the sample. In the absence of sample cAMP, HRP-cAMP binds to the anti-cAMP antibody. When unlabeled cAMP is present, it competes with HRP-cAMP for antibody binding, reducing the amount of HRP-cAMP bound in the assay. Amplite® Green is used as the colorimetric HRP substrate to generate the measurable signal, and the assay can be performed in 96-well or 384-well microplate formats. Screen Quest™ Colorimetric ELISA cAMP Assay Kit is suitable for cAMP quantitation, GPCR signaling studies, adenylate cyclase pathway analysis, and biochemical or cell-based screening assays.

Components

Catalog 36370 (1 plate)

Component A: cAMP Standard	1 vial (33 µg)
Component B: Assay Buffer	1 bottle (20 mL)
Component C: HRP-cAMP Conjugate	1 vial
Component D: 10X Wash Solution	1 bottle (10 mL)
Component E: Cell Lysis Buffer	1 bottle (10 mL)
Component F: Anti-cAMP Ab Coated 96-Well Plate	1 plate
Component G: Amplite® Green	1 bottle (10 mL)

Catalog 36371 (10 plates)

Component A: cAMP Standard	1 vial (33 µg)
Component B: Assay Buffer	1 bottle (100 mL)
Component C: HRP-cAMP Conjugate	5 vials
Component D: 10X Wash Solution	1 bottle (100 mL)
Component E: Cell Lysis Buffer	1 bottle (100 mL)
Component F: Anti-cAMP Ab Coated 96-Well Plate	10 plates
Component G: Amplite® Green	1 bottle (100 mL)

Citations

View all 3 citations: Citation Explorer

Activation of P2X7 and P2Y11 purinergic receptors inhibits migration and normalizes tumor-derived endothelial cells via cAMP signaling

Authors:

Avanzato, D and Genova, T and Pla, A Fiorio and Bernardini, M and Bianco, S and Bussolati, B and Mancardi, D and Giraudo, E and Maione, F and Cassoni, P and others, undefined

Journal:

Scientific Reports (2016)

The M2 muscarinic receptors are essential for signaling in the heart left ventricle during restraint stress in mice

Authors:

Tomankova, Hana and Valuskova, Paulina and Varejkova, Eva and Rotkova, Jana and Benes, Jan and Myslivecek, Jaromir

Journal:

Stress (2015)

THE EFFECTS OF RESTRAINT STRESS ON HEART M

Authors:

Valu{\v{s}}kov{\'a}, Paulina and Tomankov{\'a}, Hana and Rotkov{\'a}, Jana and Va{\v{r}}ejkov{\'a}, Eva and Bene{\v{s}}, Jan

References

View all 132 references: Citation Explorer

cAMP-Induced Histones H3 Dephosphorylation Is Independent of PKA and MAP Kinase Activations and Correlates With mTOR Inactivation

Authors:

Rodriguez P, Rojas J.

Journal:

J Cell Biochem (2016): 741

Changes in the Arabidopsis thaliana Proteome Implicate cAMP in Biotic and Abiotic Stress Responses and Changes in Energy Metabolism

Authors:

Alqurashi M, Gehring C, Marondedze C.

Journal:

Int J Mol Sci (2016): 852

Role of the cAMP Pathway in Glucose and Lipid Metabolism

Authors:

Ravnskjaer K, Madiraju A, Montminy M.

Journal:

Handb Exp Pharmacol (2016): 29

Odor-induced cAMP production in Drosophila melanogaster olfactory sensory neurons

Authors:

Miazzi F, Hansson BS, Wicher D.

Journal:

J Exp Biol (2016): 1798

A cardiac mitochondrial cAMP signaling pathway regulates calcium accumulation, permeability transition and cell death

Authors:

Wang Z, Liu D, Varin A, Nicolas V, Courilleau D, Mateo P, Caubere C, Rouet P, Gomez AM, V and ecasteele G, Fischmeister R, Brenner C.

Journal:

Cell Death Dis (2016): e2198

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	405, 650, or 740 nm
Recommended plate	Clear plate (Component F)

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Fax
Email	sales@aatbio.com
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Page updated on October 3, 2026