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Cell Culture and Lysis
AAT Bioquest offers a comprehensive selection of cell culture and lysis reagents designed to support critical steps in sample preparation workflows. This category includes ready-to-use lysis buffers optimized for different cell types (mammalian, bacterial, yeast, viral), specialized cell culture additives, fixation solutions, and post-processing reagents. Our ReadiUse™ and ReadiPrep™ product lines emphasize convenience and reliability, requiring minimal preparation time while delivering consistent, reproducible results across applications including protein extraction, flow cytometry, fluorescence imaging, and immunological studies.
Product Group | Example Applications | Useful For | Example Products |
|---|---|---|---|
Cell Culture Reagents | Media additives, pH control | Culture maintenance, dye loading | HHBS, Phenol Red, Pluronic® F-127 |
Cell Detachment | Cell harvest, passaging | Animal-free detachment, trypsin alternative | ReadiUse™ Cell Detaching Buffer |
Lysis and Fixation | Protein/nucleic acid extraction, cell preservation | Mammalian, bacterial, yeast, viral samples | ReadiUse™ Lysis Buffers, Formaldehyde |
Cell Culture Reagents
Cell culture reagents form the foundation of any cell biology workflow. This product group includes essential buffers, pH indicators, and surfactants used to maintain cell viability, support media formulation, and enhance the cellular uptake of hydrophobic dyes. These products are critical for establishing optimal culture conditions before cell detachment, fixation, or lysis steps.
Key Features
- HHBS (Hanks' Buffer with HEPES) – Balanced salt solution with buffering capacity for maintaining cell culture pH
- Phenol Red sodium salt – pH indicator for monitoring culture media conditions
- Pluronic® F-127 – Non-toxic surfactant that improves AM ester dye loading efficiency
- Multiple formats available – Powder (cell culture tested) or 10% aqueous solution for flexibility
Cell Detachment
Cell detachment is a critical step for cell harvesting and passaging in culture workflows. ReadiUse™ Cell Detaching Buffer provides a gentle, animal-free alternative to enzymatic methods like trypsinization. This buffer preserves cell viability and surface marker integrity, making it ideal for downstream applications including flow cytometry and immunological assays.
Key Features
- ReadiUse™ Cell Detaching Buffer – Animal-free alternative to trypsin for gentle cell detachment
- Preserves cell viability – Maintains morphology and marker expression for sensitive applications
- Ready-to-use format – No activation or dilution required; apply directly to cells
- Compatible with all downstream applications – Flow cytometry, culture continuation, immunological assays
Lysis and Fixation
Lysis and fixation form the core of sample preparation for protein extraction, nucleic acid isolation, and immunological studies. This product group includes formaldehyde-based fixation solutions for cell preservation and comprehensive lysis buffers optimized for specific cell types (mammalian, bacterial, yeast, viral). The 5X concentrated format allows simple dilution while maintaining efficacy.
Key Features
- ReadiUse™ 4% formaldehyde – Pre-diluted, ready-to-apply fixation solution for consistent results
- Cell-type specific lysis buffers – Optimized formulations for mammalian, bacterial, yeast, or viral samples
- Gentle yet effective – Preserves protein integrity and enzymatic activity for downstream assays
- Compatible with downstream applications – Western blot, ELISA, enzymatic assays, mass spectrometry, flow cytometry
Fig. 1
F-actin stain of CPA cells. CPA cells were fixed with ReadiUse™ 4% formaldehyde fixation solution (Catalog Number 20010) and then stained with Cell Navigator® F-Actin Labeling Kit (Catalog Number 22661). A: label with 1X iFluor® 488-phalloidin for 30 min only. B: Treat the cells with phalloidin for 10 min, then stain them with 1X iFluor® 488-phalloidin for 30 min.
RBC Lysis and Blood Processing
ReadiUse™ RBC (Red Blood Cell) lysis solutions are specifically formulated for processing whole blood samples. These ammonium chloride-based solutions selectively lyse erythrocytes while preserving leukocyte viability and morphology, making them ideal for flow cytometry and immunophenotyping applications.
Key Features
- Selective RBC lysis – Efficiently removes red blood cells while preserving white blood cells
- Leukocyte-friendly – Maintains cell viability and surface marker integrity
- Dual-function option – Lysis/Fixation solution combines RBC removal with simultaneous fixation
- Phosphorylation preservation – Lysis/Fixation solution maintains phospho-epitopes for signaling studies
This document (01.0335.251203r1) was last updated on Sat Feb 28 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.
Cell Culture and Lysis