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Galactosidase
AAT Bioquest offers a comprehensive selection of galactosidase substrates and assay kits for detecting β-galactosidase activity. These products span fluorimetric, colorimetric, and bioluminescent detection methods, supporting applications from basic enzyme kinetics to cellular senescence research. Our Amplite® assay kits provide ready-to-use solutions for high-throughput screening, while individual substrates offer flexibility for custom protocols.
Product Category | Detection Method | Best For | Key Products |
|---|---|---|---|
Amplite® Assay Kits | Fluorimetric / Colorimetric | High-throughput β-gal quantification in lysates and supernatants | Amplite® Green, Red, Colorimetric Kits |
Cell Meter™ Senescence Kits | Fluorimetric / Colorimetric | SA-β-gal detection in live cells (flow cytometry, imaging) | Cell Meter™ Green, Red, Blue Kits |
Xite™ Fluorogenic Substrates | Fluorimetric (Green / Red) | Live cell β-gal with superior signal-to-background | Xite™ Green, Xite™ Red |
FDG, MUP & Other Fluorogenic Substrates | Fluorimetric (UV / Green / Red) | Custom protocols, enzyme kinetics, alpha- and β-galactosidase | FDG, MUG, CUG, Resorufin substrates |
D-Luciferin Galactoside | Bioluminescent | Reporter gene assays, dual-reporter systems | D-Luciferin galactoside |
Amplite® β-galactosidase Assay Kits
Amplite® assay kits provide complete, optimized solutions for quantifying β-galactosidase activity in cell lysates, purified enzyme preparations, or culture supernatants. Each kit includes substrate, buffer, and all necessary reagents for microplate-based detection, enabling sensitive and reproducible measurements.
Key Features
- Ready-to-use formulations — Pre-optimized buffers eliminate protocol development time
- High sensitivity — Detect β-galactosidase at sub-nanogram levels
- Multiple detection options — Choose green fluorescence, red fluorescence, or colorimetric readout based on instrumentation
- Microplate compatible — Designed for 96-well and 384-well high-throughput screening
Fig. 1
β -galactosidase dose response was measured with Amplite® Colorimetric β-Galactosidase Assay Kit in a 96-well clear bottom plate using a SpectraMax microplate reader (Molecular Devices). As low as 3 mU/mL β- galactosidase was detected with 30-60 minutes incubation. Note: The absorbance background increases with time, thus it is important to subtract the absorbance of the blank wells for each data point.
Cell Meter™ Senescence-Associated β-galactosidase Assay Kits
Cell Meter™ Senescence Assay Kits enable direct detection of senescence-associated β-galactosidase (SA-beta-gal) activity in live cells. SA-beta-gal is a well-established biomarker of cellular senescence, an irreversible growth arrest state triggered by DNA damage, telomere shortening, or oncogenic stress. These kits are optimized for flow cytometry and fluorescence microscopy applications.
Key Features
- Live cell compatible — Detect SA-beta-gal activity without cell fixation or permeabilization
- Flow cytometry and imaging ready — Optimized for both platforms
- Multiple color options — Green, red, and colorimetric formats for experimental flexibility
- Drug-induced senescence detection — Validated for chemotherapy-induced senescence studies
β-galactosidase Substrates
Xite™ Fluorogenic Beta-D-Galactopyranosides
Xite™ beta-D-galactopyranosides are fluorogenic probes designed for β-galactosidase detection with improved fluorescence signal characteristics and cell permeability compared to commonly used substrates. These substrates deliver superior signal-to-background ratios compared to traditional substrates like FDG, with enhanced brightness and improved cell permeability for live cell applications.
Key Features
- Higher signal-to-background — Outperforms FDG and other traditional substrates in comparative studies
- Optimized for live cells — Excellent cell permeability without hypotonic loading
- Flow cytometry and microscopy compatible — Validated for both platforms
- Green and red options — Choose based on instrumentation or multiplexing needs
FDG, MUP and Other Fluorogenic Substrates
Classic fluorogenic substrates, such as FDG and MUP, provide additional flexibility for researchers developing custom assay protocols or requiring specific spectral properties. These substrates release fluorescent products upon enzymatic cleavage, enabling real-time monitoring of galactosidase activity. Products are available for both alpha- and β-galactosidase detection across ultraviolet, green, and red emission wavelengths.
D-Luciferin Galactoside
D-Luciferin galactoside enables bioluminescent detection of β-galactosidase activity when used in conjunction with firefly luciferase. The beta-galactoside moiety blocks luciferase recognition; upon enzymatic cleavage, free D-luciferin is released and can be quantified through luminescence. This approach is particularly useful for reporter gene assays where luciferase and lacZ are used as dual reporters.
This document (01.0259.251203r1) was last updated on Sat Feb 28 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.