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RNA Quantification
AAT Bioquest offers a comprehensive range of RNA quantification products designed for accurate detection and measurement of RNA in various research applications. From fluorimetric quantification kits optimized for different instrument platforms to live cell RNA imaging solutions, these products provide researchers with reliable tools for measuring yields from in vitro transcription, assessing RNA quality before downstream applications, and visualizing RNA distribution in living cells.
Product Type | Features | Platform | Best For |
|---|---|---|---|
Helixyte™ Kits | Sensitivity of 5-100 ng (HS) or 20-1000 ng (BR) | Microplate readers | High-throughput RNA quantification |
Portelite™ Kits | Sensitivity of 5-100 ng (HS) or 20-1000 ng (BR) | Cytocite™/Qubit™ fluorometers | Fast, single-tube quantification |
StrandBrite™ Kits | High selectivity | Microplate readers | RNA-selective detection (minimal DNA interference) |
Cell Navigator® Kit | Live cell compatible | Fluorescence microscopy | Live cell RNA visualization |
RNA-Binding Dyes | Various | Various | Custom staining protocols |
Fluorimetric RNA Quantification Kits
AAT Bioquest offers multiple fluorimetric RNA quantification kit lines optimized for different instrument platforms and sensitivity requirements. These kits utilize fluorescent dyes that exhibit dramatic fluorescence enhancement upon binding to RNA, enabling sensitive and accurate quantification across a wide dynamic range.
Key Features
- Dramatic signal enhancement upon RNA binding for optimal sensitivity
- Broad dynamic ranges accommodating diverse sample concentrations
- Optimized chemistry for multiple instrument platforms
- Minimal sample preparation required for rapid quantification
Fig. 1
RNA dose response was measured with Helixyte™ Fluorimetric RNA Quantitation Assay Kit *High Sensitivity* in a 96-well solid black plate.
Helixyte™ RNA Quantification Kits
Helixyte™ Fluorimetric RNA Quantification Kits are designed for accurate, high-throughput RNA detection using standard microplate readers. Available in both high sensitivity (5-100 ng) and broad range (20-1000 ng) formats, these kits provide flexibility for quantifying RNA from various sample types.
Key Features
- 96-well and 384-well compatible for microplate reader formats
- High sensitivity option detects as little as 5 ng RNA
- Broad range option covers 20-1000 ng for typical sample concentrations
- Simple mix-and-read protocol with minimal sample handling
- Green fluorescence output compatible with standard FITC/GFP filter sets
Portelite™ RNA Quantification Kits
Portelite™ Fluorimetric RNA Quantification Kits are specifically optimized for use with benchtop fluorometers including the Cytocite™ and Qubit™ platforms. These kits provide accurate RNA measurements with the convenience of single tube testing and minimal sample volume requirements.
Key Features
- Optimized for Cytocite™ and Qubit™ fluorometer compatibility
- Pre-optimized assay conditions for fast RNA quantification
- High sensitivity (5-100 ng) and broad range (20-1000 ng) formats available
- Rapid quantification with minimal sample volume requirements
- Total nucleic acid quantification option also available
StrandBrite™ RNA Quantification Kits & Reagents
StrandBrite™ products are specifically designed for RNA-selective quantification with minimal interference from DNA. The High Selectivity kit addresses a major challenge in RNA analysis—minimizing DNA interference—making it ideal for samples containing mixed nucleic acids. These products are optimized for microplate reader formats and provide excellent performance in high-throughput applications.
Key Features
- High selectivity option specifically designed to minimize DNA interference
- Ideal for mixed nucleic acid samples containing both DNA and RNA
- Green fluorescence output (Ex/Em = 509/527 nm) for standard filter sets
- Available as complete kits or standalone reagent for custom protocols
- Microplate reader-optimized format for high-throughput applications
Live Cell RNA Imaging
The Cell Navigator® Live Cell RNA Imaging Kit enables direct visualization of RNA molecules within living cells using fluorescence microscopy. This kit is essential for studying RNA localization, transport, and dynamics in real-time without cell fixation. The intuitive labeling approach makes it ideal for researchers investigating cellular RNA distribution and subcellular RNA trafficking patterns.
Key Features
- Live cell imaging without cell fixation or permeabilization
- Green fluorescence output (Ex/Em = 509/527 nm) compatible with standard filter sets
- Real-time visualization of RNA localization and transport
- Multicolor imaging compatible when combined with other cellular stains
- Standard fluorescence microscopy platform compatible
Fig. 2
Fluorescence images of RNA staining in HeLa cells. (A) Live cells were stained using Cell Navigator® Live Cell RNA Imaging Kit (Green, Catalog Number 22630) and counter-stained with Hoechst 33342 (Blue, Catalog Number 17530). (B) Cells fixed in methanol were stained using the same kit. (C) After staining, fixed HeLa cells were incubated with 0.5 mg/mL RNase at 37 ºC for 1 hour. Image of RNase digest test indicates the high selectivity of StrandBrite™ RNA Green. The green fluorescence signal were measured using a fluorescence microscope with a FITC filter.
RNA-Binding Dyes
For researchers requiring standalone RNA-binding dyes for custom staining protocols, AAT Bioquest offers individual dye reagents that can be incorporated into specialized workflows. These reagents provide flexibility for developing assays tailored to specific application requirements and instrumentation.
Key Features
- Individual dye reagents for custom protocol development
- Versatile applications across multiple detection platforms
- High purity chemical standards for research applications
This document (01.0304.251203r1) was last updated on Sat Feb 28 2026. All trademarks and registered trademarks mentioned herein are the property of their respective owners.