Name: Advantages and Applications of Calcium Indicators
Creator: AAT Bioquest
License: https://www.aatbio.com/tou.html

Advantages and Applications of Calcium Indicators

Calcium Indicator	Tools	Excitation Source	Advantage	Applications
Single-Wavelength	Calbryte-520 (cat. no. 20650, 20656) Fluo-8® (cat. no. 21080, 21086, 21087) Rhod-2 (cat. no. 21060) Rhod-4 (cat. no. 21120)	Visible Light	Large dynamic range excellent for detecting modest and transient Ca²⁺ fluxes	Qualitative Data Analysis Fluorescence Microscopy Flow Cytometry Calcium Imaging
Dual-Wavelength	Fura-2 (cat. no. 21020, 21025, 21026) Indo-1 (cat. no. 21030) Fura-8 (cat. no. 21055) Fura-Red (cat. no. 21045, 21046)	Ultra Violet Light (Fura-2, Indo-1, & Fura-8) Visible Light (Fura-Red)	Accurately measures Ca²⁺ concentrations. Avoids artifacts associated with variable dye distribution, photobleaching, and indicator leakage.	Quantitative Data Analysis Fluorescence Microscopy Flow Cytometry Calcium Imaging
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References

This online resource may be cited as follows

MLA	"Quest Database™ Advantages and Applications of Calcium Indicators." AAT Bioquest, Inc., 11 Jan. 2026, https://www.aatbio.com/data-sets/advantages-and-applications-of-calcium-indicators.
APA	AAT Bioquest, Inc. (2026, January 11). Quest Database™ Advantages and Applications of Calcium Indicators. AAT Bioquest. https://www.aatbio.com/data-sets/advantages-and-applications-of-calcium-indicators.
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