Helix Fluor™ 575, succinimidyl ester
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 770.31 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 553 |
Emission (nm) | 570 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Related products
Helix Fluor™ 6-JOE Phosphoramidite |
Overview | SDSProtocol |
See also: Amine Reactive Dyes and Probes for Conjugation, Peptide and Oligonucleotide Labeling, Digital PCR, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), RNA Purification & Analysis, PCR Detection of Viral DNA/RNA
Molecular weight 770.31 | Excitation (nm) 553 | Emission (nm) 570 |
FAM, TET, VIC, JOE, NED, TAMRA and ROX fluorescent dyes are frequently used for labeling oliogs. These fluorescent oligo conjugates are often used for nucleic acid sequencing and nucleic acid amplification. Our Helix Fluor™ 575 is an excellent alternative to the NED dye for preparing fluorescent oligos.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Helix Fluor™ 575, succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 129.818 µL | 649.089 µL | 1.298 mL | 6.491 mL | 12.982 mL |
5 mM | 25.964 µL | 129.818 µL | 259.636 µL | 1.298 mL | 2.596 mL |
10 mM | 12.982 µL | 64.909 µL | 129.818 µL | 649.089 µL | 1.298 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Images
Figure 1. Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide or nucleic acid. NHS esters react readily with the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates are quite stable.
Citations
View all 1 citations: Citation Explorer
Synthesis of Phytosulfokine Analogs as Probes for Studying Plant Signaling and Molecular Trafficking
Authors: Ntim, Thomas
Journal: (2021)
Authors: Ntim, Thomas
Journal: (2021)
References
View all 1 references: Citation Explorer
Detection and typing of Herpes Simplex virus (HSV) in mucocutaneous samples by TaqMan PCR targeting a gB segment homologous for HSV types 1 and 2
Authors: Namvar L, Olofsson S, Bergstrom T, Lindh M.
Journal: J Clin Microbiol (2005): 2058
Authors: Namvar L, Olofsson S, Bergstrom T, Lindh M.
Journal: J Clin Microbiol (2005): 2058