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5-Carboxyrhodamine 6G maleimide

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Physical properties
Molecular weight765.74
Spectral properties
Absorbance (nm)530
Extinction coefficient (cm -1 M -1)1160001
Excitation (nm)525
Emission (nm)548
Quantum yield0.951
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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5-CR6G [5-Carboxyrhodamine 6G] *Single isomer*
6-CR6G [6-Carboxyrhodamine 6G] *Single isomer*
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5(6)-Carboxyrhodamine 6G cadaverine
5(6)-Carboxyrhodamine 6G ethylenediamine
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Phalloidin-Tetramethylrhodamine Conjugate
5-dR6G [5-Carboxy-4,7-dichlororhodamine 6G]
6-dR6G [6-Carboxy-4,7-dichlororhodamine 6G]
5-dR110 [5-Carboxy-4,7-dichlororhodamine 110]
6-dR110 [6-Carboxy-4,7-dichlororhodamine 110]
Bis-L-Aspartic acid Beta-rhodamine 110
Bis-Arg-rhodamine 110
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Rhodamine aldehyde [5-TAMRA aldehyde]
Show More (47)


Molecular weight
Absorbance (nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
5-CR6G maleimide is a thiol-reactive derivative of rhodamine 6G. It is preferred choice (than the mixed isomers) for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-CR6G and 6-CR6G might significantly affect some biological properties of the underlying conjugates. Rhodamine 6G is one of the brightest rhodamine dyes, but most of the rhodamine 6G dyes have much lower solubility than other rhodamine dyes. For similar or equivalent spectral properties, iFluor dyes have much better water solubilities.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-Carboxyrhodamine 6G maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM130.593 µL652.963 µL1.306 mL6.53 mL13.059 mL
5 mM26.119 µL130.593 µL261.185 µL1.306 mL2.612 mL
10 mM13.059 µL65.296 µL130.593 µL652.963 µL1.306 mL

Molarity calculator

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Spectral properties

Absorbance (nm)530
Extinction coefficient (cm -1 M -1)1160001
Excitation (nm)525
Emission (nm)548
Quantum yield0.951



View all 50 references: Citation Explorer
Schwann Cell Migration through Magnetic Actuation Mediated by Fluorescent-Magnetic Bifunctional Fe3O4·Rhodamine 6G@Polydopamine Superparticles.
Authors: Wang, Yang and Du, Shulin and Liu, Ting and Ren, Jingyan and Zhang, Jiayi and Xu, Hao and Zhang, Hao and Liu, Yi and Lu, Laijin
Journal: ACS chemical neuroscience (2020): 1359-1370
Graphene-like BN@SiO2 nanocomposites as efficient sorbents for solid-phase extraction of Rhodamine B and Rhodamine 6G from food samples.
Authors: Chao, Yanhong and Pang, Jingyu and Bai, Yan and Wu, Peiwen and Luo, Jing and He, Jing and Jin, Yan and Li, Xiaowei and Xiong, Jun and Li, Huaming and Zhu, Wenshuai
Journal: Food chemistry (2020): 126666
Development of Rhodamine 6G-Based Fluorescent Chemosensors for Al3+-Ion Detection: Effect of Ring Strain and Substituent in Enhancing Its Sensing Performance.
Authors: Mandal, Jayanta and Ghorai, Pravat and Pal, Kunal and Bhaumik, Tanurima and Karmakar, Parimal and Saha, Amrita
Journal: ACS omega (2020): 145-157
A Fluorescent and Colorimetric Chemosensor for Hg2+ Based on Rhodamine 6G With a Two-Step Reaction Mechanism.
Authors: Bai, Cui-Bing and Wang, Wei-Gang and Zhang, Jie and Wang, Chang and Qiao, Rui and Wei, Biao and Zhang, Lin and Chen, Shui-Sheng and Yang, Song
Journal: Frontiers in chemistry (2020): 14
Growth and elongation of axons through mechanical tension mediated by fluorescent-magnetic bifunctional Fe3O4·Rhodamine 6G@PDA superparticles.
Authors: Wang, Yang and Li, Binxi and Xu, Hao and Du, Shulin and Liu, Ting and Ren, Jingyan and Zhang, Jiayi and Zhang, Hao and Liu, Yi and Lu, Laijin
Journal: Journal of nanobiotechnology (2020): 64
Application of Doehlert Matrix for an Optimized Preparation of a Surface-Enhanced Raman Spectroscopy (SERS) Substrate Based on Silicon Nanowires for Ultrasensitive Detection of Rhodamine 6G.
Authors: Ouhibi, Awatef and Saadaoui, Maroua and Lorrain, Nathalie and Guendouz, Mohammed and Raouafi, Noureddine and Moadhen, Adel
Journal: Applied spectroscopy (2020): 168-177
Removal of rhodamine 6G from synthetic effluents using Clitoria fairchildiana pods as low-cost biosorbent.
Authors: da Silva, Andreza Miranda Barata and Serrão, Naiany Oliveira and de Gusmão Celestino, Gustavo and Takeno, Mitsuo Lopes and Antunes, Nicanor Tiago Bueno and Iglauer, Stefan and Manzato, Lizandro and de Freitas, Flávio Augusto and Maia, Paulo José Sousa
Journal: Environmental science and pollution research international (2020): 2868-2880
Ultrasensitive SERS detection of rhodamine 6G and p-nitrophenol based on electrochemically roughened nano-Au film.
Authors: Wang, Jiangcai and Qiu, Cuicui and Mu, Xijiao and Pang, Hua and Chen, Xinchun and Liu, Dameng
Journal: Talanta (2020): 120631
Two-Photon-Induced Fluorescence Study of Rhodamine-6G Dye in Different Sets of Binary Solvents.
Authors: Gupta, Rahul Kumar and Kant, Surya and Rawat, Ashwini Kumar and Goswami, Debabrata
Journal: Journal of fluorescence (2020): 1043-1048
Photocatalytic Degradation of Aqueous Rhodamine 6G Using Supported TiO2 Catalysts. A Model for the Removal of Organic Contaminants From Aqueous Samples.
Authors: Pino, Eduardo and Calderón, Cristian and Herrera, Francisco and Cifuentes, Gerardo and Arteaga, Gisselle
Journal: Frontiers in chemistry (2020): 365