5-Carboxyrhodamine 6G maleimide

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Physical properties

Molecular weight	765.74
Solvent	DMSO

Spectral properties

Absorbance (nm)	530
Extinction coefficient (cm ^-1 M ^-1)	116000¹
Excitation (nm)	525
Emission (nm)	548
Quantum yield	0.95¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

765.74

Absorbance (nm)

530

Extinction coefficient (cm ^-1 M ^-1)

116000¹

Excitation (nm)

525

Emission (nm)

548

Quantum yield

0.95¹

5-CR6G maleimide is a thiol-reactive derivative of rhodamine 6G. It is preferred choice (than the mixed isomers) for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-CR6G and 6-CR6G might significantly affect some biological properties of the underlying conjugates. Rhodamine 6G is one of the brightest rhodamine dyes, but most of the rhodamine 6G dyes have much lower solubility than other rhodamine dyes. For similar or equivalent spectral properties, iFluor dyes have much better water solubilities.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-Carboxyrhodamine 6G maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	130.593 µL	652.963 µL	1.306 mL	6.53 mL	13.059 mL
5 mM	26.119 µL	130.593 µL	261.185 µL	1.306 mL	2.612 mL
10 mM	13.059 µL	65.296 µL	130.593 µL	652.963 µL	1.306 mL

Molarity calculator

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Spectrum

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Spectral properties

Absorbance (nm)	530
Extinction coefficient (cm ^-1 M ^-1)	116000¹
Excitation (nm)	525
Emission (nm)	548
Quantum yield	0.95¹

Images

Figure 1. Chemical structure for 5-Caroxyrhodamine 6G maleimide.

References

View all 50 references: Citation Explorer

Schwann Cell Migration through Magnetic Actuation Mediated by Fluorescent-Magnetic Bifunctional Fe3O4·Rhodamine 6G@Polydopamine Superparticles.
Authors: Wang, Yang and Du, Shulin and Liu, Ting and Ren, Jingyan and Zhang, Jiayi and Xu, Hao and Zhang, Hao and Liu, Yi and Lu, Laijin
Journal: ACS chemical neuroscience (2020): 1359-1370

Sandwich-Like Sensor for the Highly Specific and Reproducible Detection of Rhodamine 6G on a Surface-Enhanced Raman Scattering Platform.
Authors: Wang, Jing and Li, Jingyi and Zeng, Chuan and Qu, Qi and Wang, Mengfan and Qi, Wei and Su, Rongxin and He, Zhimin
Journal: ACS applied materials & interfaces (2020): 4699-4706

PANI/PbS QD nanocomposite structure for visible light driven photocatalytic degradation of rhodamine 6G.
Authors: Chhabra, Varun A and Kaur, Rajnish and Walia, Manrajvir S and Kim, Ki-Hyun and Deep, Akash
Journal: Environmental research (2020): 109615

Quantification of mitochondrial membrane potential in the isolated rat lung using rhodamine 6G.
Authors: Audi, Said H and Cammarata, Anthony and Clough, Anne V and Dash, Ranjan K and Jacobs, Elizabeth R
Journal: Journal of applied physiology (Bethesda, Md. : 1985) (2020): 892-906

Adsorption of Rhodamine 6G Dye on Binary System of Nanoarchitectonics Composite Magnetic Graphene Oxide Material.
Authors: Gautam, Drashya and Lal, Shyam and Hooda, Sunita
Journal: Journal of nanoscience and nanotechnology (2020): 2939-2945

Flow-injection determination of manganese (II) using surfactant enhanced diperiodatonickelate (IV)-rhodamine 6G chemiluminescence.
Authors: Asghar, Muhammad and Yaqoob, Mohammad and Siddiqui, Masood Ahmed and Munawar, Nusrat and Waseem, Amir and Nabi, Abdul
Journal: Luminescence : the journal of biological and chemical luminescence (2020): 79-89

Consecutive Photoinduced Electron Transfer (conPET): The Mechanism of the Photocatalyst Rhodamine 6G.
Authors: Brandl, Fabian and Bergwinkl, Sebastian and Allacher, Carina and Dick, Bernhard
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 7946-7954

Photocatalytic Degradation of Aqueous Rhodamine 6G Using Supported TiO2 Catalysts. A Model for the Removal of Organic Contaminants From Aqueous Samples.
Authors: Pino, Eduardo and Calderón, Cristian and Herrera, Francisco and Cifuentes, Gerardo and Arteaga, Gisselle
Journal: Frontiers in chemistry (2020): 365

Two-Photon-Induced Fluorescence Study of Rhodamine-6G Dye in Different Sets of Binary Solvents.
Authors: Gupta, Rahul Kumar and Kant, Surya and Rawat, Ashwini Kumar and Goswami, Debabrata
Journal: Journal of fluorescence (2020): 1043-1048

Ultrasensitive SERS detection of rhodamine 6G and p-nitrophenol based on electrochemically roughened nano-Au film.
Authors: Wang, Jiangcai and Qiu, Cuicui and Mu, Xijiao and Pang, Hua and Chen, Xinchun and Liu, Dameng
Journal: Talanta (2020): 120631