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5-Carboxyrhodamine 6G maleimide

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Physical properties
Molecular weight765.74
Spectral properties
Absorbance (nm)530
Extinction coefficient (cm -1 M -1)1160001
Excitation (nm)525
Emission (nm)548
Quantum yield0.951
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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5-CR6G [5-Carboxyrhodamine 6G] *Single isomer*
6-CR6G [6-Carboxyrhodamine 6G] *Single isomer*
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5(6)-Carboxyrhodamine 6G cadaverine
5(6)-Carboxyrhodamine 6G ethylenediamine
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Phalloidin-Tetramethylrhodamine Conjugate
5-dR6G [5-Carboxy-4,7-dichlororhodamine 6G]
6-dR6G [6-Carboxy-4,7-dichlororhodamine 6G]
5-dR110 [5-Carboxy-4,7-dichlororhodamine 110]
6-dR110 [6-Carboxy-4,7-dichlororhodamine 110]
Bis-L-Aspartic acid Beta-rhodamine 110
Bis-Arg-rhodamine 110
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Rhodamine aldehyde [5-TAMRA aldehyde]
Show More (47)


Molecular weight
Absorbance (nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
5-CR6G maleimide is a thiol-reactive derivative of rhodamine 6G. It is preferred choice (than the mixed isomers) for some complicated biological applications where reproducibility is more critical than material cost since the minor positional difference between 5-CR6G and 6-CR6G might significantly affect some biological properties of the underlying conjugates. Rhodamine 6G is one of the brightest rhodamine dyes, but most of the rhodamine 6G dyes have much lower solubility than other rhodamine dyes. For similar or equivalent spectral properties, iFluor dyes have much better water solubilities.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-Carboxyrhodamine 6G maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM130.593 µL652.963 µL1.306 mL6.53 mL13.059 mL
5 mM26.119 µL130.593 µL261.185 µL1.306 mL2.612 mL
10 mM13.059 µL65.296 µL130.593 µL652.963 µL1.306 mL

Molarity calculator

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Spectral properties

Absorbance (nm)530
Extinction coefficient (cm -1 M -1)1160001
Excitation (nm)525
Emission (nm)548
Quantum yield0.951



View all 50 references: Citation Explorer
Schwann Cell Migration through Magnetic Actuation Mediated by Fluorescent-Magnetic Bifunctional Fe3O4·Rhodamine 6G@Polydopamine Superparticles.
Authors: Wang, Yang and Du, Shulin and Liu, Ting and Ren, Jingyan and Zhang, Jiayi and Xu, Hao and Zhang, Hao and Liu, Yi and Lu, Laijin
Journal: ACS chemical neuroscience (2020): 1359-1370
Sandwich-Like Sensor for the Highly Specific and Reproducible Detection of Rhodamine 6G on a Surface-Enhanced Raman Scattering Platform.
Authors: Wang, Jing and Li, Jingyi and Zeng, Chuan and Qu, Qi and Wang, Mengfan and Qi, Wei and Su, Rongxin and He, Zhimin
Journal: ACS applied materials & interfaces (2020): 4699-4706
PANI/PbS QD nanocomposite structure for visible light driven photocatalytic degradation of rhodamine 6G.
Authors: Chhabra, Varun A and Kaur, Rajnish and Walia, Manrajvir S and Kim, Ki-Hyun and Deep, Akash
Journal: Environmental research (2020): 109615
Quantification of mitochondrial membrane potential in the isolated rat lung using rhodamine 6G.
Authors: Audi, Said H and Cammarata, Anthony and Clough, Anne V and Dash, Ranjan K and Jacobs, Elizabeth R
Journal: Journal of applied physiology (Bethesda, Md. : 1985) (2020): 892-906
Adsorption of Rhodamine 6G Dye on Binary System of Nanoarchitectonics Composite Magnetic Graphene Oxide Material.
Authors: Gautam, Drashya and Lal, Shyam and Hooda, Sunita
Journal: Journal of nanoscience and nanotechnology (2020): 2939-2945
Flow-injection determination of manganese (II) using surfactant enhanced diperiodatonickelate (IV)-rhodamine 6G chemiluminescence.
Authors: Asghar, Muhammad and Yaqoob, Mohammad and Siddiqui, Masood Ahmed and Munawar, Nusrat and Waseem, Amir and Nabi, Abdul
Journal: Luminescence : the journal of biological and chemical luminescence (2020): 79-89
Consecutive Photoinduced Electron Transfer (conPET): The Mechanism of the Photocatalyst Rhodamine 6G.
Authors: Brandl, Fabian and Bergwinkl, Sebastian and Allacher, Carina and Dick, Bernhard
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020): 7946-7954
Photocatalytic Degradation of Aqueous Rhodamine 6G Using Supported TiO2 Catalysts. A Model for the Removal of Organic Contaminants From Aqueous Samples.
Authors: Pino, Eduardo and Calderón, Cristian and Herrera, Francisco and Cifuentes, Gerardo and Arteaga, Gisselle
Journal: Frontiers in chemistry (2020): 365
Two-Photon-Induced Fluorescence Study of Rhodamine-6G Dye in Different Sets of Binary Solvents.
Authors: Gupta, Rahul Kumar and Kant, Surya and Rawat, Ashwini Kumar and Goswami, Debabrata
Journal: Journal of fluorescence (2020): 1043-1048
Ultrasensitive SERS detection of rhodamine 6G and p-nitrophenol based on electrochemically roughened nano-Au film.
Authors: Wang, Jiangcai and Qiu, Cuicui and Mu, Xijiao and Pang, Hua and Chen, Xinchun and Liu, Dameng
Journal: Talanta (2020): 120631