5-FITC ethylenediamine [Fluorescein thiocarbamylethylenediamine] *CAS 75453-82-6*
5-FITC ethylenediamine is an excellent building block for developing fluoresceinated bioconjugates. It has been used for labeling peptides and small biomolecules that contain a carboxy group. It can also be used for labeling carbohydrates or glycoproteins via reductive amination.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 5-FITC ethylenediamine [Fluorescein thiocarbamylethylenediamine] *CAS 75453-82-6* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 177.462 µL | 887.311 µL | 1.775 mL | 8.873 mL | 17.746 mL |
5 mM | 35.492 µL | 177.462 µL | 354.925 µL | 1.775 mL | 3.549 mL |
10 mM | 17.746 µL | 88.731 µL | 177.462 µL | 887.311 µL | 1.775 mL |
Molarity calculator
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Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
5-FAM ethylenediamine | 493 | 517 | 83000 | 0.32 | 0.178 |
5-FITC cadaverine | 491 | 516 | 73000 | - | 0.35 |
5-TAMRA ethylenediamine | 552 | 578 | 90000 | 0.32 | 0.178 |
Citations
View all 3 citations: Citation Explorer
Multicellular Vascularized Engineered Tissues through User-Programmable Biomaterial Photodegradation
Authors: Arakawa, Christopher K and Badeau, Barry A and Zheng, Ying and DeForest, Cole A
Journal: Advanced Materials (2017)
Authors: Arakawa, Christopher K and Badeau, Barry A and Zheng, Ying and DeForest, Cole A
Journal: Advanced Materials (2017)
Green tea inhibited the elimination of nephro-cardiovascular toxins and deteriorated the renal function in rats with renal failure
Authors: Peng, Yu-Hsuan and Sweet, Douglas H and Lin, Shiuan-Pey and Yu, Chung-Ping and Chao, Pei-Dawn Lee and Hou, Yu-Chi
Journal: Scientific reports (2015)
Authors: Peng, Yu-Hsuan and Sweet, Douglas H and Lin, Shiuan-Pey and Yu, Chung-Ping and Chao, Pei-Dawn Lee and Hou, Yu-Chi
Journal: Scientific reports (2015)
Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Preus, Søren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001
Authors: Preus, Søren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001
References
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Low incidence of acute rejection after living-donor liver transplantation: Immunologic analyses by mixed lymphocyte reaction using a carboxyfluorescein diacetate succinimidyl ester labeling technique
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