AAT Bioquest

# 5-FAM ethylenediamine

5-FAM ethylenediamine is an excellent building block for developing fluoresceinated bioconjugates. It has been used for labeling peptides and small biomolecules that contain a carboxy group. It can also be used for labeling carbohydrates or glycoproteins via reductive amination.

## Calculators

### Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of 5-FAM ethylenediamine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

 0.1 mg 0.5 mg 1 mg 5 mg 10 mg 1 mM 187.822 µL 939.108 µL 1.878 mL 9.391 mL 18.782 mL 5 mM 37.564 µL 187.822 µL 375.643 µL 1.878 mL 3.756 mL 10 mM 18.782 µL 93.911 µL 187.822 µL 939.108 µL 1.878 mL

### Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

 Mass (Calculate) Molecular weight Volume (Calculate) Concentration (Calculate) Moles fgpgngµgmgg / g/mol = fLpLnLµLmLL x fMpMnMµMmMM = fmolpmolnmolµmolmmolmol

## Product family

 Name Excitation (nm) Emission (nm) Extinction coefficient (cm -1 M -1) Correction Factor (260 nm) Correction Factor (280 nm) 5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9* 493 517 83000 0.32 0.178 5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] *CAS 92557-80-7* 493 517 83000 0.32 0.178 5-FAM cadaverine 493 517 83000 0.32 0.178 5-FAM Azide 493 517 83000 0.32 0.178 5-FAM Alkyne 493 517 83000 0.32 0.178 5-FAM Cysteamine 493 517 83000 0.32 0.178 5-FITC ethylenediamine [Fluorescein thiocarbamylethylenediamine] *CAS 75453-82-6* 491 516 73000 - 0.35 5-TAMRA ethylenediamine 552 578 90000 0.32 0.178

## Citations

View all 3 citations: Citation Explorer
Multicellular Vascularized Engineered Tissues through User-Programmable Biomaterial Photodegradation
Authors: Arakawa, Christopher K and Badeau, Barry A and Zheng, Ying and DeForest, Cole A
Green tea inhibited the elimination of nephro-cardiovascular toxins and deteriorated the renal function in rats with renal failure
Authors: Peng, Yu-Hsuan and Sweet, Douglas H and Lin, Shiuan-Pey and Yu, Chung-Ping and Chao, Pei-Dawn Lee and Hou, Yu-Chi
Journal: Scientific reports (2015)
Advances in Quantitative FRET-Based Methods for Studying Nucleic Acids
Authors: Preus, S&oslash;ren and Wilhelmsson, L Marcus
Journal: ChemBioChem (2012): 1990--2001

## References

View all 137 references: Citation Explorer
Carboxyfluorescein leakage from poly(ethylene glycol)-grafted liposomes induced by the interaction with serum
Authors: Hashizaki K, Taguchi H, Sakai H, Abe M, Saito Y, Ogawa N.
Journal: Chem Pharm Bull (Tokyo) (2006): 80
Quantifying lymphocyte kinetics in vivo using carboxyfluorescein diacetate succinimidyl ester (cfse)
Authors: Asquith B, Debacq C, Florins A, Gillet N, Sanchez-Alcaraz T, Mosley A, Willems L.
Journal: Proc Biol Sci (2006): 1165
Evidence for dimer formation by an amphiphilic heptapeptide that mediates chloride and carboxyfluorescein release from liposomes
Authors: Pajewski R, Ferdani R, Pajewska J, Djedovic N, Schlesinger PH, Gokel GW.
Journal: Org Biomol Chem (2005): 619
Carboxyfluorescein diacetate succinimidyl ester fluorescent dye for cell labeling
Authors: Wang XQ, Duan XM, Liu LH, Fang YQ, Tan Y.
Journal: Acta Biochim Biophys Sin (Shanghai) (2005): 379
Low incidence of acute rejection after living-donor liver transplantation: Immunologic analyses by mixed lymphocyte reaction using a carboxyfluorescein diacetate succinimidyl ester labeling technique
Authors: Tanaka Y, Ohdan H, Onoe T, Mitsuta H, Tashiro H, Itamoto T, Asahara T.
Journal: Transplantation (2005): 1262
Page updated on September 15, 2024

## Ordering information

 Price Unit size Catalog Number 124 Quantity

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532.42

DMSO

0.32

0.178

83000

493

517

## Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

### Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501