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Fluorescein aldehyde
5-FAM aldehyde
Fluorescein aldehyde is a reactive fluorescent dye that can react with an amine, hydrazine or hydroxylamine. Fluorescein is the most widely used green fluorescent dye. Aldehyde group is reactive toward amines, hydrazide or hydroxylamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldehyde and other groups allows site-specific conjugation and labeling of fluorescein dyes to desired position on targeted molecules. Conjugated fluorescein dye can be easily detected by a common fluorescence instrument under FITC channel.
Chemical structure for Fluorescein aldehyde [5-FAM aldehyde].
Chemical structure for Fluorescein aldehyde [5-FAM aldehyde].
CatalogSize
Price
Quantity
90005 mg
Price
90011 mg
Price
 
Physical properties

Molecular weight550.52
SolventDMSO
Spectral properties

Correction factor (260 nm)0.32
Correction factor (280 nm)0.178
Extinction coefficient (cm -1 M -1)
83000
Excitation (nm)493
Emission (nm)517
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
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Page updated on January 23, 2025