Fluorescein aldehyde [5-FAM aldehyde]

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	550.52
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

550.52

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.178

Extinction coefficient (cm ^-1 M ^-1)

83000

Excitation (nm)

493

Emission (nm)

517

Fluorescein aldehyde is a reactive fluorescent dye that can react with an amine, hydrazine or hydroxylamine. Fluorescein is the most widely used green fluorescent dye. Aldehyde group is reactive toward amines, hydrazide or hydroxylamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldehyde and other groups allows site-specific conjugation and labeling of fluorescein dyes to desired position on targeted molecules. Conjugated fluorescein dye can be easily detected by a common fluorescence instrument under FITC channel.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fluorescein aldehyde [5-FAM aldehyde] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	181.646 µL	908.232 µL	1.816 mL	9.082 mL	18.165 mL
5 mM	36.329 µL	181.646 µL	363.293 µL	1.816 mL	3.633 mL
10 mM	18.165 µL	90.823 µL	181.646 µL	908.232 µL	1.816 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Fluorescein biotin	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein dicaprylate [Fluorescein dioctanoate]	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein DHPE	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Rhodamine aldehyde [5-TAMRA aldehyde]	552	578	90000	-	0.32	0.178
Cy3 aldehyde	555	569	150000¹	0.15¹	0.07	0.073
Cy5 aldehyde	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
XFD488 aldehyde Same Structure to Alexa Fluor™ 488 aldehyde	499	520	73000	0.92¹	-	-
Fluorescein Tyramide	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35

Images

Figure 1. Chemical structure for Fluorescein aldehyde [5-FAM aldehyde].

References

View all 50 references: Citation Explorer

Preparation and optical parameter characterization of two aldehyde derivative thin films for photonic applications by drop casting method.
Authors: Melavanki, Raveendra and Vaijayanthimala, S and Yallur, Basappa Chanabasappa and Shelar, Vikas M and Singh, Diksha and Sadasivuni, Kishor Kumar and Patil, N R
Journal: Luminescence : the journal of biological and chemical luminescence (2020): 903-912

Aldehyde dehydrogenase-2 activation decreases acetaminophen hepatotoxicity by prevention of mitochondrial depolarization.
Authors: Wimborne, Hereward J and Hu, Jiangting and Takemoto, Kenji and Nguyen, Nga T and Jaeschke, Hartmut and Lemasters, John J and Zhong, Zhi
Journal: Toxicology and applied pharmacology (2020): 114982

Thiophene-Based Aldehyde Derivatives for Functionalizable and Adhesive Semiconducting Polymers.
Authors: Istif, Emin and Mantione, Daniele and Vallan, Lorenzo and Hadziioannou, Georges and Brochon, Cyril and Cloutet, Eric and Pavlopoulou, Eleni
Journal: ACS applied materials & interfaces (2020): 8695-8703

Citral Inhibition of Human Salivary Aldehyde Dehydrogenase.
Authors: Laskar, Amaj Ahmed and Khan, Masood Alam and Ahmad, Sumbul and Hashmi, Amiruddin and Younus, Hina
Journal: Cell biochemistry and biophysics (2020): 31-42

Bisretinoid phospholipid and vitamin A aldehyde: Shining a light.
Authors: Kim, Hye Jin and Sparrow, Janet R
Journal: Journal of lipid research (2020)

Didactic approach recounting advances and limitations in novel glutathione and cysteine detection (reduced GSH probe) with mixed coumarin, aldehyde, and phenyl-selenium chemistry.
Authors: Lee, Woohyun and Mulay, Sandip V and Shimodaira, Shingo and Abdillah, Ariq and Palma, Jaymee and Kim, Youngsam and Yudhistira, Tesla and Churchill, David G
Journal: Methods in enzymology (2020): 267-289

Isocyanoacetate-Aldehyde Polymerization: A Facile Tool toward Functional Oxazoline-Containing Polymers.
Authors: Cheng, Tianyu and Chen, Yizhao and Ding, Jie and Qin, Anjun and Tang, Ben Zhong
Journal: Macromolecular rapid communications (2020): e2000179

2-Formyl-dATP as Substrate for Polymerase Synthesis of Reactive DNA Bearing an Aldehyde Group in the Minor Groove.
Authors: Krömer, Matouš and Brunderová, Mária and Ivancová, Ivana and Poštová Slavětínská, Lenka and Hocek, Michal
Journal: ChemPlusChem (2020): 1164-1170

Introduction of an Aldehyde Handle on Nanobodies by Affinity-Guided Labeling.
Authors: Mortensen, Michael R and Skovsgaard, Mikkel B and Märcher, Anders and Andersen, Veronica L and Palmfeldt, Johan and Nielsen, Thorbjørn B and Tørring, Thomas and Laursen, Nick S and Andersen, Kasper R and Kjems, Jørgen and Gothelf, Kurt V
Journal: Bioconjugate chemistry (2020): 1295-1300

Characterization of ascites-derived aldehyde dehydrogenase-positive ovarian cancer stem cells isolated from Leghorn chickens.
Authors: Tiwari, Anupama and Hadley, Jill A and Ramachandran, Ramesh
Journal: Poultry science (2020): 2203-2214