Fluorescein aldehyde [5-FAM aldehyde]

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Physical properties

Molecular weight	550.52
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fluorescein aldehyde [5-FAM aldehyde] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	181.646 µL	908.232 µL	1.816 mL	9.082 mL	18.165 mL
5 mM	36.329 µL	181.646 µL	363.293 µL	1.816 mL	3.633 mL
10 mM	18.165 µL	90.823 µL	181.646 µL	908.232 µL	1.816 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Fluorescein biotin	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein dicaprylate [Fluorescein dioctanoate]	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein DHPE	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Rhodamine aldehyde [5-TAMRA aldehyde]	552	578	90000	-	0.32	0.178
Cy3 aldehyde	555	569	150000¹	0.15¹	0.07	0.073
Cy5 aldehyde	651	670	250000¹	0.27¹, 0.4²	0.02	0.03
XFD488 aldehyde Same Structure to Alexa Fluor™ 488 aldehyde	499	520	71000	0.92¹	0.30	0.11
Fluorescein Tyramide	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35

Images

Figure 1. Chemical structure for Fluorescein aldehyde [5-FAM aldehyde].

References

View all 50 references: Citation Explorer

Preparation and optical parameter characterization of two aldehyde derivative thin films for photonic applications by drop casting method.
Authors: Melavanki, Raveendra and Vaijayanthimala, S and Yallur, Basappa Chanabasappa and Shelar, Vikas M and Singh, Diksha and Sadasivuni, Kishor Kumar and Patil, N R
Journal: Luminescence : the journal of biological and chemical luminescence (2020): 903-912

Aldehyde dehydrogenase-2 activation decreases acetaminophen hepatotoxicity by prevention of mitochondrial depolarization.
Authors: Wimborne, Hereward J and Hu, Jiangting and Takemoto, Kenji and Nguyen, Nga T and Jaeschke, Hartmut and Lemasters, John J and Zhong, Zhi
Journal: Toxicology and applied pharmacology (2020): 114982

Thiophene-Based Aldehyde Derivatives for Functionalizable and Adhesive Semiconducting Polymers.
Authors: Istif, Emin and Mantione, Daniele and Vallan, Lorenzo and Hadziioannou, Georges and Brochon, Cyril and Cloutet, Eric and Pavlopoulou, Eleni
Journal: ACS applied materials & interfaces (2020): 8695-8703

Citral Inhibition of Human Salivary Aldehyde Dehydrogenase.
Authors: Laskar, Amaj Ahmed and Khan, Masood Alam and Ahmad, Sumbul and Hashmi, Amiruddin and Younus, Hina
Journal: Cell biochemistry and biophysics (2020): 31-42

Bisretinoid phospholipid and vitamin A aldehyde: Shining a light.
Authors: Kim, Hye Jin and Sparrow, Janet R
Journal: Journal of lipid research (2020)

Didactic approach recounting advances and limitations in novel glutathione and cysteine detection (reduced GSH probe) with mixed coumarin, aldehyde, and phenyl-selenium chemistry.
Authors: Lee, Woohyun and Mulay, Sandip V and Shimodaira, Shingo and Abdillah, Ariq and Palma, Jaymee and Kim, Youngsam and Yudhistira, Tesla and Churchill, David G
Journal: Methods in enzymology (2020): 267-289

Isocyanoacetate-Aldehyde Polymerization: A Facile Tool toward Functional Oxazoline-Containing Polymers.
Authors: Cheng, Tianyu and Chen, Yizhao and Ding, Jie and Qin, Anjun and Tang, Ben Zhong
Journal: Macromolecular rapid communications (2020): e2000179

2-Formyl-dATP as Substrate for Polymerase Synthesis of Reactive DNA Bearing an Aldehyde Group in the Minor Groove.
Authors: Krömer, Matouš and Brunderová, Mária and Ivancová, Ivana and Poštová Slavětínská, Lenka and Hocek, Michal
Journal: ChemPlusChem (2020): 1164-1170

Introduction of an Aldehyde Handle on Nanobodies by Affinity-Guided Labeling.
Authors: Mortensen, Michael R and Skovsgaard, Mikkel B and Märcher, Anders and Andersen, Veronica L and Palmfeldt, Johan and Nielsen, Thorbjørn B and Tørring, Thomas and Laursen, Nick S and Andersen, Kasper R and Kjems, Jørgen and Gothelf, Kurt V
Journal: Bioconjugate chemistry (2020): 1295-1300

Characterization of ascites-derived aldehyde dehydrogenase-positive ovarian cancer stem cells isolated from Leghorn chickens.
Authors: Tiwari, Anupama and Hadley, Jill A and Ramachandran, Ramesh
Journal: Poultry science (2020): 2203-2214