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5-FAM
aldehyde
Fluorescein aldehyde is a reactive fluorescent dye that can react with an amine, hydrazine or hydroxylamine. Fluorescein is the most widely used green fluorescent dye. Aldehyde group is reactive toward amines, hydrazide or hydroxylamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with N-terminal amine groups at acidic pH, a condition sometimes required for certain bioconjugation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldehyde and other groups allows site-specific conjugation and labeling of fluorescein dyes to desired position on targeted molecules. Conjugated fluorescein dye can be easily detected by a common fluorescence instrument under FITC channel.
Spectrum
Alternative formats
| Name | Form |
| 5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5* | 5-FAM |
| 5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5* | 5-FAM |
| 5-FAM [5-Carboxyfluorescein] *CAS 76823-03-5* | 5-FAM |
| 5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] *CAS 92557-80-7* | 5-FAM succinimidyl ester |
| 5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] *CAS 92557-80-7* | 5-FAM succinimidyl ester |
| 5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] *CAS 92557-80-7* | 5-FAM succinimidyl ester |
| 5-FAM Azide | 5-FAM azide |
| 5-FAM Azide | 5-FAM azide |
| 5-FAM cadaverine | 5-FAM cadaverine |
Show More (6) | |
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Fluorescein biotin | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
| Fluorescein DHPE | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
| Fluorescein dicaprylate [Fluorescein dioctanoate] | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
References
View all 50 references: Citation Explorer
Preparation and optical parameter characterization of two aldehyde derivative thin films for photonic applications by drop casting method.
Authors: Melavanki, Raveendra and Vaijayanthimala, S and Yallur, Basappa Chanabasappa and Shelar, Vikas M and Singh, Diksha and Sadasivuni, Kishor Kumar and Patil, N R
Journal: Luminescence : the journal of biological and chemical luminescence (2020): 903-912
Authors: Melavanki, Raveendra and Vaijayanthimala, S and Yallur, Basappa Chanabasappa and Shelar, Vikas M and Singh, Diksha and Sadasivuni, Kishor Kumar and Patil, N R
Journal: Luminescence : the journal of biological and chemical luminescence (2020): 903-912
Establishing sample-preparation protocols for X-ray phase-contrast CT of rodent spinal cords: Aldehyde fixations and osmium impregnation.
Authors: Barbone, Giacomo E and Bravin, Alberto and Mittone, Alberto and Kraiger, Markus J and Hrabě de Angelis, Martin and Bossi, Mario and Ballarini, Elisa and Rodriguez-Menendez, Virginia and Ceresa, Cecilia and Cavaletti, Guido and Coan, Paola
Journal: Journal of neuroscience methods (2020): 108744
Authors: Barbone, Giacomo E and Bravin, Alberto and Mittone, Alberto and Kraiger, Markus J and Hrabě de Angelis, Martin and Bossi, Mario and Ballarini, Elisa and Rodriguez-Menendez, Virginia and Ceresa, Cecilia and Cavaletti, Guido and Coan, Paola
Journal: Journal of neuroscience methods (2020): 108744
Characterization of ascites-derived aldehyde dehydrogenase-positive ovarian cancer stem cells isolated from Leghorn chickens.
Authors: Tiwari, Anupama and Hadley, Jill A and Ramachandran, Ramesh
Journal: Poultry science (2020): 2203-2214
Authors: Tiwari, Anupama and Hadley, Jill A and Ramachandran, Ramesh
Journal: Poultry science (2020): 2203-2214
Introduction of an Aldehyde Handle on Nanobodies by Affinity-Guided Labeling.
Authors: Mortensen, Michael R and Skovsgaard, Mikkel B and Märcher, Anders and Andersen, Veronica L and Palmfeldt, Johan and Nielsen, Thorbjørn B and Tørring, Thomas and Laursen, Nick S and Andersen, Kasper R and Kjems, Jørgen and Gothelf, Kurt V
Journal: Bioconjugate chemistry (2020): 1295-1300
Authors: Mortensen, Michael R and Skovsgaard, Mikkel B and Märcher, Anders and Andersen, Veronica L and Palmfeldt, Johan and Nielsen, Thorbjørn B and Tørring, Thomas and Laursen, Nick S and Andersen, Kasper R and Kjems, Jørgen and Gothelf, Kurt V
Journal: Bioconjugate chemistry (2020): 1295-1300
2-Formyl-dATP as Substrate for Polymerase Synthesis of Reactive DNA Bearing an Aldehyde Group in the Minor Groove.
Authors: Krömer, Matouš and Brunderová, Mária and Ivancová, Ivana and Poštová Slavětínská, Lenka and Hocek, Michal
Journal: ChemPlusChem (2020): 1164-1170
Authors: Krömer, Matouš and Brunderová, Mária and Ivancová, Ivana and Poštová Slavětínská, Lenka and Hocek, Michal
Journal: ChemPlusChem (2020): 1164-1170
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