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5-FAM Cysteamine

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Physical properties
Molecular weight435.45
SolventDMF
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure

OverviewpdfSDSpdfProtocol


Molecular weight
435.45
5-Carboxyfluorescein (also known as 5-FAM or 5-Fluorescein) is a fluorescent dye commonly used in molecular biology and biochemistry research. It belongs to the family of fluorescein derivatives and is often used as a fluorescent label or tracer in various applications. 5-FAM cysteamine contains a free thiol group that readily reacts with maleimide and other SH-reactive groups such as iodoacetamide and vinyl sulfone etc. It is an excellent building block for developing fluorescent probes with maleimide-modified biomolecules. The resulting probes exhibit strong fluorescence when excited with light in the blue to green spectral range (absorption maximum at approximately 495 nm and emission maximum at approximately 520 nm). Due to its bright fluorescence, stability, and compatibility with various detection systems, 5-FAM probes are widely used as fluorescent labels for biomolecules. Overall, 5-Carboxyfluorescein is a versatile and widely used fluorescent dye in molecular biology research, offering excellent brightness and compatibility with various labeling and detection methods. Its fluorescent properties make it a valuable tool for visualizing and studying biological molecules and processes. It can be employed to label nucleotides, oligonucleotides, peptides, proteins, antibodies, or other molecules of interest. The labeled molecules can be visualized and detected using fluorescence microscopy, flow cytometry, or other fluorescence-based techniques.

Calculators


Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of 5-FAM Cysteamine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM229.647 µL1.148 mL2.296 mL11.482 mL22.965 mL
5 mM45.929 µL229.647 µL459.295 µL2.296 mL4.593 mL
10 mM22.965 µL114.824 µL229.647 µL1.148 mL2.296 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9*493517830000.320.178
5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] *CAS 92557-80-7*493517830000.320.178
5-FAM ethylenediamine493517830000.320.178
5-FAM cadaverine493517830000.320.178
5-FAM Azide493517830000.320.178
5-FAM Alkyne493517830000.320.178

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References


View all 50 references: Citation Explorer
Development of Thiol-Maleimide hydrogels incorporating graphene-based nanomaterials for cancer chemo-photothermal therapy.
Authors: Costa, Francisco J P and Nave, Micaela and Lima-Sousa, Rita and Alves, Cátia G and Melo, Bruna L and Correia, Ilídio J and de Melo-Diogo, Duarte
Journal: International journal of pharmaceutics (2023): 122713
Nanoscale Control of DNA-Linked MoS2-Quantum Dot Heterostructures.
Authors: Talha-Dean, Teymour and Chen, Kai and Mastroianni, Giulia and Gesuele, Felice and Mol, Jan and Palma, Matteo
Journal: Bioconjugate chemistry (2023): 78-84
Design and Development of Microformulations for Rapid Release of Small Molecules and Oligonucleotides.
Authors: Brown, T and Stanton, M and Cros, F and Cho, S and Kiselyov, A
Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (2023): 106472
A unique reaction of diphenylcyclopropenone and 1,2-aminothiol with the release of thiol for multiple bioconjugation.
Authors: Liu, Shanshan and Ye, Haishun and Yi, Long and Xi, Zhen
Journal: Chemical communications (Cambridge, England) (2023): 1497-1500
Bioengineering of air-filled protein nanoparticles by genetic and chemical functionalization.
Authors: Karan, Ram and Renn, Dominik and Nozue, Shuho and Zhao, Lingyun and Habuchi, Satoshi and Allers, Thorsten and Rueping, Magnus
Journal: Journal of nanobiotechnology (2023): 108
New method for peptide purification based on selective removal of truncation peptide impurities after SPPS with orthogonal capping.
Authors: Wunderlich, Hendrik and Alvaro, Roque Augusto Castro and Wenschuh, Holger and Schnatbaum, Karsten
Journal: Journal of peptide science : an official publication of the European Peptide Society (2023): e3496
Multiresponsive Keratin-Polysulfobetaine Conjugate-Based Micelles as Drug Carriers with a Prolonged Circulation Time.
Authors: Sun, Yu and Liu, Pengcheng and Zhang, Jie and Wang, Lijuan and Shang, Yushuang and Shen, Jian and Yuan, Jiang
Journal: Langmuir : the ACS journal of surfaces and colloids (2023): 5418-5425
Antimicrobial peptide-grafted PLGA-PEG nanoparticles to fight bacterial wound infections.
Authors: Ramôa, António Miguel and Campos, Filipa and Moreira, Luís and Teixeira, Cátia and Leiro, Victoria and Gomes, Paula and das Neves, José and Martins, M Cristina L and Monteiro, Cláudia
Journal: Biomaterials science (2023): 499-508
A Chemical Strategy for the Preparation of Multimodified Peptide Imaging Probes.
Authors: De Rosa, Lucia and Hawala, Ivan and Di Stasi, Rossella and Stefania, Rachele and Capozza, Martina and Nava, Donatella and D'Andrea, Luca Domenico
Journal: The Journal of organic chemistry (2023): 4546-4553
Improved Metal-Free Approach for the Synthesis of Protected Thiol Containing Thymidine Nucleoside Phosphoramidite and Its Application for the Synthesis of Ligatable Oligonucleotide Conjugates.
Authors: Kupihár, Zoltán and Ferenc, Györgyi and Petrovicz, Vencel L and Fáy, Viktória R and Kovács, Lajos and Martinek, Tamás A and Hegedüs, Zsófia
Journal: Pharmaceutics (2023)