5-FAM Cysteamine
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 435.45 |
Solvent | DMF |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Related products
Overview | ![]() ![]() |
Molecular weight 435.45 |
5-Carboxyfluorescein (also known as 5-FAM or 5-Fluorescein) is a fluorescent dye commonly used in molecular biology and biochemistry research. It belongs to the family of fluorescein derivatives and is often used as a fluorescent label or tracer in various applications. 5-FAM cysteamine contains a free thiol group that readily reacts with maleimide and other SH-reactive groups such as iodoacetamide and vinyl sulfone etc. It is an excellent building block for developing fluorescent probes with maleimide-modified biomolecules. The resulting probes exhibit strong fluorescence when excited with light in the blue to green spectral range (absorption maximum at approximately 495 nm and emission maximum at approximately 520 nm). Due to its bright fluorescence, stability, and compatibility with various detection systems, 5-FAM probes are widely used as fluorescent labels for biomolecules. Overall, 5-Carboxyfluorescein is a versatile and widely used fluorescent dye in molecular biology research, offering excellent brightness and compatibility with various labeling and detection methods. Its fluorescent properties make it a valuable tool for visualizing and studying biological molecules and processes. It can be employed to label nucleotides, oligonucleotides, peptides, proteins, antibodies, or other molecules of interest. The labeled molecules can be visualized and detected using fluorescence microscopy, flow cytometry, or other fluorescence-based techniques.
Calculators
Common stock solution preparation
Table 1. Volume of DMF needed to reconstitute specific mass of 5-FAM Cysteamine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 229.647 µL | 1.148 mL | 2.296 mL | 11.482 mL | 22.965 mL |
5 mM | 45.929 µL | 229.647 µL | 459.295 µL | 2.296 mL | 4.593 mL |
10 mM | 22.965 µL | 114.824 µL | 229.647 µL | 1.148 mL | 2.296 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (260 nm) | Correction Factor (280 nm) |
5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9* | 493 | 517 | 83000 | 0.32 | 0.178 |
5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] *CAS 92557-80-7* | 493 | 517 | 83000 | 0.32 | 0.178 |
5-FAM ethylenediamine | 493 | 517 | 83000 | 0.32 | 0.178 |
5-FAM cadaverine | 493 | 517 | 83000 | 0.32 | 0.178 |
5-FAM Azide | 493 | 517 | 83000 | 0.32 | 0.178 |
5-FAM Alkyne | 493 | 517 | 83000 | 0.32 | 0.178 |
Images
References
View all 50 references: Citation Explorer
Development of Thiol-Maleimide hydrogels incorporating graphene-based nanomaterials for cancer chemo-photothermal therapy.
Authors: Costa, Francisco J P and Nave, Micaela and Lima-Sousa, Rita and Alves, Cátia G and Melo, Bruna L and Correia, Ilídio J and de Melo-Diogo, Duarte
Journal: International journal of pharmaceutics (2023): 122713
Authors: Costa, Francisco J P and Nave, Micaela and Lima-Sousa, Rita and Alves, Cátia G and Melo, Bruna L and Correia, Ilídio J and de Melo-Diogo, Duarte
Journal: International journal of pharmaceutics (2023): 122713
In situ albumin-binding and esterase-specifically cleaved BRD4-degrading PROTAC for targeted cancer therapy.
Authors: Cho, Hanhee and Jeon, Seong Ik and Shim, Man Kyu and Ahn, Cheol-Hee and Kim, Kwangmeyung
Journal: Biomaterials (2023): 122038
Authors: Cho, Hanhee and Jeon, Seong Ik and Shim, Man Kyu and Ahn, Cheol-Hee and Kim, Kwangmeyung
Journal: Biomaterials (2023): 122038
Functionalisation of extracellular vesicles with cyclic-RGDyC potentially for glioblastoma targeted intracellular drug delivery.
Authors: Geng, Tianjiao and Leung, Euphemia and Chamley, Lawrence W and Wu, Zimei
Journal: Biomaterials advances (2023): 213388
Authors: Geng, Tianjiao and Leung, Euphemia and Chamley, Lawrence W and Wu, Zimei
Journal: Biomaterials advances (2023): 213388
Enhancing the Activity of Surface Immobilized Antimicrobial Peptides Using Thiol-Mediated Tethering to Poly(ethylene glycol).
Authors: Boden, Andrew and Dart, Alexander and Liao, Tzu-Ying and Zhu, De Ming and Bhave, Mrinal and Cipolla, Laura and Kingshott, Peter
Journal: Macromolecular bioscience (2023): e2200411
Authors: Boden, Andrew and Dart, Alexander and Liao, Tzu-Ying and Zhu, De Ming and Bhave, Mrinal and Cipolla, Laura and Kingshott, Peter
Journal: Macromolecular bioscience (2023): e2200411
Comparison of methods generating antibody-epitope conjugates for targeting cancer with virus-specific T cells.
Authors: van der Wulp, Willemijn and Gram, Anna M and Bleijlevens, Boris and Hagedoorn, Renate S and Araman, Can and Kim, Robbert Q and Drijfhout, Jan Wouter and Parren, Paul W H I and Hibbert, Richard G and Hoeben, Rob C and van Kasteren, Sander I and Schuurman, Janine and Ressing, Maaike E and Heemskerk, Mirjam H M
Journal: Frontiers in immunology (2023): 1183914
Authors: van der Wulp, Willemijn and Gram, Anna M and Bleijlevens, Boris and Hagedoorn, Renate S and Araman, Can and Kim, Robbert Q and Drijfhout, Jan Wouter and Parren, Paul W H I and Hibbert, Richard G and Hoeben, Rob C and van Kasteren, Sander I and Schuurman, Janine and Ressing, Maaike E and Heemskerk, Mirjam H M
Journal: Frontiers in immunology (2023): 1183914
Structure-Activity Relationships of RGD-Containing Peptides in Integrin αvβ5-Mediated Cell Adhesion.
Authors: Yamada, Yuji and Onda, Toru and Wada, Yuri and Hamada, Keisuke and Kikkawa, Yamato and Nomizu, Motoyoshi
Journal: ACS omega (2023): 4687-4693
Authors: Yamada, Yuji and Onda, Toru and Wada, Yuri and Hamada, Keisuke and Kikkawa, Yamato and Nomizu, Motoyoshi
Journal: ACS omega (2023): 4687-4693
Total Chemical Synthesis of Palmitoyl-Conjugated Insulin.
Authors: Liu, Mengjie and Li, Qingyang and Delaine, Carlie and Wu, Hongkang and Arsenakis, Yanni and White, Barbara F and Forbes, Briony E and Chandrashekar, Chaitra and Hossain, Mohammed Akhter
Journal: ACS omega (2023): 13715-13720
Authors: Liu, Mengjie and Li, Qingyang and Delaine, Carlie and Wu, Hongkang and Arsenakis, Yanni and White, Barbara F and Forbes, Briony E and Chandrashekar, Chaitra and Hossain, Mohammed Akhter
Journal: ACS omega (2023): 13715-13720
Generation and structure-activity relationships of novel imidazo-thienopyridine based TLR7 agonists: application as payloads for immunostimulatory antibody drug-conjugates.
Authors: Brant, Michael G and Garnett, Graham A E and Guedia, Joy and Lasalle, Manuel and Lawn, Samuel and Petersen, Mark E and Duan, Renee and Mendez-Campos, José and Hirkala-Schaefer, Truman and Winters, Geoffrey C and Barnscher, Stuart D
Journal: Bioorganic & medicinal chemistry letters (2023): 129348
Authors: Brant, Michael G and Garnett, Graham A E and Guedia, Joy and Lasalle, Manuel and Lawn, Samuel and Petersen, Mark E and Duan, Renee and Mendez-Campos, José and Hirkala-Schaefer, Truman and Winters, Geoffrey C and Barnscher, Stuart D
Journal: Bioorganic & medicinal chemistry letters (2023): 129348
Improving the stability of thiol-maleimide bioconjugates via the formation of a thiazine structure.
Authors: Gober, Isaiah N and Sharan, Rahul and Villain, Matteo
Journal: Journal of peptide science : an official publication of the European Peptide Society (2023): e3495
Authors: Gober, Isaiah N and Sharan, Rahul and Villain, Matteo
Journal: Journal of peptide science : an official publication of the European Peptide Society (2023): e3495
Minibody-Based and scFv-Based Antibody Fragment-Drug Conjugates Selectively Eliminate GD2-Positive Tumor Cells.
Authors: Kalinovsky, Daniel V and Kholodenko, Irina V and Kibardin, Alexey V and Doronin, Igor I and Svirshchevskaya, Elena V and Ryazantsev, Dmitriy Y and Konovalova, Maria V and Rozov, Fedor N and Larin, Sergey S and Deyev, Sergey M and Kholodenko, Roman V
Journal: International journal of molecular sciences (2023)
Authors: Kalinovsky, Daniel V and Kholodenko, Irina V and Kibardin, Alexey V and Doronin, Igor I and Svirshchevskaya, Elena V and Ryazantsev, Dmitriy Y and Konovalova, Maria V and Rozov, Fedor N and Larin, Sergey S and Deyev, Sergey M and Kholodenko, Roman V
Journal: International journal of molecular sciences (2023)