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5-FAM Cysteamine

5-Carboxyfluorescein (also known as 5-FAM or 5-Fluorescein) is a fluorescent dye commonly used in molecular biology and biochemistry research. It belongs to the family of fluorescein derivatives and is often used as a fluorescent label or tracer in various applications. 5-FAM cysteamine contains a free thiol group that readily reacts with maleimide and other SH-reactive groups such as iodoacetamide and vinyl sulfone etc. It is an excellent building block for developing fluorescent probes with maleimide-modified biomolecules. The resulting probes exhibit strong fluorescence when excited with light in the blue to green spectral range (absorption maximum at approximately 495 nm and emission maximum at approximately 520 nm). Due to its bright fluorescence, stability, and compatibility with various detection systems, 5-FAM probes are widely used as fluorescent labels for biomolecules. Overall, 5-Carboxyfluorescein is a versatile and widely used fluorescent dye in molecular biology research, offering excellent brightness and compatibility with various labeling and detection methods. Its fluorescent properties make it a valuable tool for visualizing and studying biological molecules and processes. It can be employed to label nucleotides, oligonucleotides, peptides, proteins, antibodies, or other molecules of interest. The labeled molecules can be visualized and detected using fluorescence microscopy, flow cytometry, or other fluorescence-based techniques.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (260 nm)Correction Factor (280 nm)
5(6)-FAM [5-(and-6)-Carboxyfluorescein] *CAS 72088-94-9*493517830000.320.178
5-FAM, SE [5-Carboxyfluorescein, succinimidyl ester] *CAS 92557-80-7*493517830000.320.178
5-FAM ethylenediamine493517830000.320.178
5-FAM cadaverine493517830000.320.178
5-FAM Azide493517830000.320.178
5-FAM Alkyne493517830000.320.178

References

View all 50 references: Citation Explorer
Development of Thiol-Maleimide hydrogels incorporating graphene-based nanomaterials for cancer chemo-photothermal therapy.
Authors: Costa, Francisco J P and Nave, Micaela and Lima-Sousa, Rita and Alves, Cátia G and Melo, Bruna L and Correia, Ilídio J and de Melo-Diogo, Duarte
Journal: International journal of pharmaceutics (2023): 122713
Nanoscale Control of DNA-Linked MoS2-Quantum Dot Heterostructures.
Authors: Talha-Dean, Teymour and Chen, Kai and Mastroianni, Giulia and Gesuele, Felice and Mol, Jan and Palma, Matteo
Journal: Bioconjugate chemistry (2023): 78-84
Design and Development of Microformulations for Rapid Release of Small Molecules and Oligonucleotides.
Authors: Brown, T and Stanton, M and Cros, F and Cho, S and Kiselyov, A
Journal: European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences (2023): 106472
A unique reaction of diphenylcyclopropenone and 1,2-aminothiol with the release of thiol for multiple bioconjugation.
Authors: Liu, Shanshan and Ye, Haishun and Yi, Long and Xi, Zhen
Journal: Chemical communications (Cambridge, England) (2023): 1497-1500
Bioengineering of air-filled protein nanoparticles by genetic and chemical functionalization.
Authors: Karan, Ram and Renn, Dominik and Nozue, Shuho and Zhao, Lingyun and Habuchi, Satoshi and Allers, Thorsten and Rueping, Magnus
Journal: Journal of nanobiotechnology (2023): 108
Page updated on October 8, 2024

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Catalog Number5210
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Physical properties

Molecular weight

435.45

Solvent

DMF

Spectral properties

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.178

Extinction coefficient (cm -1 M -1)

83000

Excitation (nm)

493

Emission (nm)

517

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
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