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6-NED alkyne

5'-Labeled primer pairs include a fluorescently labeled oligo with a choice of dye on the 5' end, along with an unlabeled oligo, in each pair. These products can be used in a variety of molecular biological applications. 6-NED is one of the colors used for making the well-known multicolor fluorescent oligo set of 6-FAM, 6-TET, 6-VIC, 6-HEX, 6-NED and 6-PET for use in fragment analysis, gene expression studies, genotyping, and PCR applications. 6 -NED alkyne is quite stable fluorescent probe that can be readily used for labeling azido-modified oligos.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Correction Factor (280 nm)
6-FAM Alkyne493517830000.178
6-HEX alkyne533559730000.15
6-TET alkyne521542730000.13
6-JOE alkyne520545750001-
6-TAMRA alkyne552578900000.178
6-ROX alkyne578604820000.168
6-NED, SE [6-NED NHS ester]545567740001-
6-VIC Alkyne526543--

References

View all 22 references: Citation Explorer
Assessment of genetic diversity and bottleneck in Purnathadi buffaloes using short tandem repeat markers.
Authors: Ali, S Sajid and Kuralkar, S V and Das, Ramendra and Raina, Varinder and Kataria, R S and Vohra, Vikas
Journal: Animal biotechnology (2020): 1-12
Simultaneous detection and quantification of 19 diarrhea-related pathogens with a quantitative real-time PCR panel assay.
Authors: Wongboot, Warawan and Okada, Kazuhisa and Chantaroj, Siriporn and Kamjumphol, Watcharaporn and Hamada, Shigeyuki
Journal: Journal of microbiological methods (2018): 76-82
HLA Class III: A susceptibility region to systemic lupus erythematosus in Tunisian population.
Authors: Hachicha, Hend and Mahfoudh, Nadia and Fourati, Hajer and Elloumi, Nesrine and Marzouk, Sameh and Feki, Sawsan and Fakhfakh, Raouia and Frikha, Faten and Ayadi, Abir and Maatoug, Amira and Gaddour, Lilia and Hakim, Feiza and Bahloul, Zouheir and Makni, Hafedh and Masmoudi, Hatem and Kammoun, Arwa
Journal: PloS one (2018): e0198549
Multicolor-based discrimination of 21 short tandem repeats and amelogenin using four fluorescent universal primers.
Authors: Asari, Masaru and Okuda, Katsuhiro and Hoshina, Chisato and Omura, Tomohiro and Tasaki, Yoshikazu and Shiono, Hiroshi and Matsubara, Kazuo and Shimizu, Keiko
Journal: Analytical biochemistry (2016): 16-22
Multiplex-Ready Technology for mid-throughput genotyping of molecular markers.
Authors: Bonneau, Julien and Hayden, Matthew
Journal: Methods in molecular biology (Clifton, N.J.) (2014): 47-57
Page updated on October 11, 2024

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Physical properties

Molecular weight

584.76

Solvent

DMSO

Spectral properties

Absorbance (nm)

544

Extinction coefficient (cm -1 M -1)

740001

Excitation (nm)

545

Emission (nm)

567

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501